Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decrea...
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| Veröffentlicht in: | Journal of plant physiology 2011-11, Vol.168 (17), p.2048-2055 |
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| creator | Okuma, Eiji Jahan, Md. Sarwar Munemasa, Shintaro Hossain, Mohammad Anowar Muroyama, Daichi Islam, Mohammad Mahbub Ogawa, Ken’ichi Watanabe-Sugimoto, Megumi Nakamura, Yoshimasa Shimoishi, Yasuaki Mori, Izumi C. Murata, Yoshiyuki |
| description | We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an
Arabidopsis mutant,
cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the
cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in
cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca
2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca
2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca
2+ oscillation in ABA signal pathway of
Arabidopsis guard cells. |
| doi_str_mv | 10.1016/j.jplph.2011.06.002 |
| format | Article |
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Arabidopsis mutant,
cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the
cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in
cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca
2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca
2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca
2+ oscillation in ABA signal pathway of
Arabidopsis guard cells.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2011.06.002</identifier><identifier>PMID: 21764168</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Abscisic acid ; Abscisic Acid - pharmacology ; Activation ; Arabidopsis - drug effects ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - physiology ; Biological and medical sciences ; Calcium - metabolism ; Calcium Signaling - drug effects ; Cell Membrane - metabolism ; Channels ; Control ; Cytosol - metabolism ; Cytosolic calcium ; Dinitrochlorobenzene - metabolism ; Esters ; Fundamental and applied biological sciences. Psychology ; Genetic Complementation Test ; Glutamate-Cysteine Ligase - genetics ; Glutamate-Cysteine Ligase - metabolism ; Glutathione ; Glutathione - analogs & derivatives ; Glutathione - metabolism ; Glutathione - pharmacology ; Guards ; Mutation ; Mutations ; Oscillations ; Plant physiology and development ; Plant Stomata - drug effects ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Signal Transduction - drug effects ; Stomatal closure</subject><ispartof>Journal of plant physiology, 2011-11, Vol.168 (17), p.2048-2055</ispartof><rights>2011 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-878e97b68bade4973a6402144081ca8606e2415270f565c0b883f60b9b84d0633</citedby><cites>FETCH-LOGICAL-c487t-878e97b68bade4973a6402144081ca8606e2415270f565c0b883f60b9b84d0633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0176161711002768$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24699331$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21764168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okuma, Eiji</creatorcontrib><creatorcontrib>Jahan, Md. Sarwar</creatorcontrib><creatorcontrib>Munemasa, Shintaro</creatorcontrib><creatorcontrib>Hossain, Mohammad Anowar</creatorcontrib><creatorcontrib>Muroyama, Daichi</creatorcontrib><creatorcontrib>Islam, Mohammad Mahbub</creatorcontrib><creatorcontrib>Ogawa, Ken’ichi</creatorcontrib><creatorcontrib>Watanabe-Sugimoto, Megumi</creatorcontrib><creatorcontrib>Nakamura, Yoshimasa</creatorcontrib><creatorcontrib>Shimoishi, Yasuaki</creatorcontrib><creatorcontrib>Mori, Izumi C.</creatorcontrib><creatorcontrib>Murata, Yoshiyuki</creatorcontrib><title>Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an
Arabidopsis mutant,
cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the
cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in
cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca
2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca
2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca
2+ oscillation in ABA signal pathway of
Arabidopsis guard cells.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Activation</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Channels</subject><subject>Control</subject><subject>Cytosol - metabolism</subject><subject>Cytosolic calcium</subject><subject>Dinitrochlorobenzene - metabolism</subject><subject>Esters</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Complementation Test</subject><subject>Glutamate-Cysteine Ligase - genetics</subject><subject>Glutamate-Cysteine Ligase - metabolism</subject><subject>Glutathione</subject><subject>Glutathione - analogs & derivatives</subject><subject>Glutathione - metabolism</subject><subject>Glutathione - pharmacology</subject><subject>Guards</subject><subject>Mutation</subject><subject>Mutations</subject><subject>Oscillations</subject><subject>Plant physiology and development</subject><subject>Plant Stomata - drug