XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1
In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits...
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Veröffentlicht in: | Journal of Bacteriology 2011-11, Vol.193 (21), p.6032-6038 |
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description | In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes. |
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In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/JB.05367-11</identifier><identifier>PMID: 21873495</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Alcohol Oxidoreductases - biosynthesis ; Alcohol Oxidoreductases - genetics ; Aldehydes ; bacteria ; Bacteriology ; Biological and medical sciences ; Culture Media - chemistry ; formaldehyde ; Formaldehyde - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Regulation, Bacterial ; genes ; Gram-negative bacteria ; Methanol ; Methanol - metabolism ; Methylobacterium extorquens ; Methylobacterium extorquens - enzymology ; Methylobacterium extorquens - genetics ; Methylobacterium extorquens - growth & development ; Methylobacterium extorquens - metabolism ; Microbiology ; Miscellaneous ; Oxidation ; Physiology and Metabolism ; promoter regions ; Proteins ; Sequence Homology, Amino Acid</subject><ispartof>Journal of Bacteriology, 2011-11, Vol.193 (21), p.6032-6038</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Nov 2011</rights><rights>Copyright © 2011, American Society for Microbiology. All Rights Reserved. 2011 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-691fa369f769c61d26f277d6c6c6e4a283ee664487e1c3083506fe557ecc1823</citedby><cites>FETCH-LOGICAL-c554t-691fa369f769c61d26f277d6c6c6e4a283ee664487e1c3083506fe557ecc1823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194914/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3194914/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24637640$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21873495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Skovran, Elizabeth</creatorcontrib><creatorcontrib>Palmer, Alexander D</creatorcontrib><creatorcontrib>Rountree, Austin M</creatorcontrib><creatorcontrib>Good, Nathan M</creatorcontrib><creatorcontrib>Lidstrom, Mary E</creatorcontrib><title>XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.</description><subject>Alcohol Oxidoreductases - biosynthesis</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Aldehydes</subject><subject>bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Culture Media - chemistry</subject><subject>formaldehyde</subject><subject>Formaldehyde - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>genes</subject><subject>Gram-negative bacteria</subject><subject>Methanol</subject><subject>Methanol - metabolism</subject><subject>Methylobacterium extorquens</subject><subject>Methylobacterium extorquens - enzymology</subject><subject>Methylobacterium extorquens - genetics</subject><subject>Methylobacterium extorquens - growth & development</subject><subject>Methylobacterium extorquens - metabolism</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Oxidation</subject><subject>Physiology and Metabolism</subject><subject>promoter regions</subject><subject>Proteins</subject><subject>Sequence Homology, Amino Acid</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks9rFDEYhgdR7Fo9edcgiAeZmt8zuQhtbbWlRdAKHoSQzXzZzTIz2SYz7e5_b7a7tiqBhPA9vDzhTVG8JPiAEFp_OD86wILJqiTkUTEhWNWlEAw_LiYYU1Iqothe8SylBcaEc0GfFnuU1BXjSkyKXz_D6hSdJfQNrkcfoUEuRHSyWkZIyYceBYcuYZibPrToE8zXTQwz6E0C5Pu7yboNU2MHiH7sEKyGEK9H6BM6vCTPiyfOtAle7M794ur05Or4S3nx9fPZ8eFFaYXgQykVcYZJ5SqprCQNlY5WVSNtXsANrRmAlJzXFRDLcM0Elg6EqMBaUlO2X3zcxi7HaQeNhX6IptXL6DsT1zoYr_-d9H6uZ-FGM6K4IjwHvNsFxJDl06A7nyy0rekhjEnXqhY4b1Um3_xHLsIY-_w4rTCWElOx8Xm_hWwMKUVw9yoE601l-vxI31WWb5l-9bf9Pfunowy83QEmWdO6aHrr0wPHJaskxw9ucz-b3-YutUmdXkx1_gA5TUvMNm6vt5AzQZtZzEE_vlNMxOZzKKUY-w0H77JX</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Skovran, Elizabeth</creator><creator>Palmer, Alexander D</creator><creator>Rountree, Austin M</creator><creator>Good, Nathan M</creator><creator>Lidstrom, Mary E</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111101</creationdate><title>XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1</title><author>Skovran, Elizabeth ; Palmer, Alexander D ; Rountree, Austin M ; Good, Nathan M ; Lidstrom, Mary E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-691fa369f769c61d26f277d6c6c6e4a283ee664487e1c3083506fe557ecc1823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alcohol Oxidoreductases - biosynthesis</topic><topic>Alcohol Oxidoreductases - genetics</topic><topic>Aldehydes</topic><topic>bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Culture Media - chemistry</topic><topic>formaldehyde</topic><topic>Formaldehyde - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>genes</topic><topic>Gram-negative bacteria</topic><topic>Methanol</topic><topic>Methanol - metabolism</topic><topic>Methylobacterium extorquens</topic><topic>Methylobacterium extorquens - enzymology</topic><topic>Methylobacterium extorquens - genetics</topic><topic>Methylobacterium extorquens - growth & development</topic><topic>Methylobacterium extorquens - metabolism</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Oxidation</topic><topic>Physiology and Metabolism</topic><topic>promoter regions</topic><topic>Proteins</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skovran, Elizabeth</creatorcontrib><creatorcontrib>Palmer, Alexander D</creatorcontrib><creatorcontrib>Rountree, Austin M</creatorcontrib><creatorcontrib>Good, Nathan M</creatorcontrib><creatorcontrib>Lidstrom, Mary E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skovran, Elizabeth</au><au>Palmer, Alexander D</au><au>Rountree, Austin M</au><au>Good, Nathan M</au><au>Lidstrom, Mary E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>193</volume><issue>21</issue><spage>6032</spage><epage>6038</epage><pages>6032-6038</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>21873495</pmid><doi>10.1128/JB.05367-11</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Alcohol Oxidoreductases - biosynthesis Alcohol Oxidoreductases - genetics Aldehydes bacteria Bacteriology Biological and medical sciences Culture Media - chemistry formaldehyde Formaldehyde - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Bacterial genes Gram-negative bacteria Methanol Methanol - metabolism Methylobacterium extorquens Methylobacterium extorquens - enzymology Methylobacterium extorquens - genetics Methylobacterium extorquens - growth & development Methylobacterium extorquens - metabolism Microbiology Miscellaneous Oxidation Physiology and Metabolism promoter regions Proteins Sequence Homology, Amino Acid |
title | XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1 |
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