XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1

In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits...

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Veröffentlicht in:Journal of Bacteriology 2011-11, Vol.193 (21), p.6032-6038
Hauptverfasser: Skovran, Elizabeth, Palmer, Alexander D, Rountree, Austin M, Good, Nathan M, Lidstrom, Mary E
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container_issue 21
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container_title Journal of Bacteriology
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creator Skovran, Elizabeth
Palmer, Alexander D
Rountree, Austin M
Good, Nathan M
Lidstrom, Mary E
description In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.
doi_str_mv 10.1128/JB.05367-11
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In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ∼50% identical to MxaF and ∼90% identical to each other. It was previously reported that xoxF is not required for methanol growth in M. extorquens AM1, but here we show that when both xoxF homologs are absent, strains are unable to grow in methanol medium and lack methanol dehydrogenase activity. We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/JB.05367-11</identifier><identifier>PMID: 21873495</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Alcohol Oxidoreductases - biosynthesis ; Alcohol Oxidoreductases - genetics ; Aldehydes ; bacteria ; Bacteriology ; Biological and medical sciences ; Culture Media - chemistry ; formaldehyde ; Formaldehyde - metabolism ; Fundamental and applied biological sciences. 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Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>genes</topic><topic>Gram-negative bacteria</topic><topic>Methanol</topic><topic>Methanol - metabolism</topic><topic>Methylobacterium extorquens</topic><topic>Methylobacterium extorquens - enzymology</topic><topic>Methylobacterium extorquens - genetics</topic><topic>Methylobacterium extorquens - growth &amp; development</topic><topic>Methylobacterium extorquens - metabolism</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Oxidation</topic><topic>Physiology and Metabolism</topic><topic>promoter regions</topic><topic>Proteins</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skovran, Elizabeth</creatorcontrib><creatorcontrib>Palmer, Alexander D</creatorcontrib><creatorcontrib>Rountree, Austin M</creatorcontrib><creatorcontrib>Good, Nathan M</creatorcontrib><creatorcontrib>Lidstrom, Mary E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skovran, Elizabeth</au><au>Palmer, Alexander D</au><au>Rountree, Austin M</au><au>Good, Nathan M</au><au>Lidstrom, Mary E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>193</volume><issue>21</issue><spage>6032</spage><epage>6038</epage><pages>6032-6038</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. 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We demonstrate that these defects result from the loss of gene expression from the mxa promoter and suggest that XoxF is part of a complex regulatory cascade involving the 2-component systems MxcQE and MxbDM, which are required for the expression of the methanol dehydrogenase genes.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>21873495</pmid><doi>10.1128/JB.05367-11</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Alcohol Oxidoreductases - biosynthesis
Alcohol Oxidoreductases - genetics
Aldehydes
bacteria
Bacteriology
Biological and medical sciences
Culture Media - chemistry
formaldehyde
Formaldehyde - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene Expression Regulation, Bacterial
genes
Gram-negative bacteria
Methanol
Methanol - metabolism
Methylobacterium extorquens
Methylobacterium extorquens - enzymology
Methylobacterium extorquens - genetics
Methylobacterium extorquens - growth & development
Methylobacterium extorquens - metabolism
Microbiology
Miscellaneous
Oxidation
Physiology and Metabolism
promoter regions
Proteins
Sequence Homology, Amino Acid
title XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1
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