in vitro model for the growth and analysis of chronic wound MRSA biofilms
Aims: To develop an in vitro model (Colony/drip‐flow reactor – C/DFR) for the growth and analysis of methicillin‐resistant Staphylococcus aureus (MRSA) biofilms. Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical is...
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| Veröffentlicht in: | Journal of applied microbiology 2011-11, Vol.111 (5), p.1275-1282 |
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| container_title | Journal of applied microbiology |
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| creator | Agostinho, A.M Hartman, A Lipp, C Parker, A.E Stewart, P.S James, G.A |
| description | Aims: To develop an in vitro model (Colony/drip‐flow reactor – C/DFR) for the growth and analysis of methicillin‐resistant Staphylococcus aureus (MRSA) biofilms. Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 μm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin’s solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment. Conclusions: The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms. Significance and Impact of the Study: The C/DFR can be used as a tool for examining complex biofilm physiology as well as for performing comparative experiments that test wound care products and novel antimicrobials. |
| doi_str_mv | 10.1111/j.1365-2672.2011.05138.x |
| format | Article |
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Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 μm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin’s solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment. Conclusions: The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms. Significance and Impact of the Study: The C/DFR can be used as a tool for examining complex biofilm physiology as well as for performing comparative experiments that test wound care products and novel antimicrobials.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2011.05138.x</identifier><identifier>PMID: 21883733</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; antimicrobial agents ; biofilm ; biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; chronic wounds ; Drug Combinations ; Fundamental and applied biological sciences. Psychology ; growth models ; in vitro model ; methicillin ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - growth & development ; Microbiology ; Models, Biological ; MRSA ; physiology ; silver ; Silver Sulfadiazine - pharmacology ; Sodium Bicarbonate - pharmacology ; sodium chloride ; Sodium Hypochlorite - pharmacology ; Staphylococcus aureus ; sulfadiazine</subject><ispartof>Journal of applied microbiology, 2011-11, Vol.111 (5), p.1275-1282</ispartof><rights>2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology</rights><rights>2015 INIST-CNRS</rights><rights>2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4238-7f1fd10116f06ffc36b2449e3d425ab07540b4ad1b10cbfd18071d70b2f170c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2011.05138.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2011.05138.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24618632$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21883733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agostinho, A.M</creatorcontrib><creatorcontrib>Hartman, A</creatorcontrib><creatorcontrib>Lipp, C</creatorcontrib><creatorcontrib>Parker, A.E</creatorcontrib><creatorcontrib>Stewart, P.S</creatorcontrib><creatorcontrib>James, G.A</creatorcontrib><title>in vitro model for the growth and analysis of chronic wound MRSA biofilms</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: To develop an in vitro model (Colony/drip‐flow reactor – C/DFR) for the growth and analysis of methicillin‐resistant Staphylococcus aureus (MRSA) biofilms. Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 μm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin’s solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment. Conclusions: The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms. Significance and Impact of the Study: The C/DFR can be used as a tool for examining complex biofilm physiology as well as for performing comparative experiments that test wound care products and novel antimicrobials.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>antimicrobial agents</subject><subject>biofilm</subject><subject>biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>chronic wounds</subject><subject>Drug Combinations</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>growth models</subject><subject>in vitro model</subject><subject>methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - growth & development</subject><subject>Microbiology</subject><subject>Models, Biological</subject><subject>MRSA</subject><subject>physiology</subject><subject>silver</subject><subject>Silver Sulfadiazine - pharmacology</subject><subject>Sodium Bicarbonate - pharmacology</subject><subject>sodium chloride</subject><subject>Sodium Hypochlorite - pharmacology</subject><subject>Staphylococcus aureus</subject><subject>sulfadiazine</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkcFuGyEQhlHVqHHSvkLLpeppNwywCzn0YFlpkspRpMQ9I5aFGGt3ScGO47cPG7su0oiR_m8GZn6EMJAS8rlYlcDqqqC1oCUlACWpgMny9QOaHIWP7zkvKiLoKTpLaUUIMFLVn9ApBSmZYGyCbv2AX_w6BtyH1nbYhYjXS4ufYtiul1gPbQ7d7ZJPODhsljEM3uBt2GTl7uFxihsfnO_69BmdON0l--Vwn6PFr6vF7KaY31_fzqbzwnDKZCEcuBbyn2tHaucMqxvK-aVlLaeVboioOGm4bqEBYpqMSiKgFaShDgQx7Bz92Ld9juHvxqa16n0ytuv0YMMmKXkpJSdAq0x-PZCbpreteo6-13Gn_g2fge8HQCejOxf1YHz6z_EaZM1o5n7uua3v7O6oA1GjGWqlxp2rcedqNEO9m6Fe1e_p3Zjl-m_7eqeD0k8xv_HnMZNVNoQLLih7AxRzhEE</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Agostinho, A.M</creator><creator>Hartman, A</creator><creator>Lipp, C</creator><creator>Parker, A.E</creator><creator>Stewart, P.S</creator><creator>James, G.A</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>in vitro model for the growth and analysis of chronic wound MRSA biofilms</title><author>Agostinho, A.M ; Hartman, A ; Lipp, C ; Parker, A.E ; Stewart, P.S ; James, G.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4238-7f1fd10116f06ffc36b2449e3d425ab07540b4ad1b10cbfd18071d70b2f170c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>antimicrobial agents</topic><topic>biofilm</topic><topic>biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>chronic wounds</topic><topic>Drug Combinations</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>growth models</topic><topic>in vitro model</topic><topic>methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - growth & development</topic><topic>Microbiology</topic><topic>Models, Biological</topic><topic>MRSA</topic><topic>physiology</topic><topic>silver</topic><topic>Silver Sulfadiazine - pharmacology</topic><topic>Sodium Bicarbonate - pharmacology</topic><topic>sodium chloride</topic><topic>Sodium Hypochlorite - pharmacology</topic><topic>Staphylococcus aureus</topic><topic>sulfadiazine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Agostinho, A.M</creatorcontrib><creatorcontrib>Hartman, A</creatorcontrib><creatorcontrib>Lipp, C</creatorcontrib><creatorcontrib>Parker, A.E</creatorcontrib><creatorcontrib>Stewart, P.S</creatorcontrib><creatorcontrib>James, G.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agostinho, A.M</au><au>Hartman, A</au><au>Lipp, C</au><au>Parker, A.E</au><au>Stewart, P.S</au><au>James, G.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>in vitro model for the growth and analysis of chronic wound MRSA biofilms</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2011-11</date><risdate>2011</risdate><volume>111</volume><issue>5</issue><spage>1275</spage><epage>1282</epage><pages>1275-1282</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims: To develop an in vitro model (Colony/drip‐flow reactor – C/DFR) for the growth and analysis of methicillin‐resistant Staphylococcus aureus (MRSA) biofilms. Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 μm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin’s solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment. Conclusions: The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Anti-Bacterial Agents - pharmacology antibiotic resistance antimicrobial agents biofilm biofilms Biofilms - drug effects Biofilms - growth & development Biological and medical sciences chronic wounds Drug Combinations Fundamental and applied biological sciences. Psychology growth models in vitro model methicillin Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - growth & development Microbiology Models, Biological MRSA physiology silver Silver Sulfadiazine - pharmacology Sodium Bicarbonate - pharmacology sodium chloride Sodium Hypochlorite - pharmacology Staphylococcus aureus sulfadiazine |
| title | in vitro model for the growth and analysis of chronic wound MRSA biofilms |
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