effects</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Stomatal closure</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFvFCEUx4nR2G31E5gYLkYvM_IGBpiDh6bR2qRpL3ryQIB5s2UzO4ww06TfXuqu9tYT8Pj94eX3CHkHrAYG8vOu3s3jfFc3DKBmsmaseUE2IEFXwBv9kmwYKFmVgjohpznvWDm3mr8mJ025ECD1hvy6wa1dwj3ShNt1LNs40ThQ67IPOXhqfeirMPWrx57mJe7tYkfqx5jXhNQ90O24Lna5KzmkYaLnybrQxzmH_Ia8GuyY8e1xPSM_v339cfG9ur69vLo4v6680GqptNLYKSe1sz2KTnErBWtACKbBWy2ZxEZA2yg2tLL1zGnNB8lc57TomeT8jHw8vDun-HvFvJh9yB7H0U4Y12x0pzRwgLaQn54lQSrgvO1UV1B-QH2KOScczJzC3qYHA8w8-jc789e_efRvmDTFf0m9P36wuj32_zP_hBfgwxGw2dtxSHYqnp84IbuOcyjclwOHRdx9wGTKPHAqQwgJ_WL6GJ5t5A86j6Oi</recordid><startdate>20111115</startdate><enddate>20111115</enddate><creator>Okuma, Eiji</creator><creator>Jahan, Md. Sarwar</creator><creator>Munemasa, Shintaro</creator><creator>Hossain, Mohammad Anowar</creator><creator>Muroyama, Daichi</creator><creator>Islam, Mohammad Mahbub</creator><creator>Ogawa, Ken’ichi</creator><creator>Watanabe-Sugimoto, Megumi</creator><creator>Nakamura, Yoshimasa</creator><creator>Shimoishi, Yasuaki</creator><creator>Mori, Izumi C.</creator><creator>Murata, Yoshiyuki</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20111115</creationdate><title>Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis</title><author>Okuma, Eiji ; Jahan, Md. Sarwar ; Munemasa, Shintaro ; Hossain, Mohammad Anowar ; Muroyama, Daichi ; Islam, Mohammad Mahbub ; Ogawa, Ken’ichi ; Watanabe-Sugimoto, Megumi ; Nakamura, Yoshimasa ; Shimoishi, Yasuaki ; Mori, Izumi C. ; Murata, Yoshiyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-878e97b68bade4973a6402144081ca8606e2415270f565c0b883f60b9b84d0633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Activation</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Channels</topic><topic>Control</topic><topic>Cytosol - metabolism</topic><topic>Cytosolic calcium</topic><topic>Dinitrochlorobenzene - metabolism</topic><topic>Esters</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Complementation Test</topic><topic>Glutamate-Cysteine Ligase - genetics</topic><topic>Glutamate-Cysteine Ligase - metabolism</topic><topic>Glutathione</topic><topic>Glutathione - analogs & derivatives</topic><topic>Glutathione - metabolism</topic><topic>Glutathione - pharmacology</topic><topic>Guards</topic><topic>Mutation</topic><topic>Mutations</topic><topic>Oscillations</topic><topic>Plant physiology and development</topic><topic>Plant Stomata - drug effects</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Stomatal closure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okuma, Eiji</creatorcontrib><creatorcontrib>Jahan, Md. Sarwar</creatorcontrib><creatorcontrib>Munemasa, Shintaro</creatorcontrib><creatorcontrib>Hossain, Mohammad Anowar</creatorcontrib><creatorcontrib>Muroyama, Daichi</creatorcontrib><creatorcontrib>Islam, Mohammad Mahbub</creatorcontrib><creatorcontrib>Ogawa, Ken’ichi</creatorcontrib><creatorcontrib>Watanabe-Sugimoto, Megumi</creatorcontrib><creatorcontrib>Nakamura, Yoshimasa</creatorcontrib><creatorcontrib>Shimoishi, Yasuaki</creatorcontrib><creatorcontrib>Mori, Izumi C.</creatorcontrib><creatorcontrib>Murata, Yoshiyuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okuma, Eiji</au><au>Jahan, Md. Sarwar</au><au>Munemasa, Shintaro</au><au>Hossain, Mohammad Anowar</au><au>Muroyama, Daichi</au><au>Islam, Mohammad Mahbub</au><au>Ogawa, Ken’ichi</au><au>Watanabe-Sugimoto, Megumi</au><au>Nakamura, Yoshimasa</au><au>Shimoishi, Yasuaki</au><au>Mori, Izumi C.</au><au>Murata, Yoshiyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2011-11-15</date><risdate>2011</risdate><volume>168</volume><issue>17</issue><spage>2048</spage><epage>2055</epage><pages>2048-2055</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an
Arabidopsis mutant,
cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the
cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in
cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca
2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca
2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca
2+ oscillation in ABA signal pathway of
Arabidopsis guard cells.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>21764168</pmid><doi>10.1016/j.jplph.2011.06.002</doi><tpages>8</tpages></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - pharmacology Activation Arabidopsis - drug effects Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - physiology Biological and medical sciences Calcium - metabolism Calcium Signaling - drug effects Cell Membrane - metabolism Channels Control Cytosol - metabolism Cytosolic calcium Dinitrochlorobenzene - metabolism Esters Fundamental and applied biological sciences. Psychology Genetic Complementation Test Glutamate-Cysteine Ligase - genetics Glutamate-Cysteine Ligase - metabolism Glutathione Glutathione - analogs & derivatives Glutathione - metabolism Glutathione - pharmacology Guards Mutation Mutations Oscillations Plant physiology and development Plant Stomata - drug effects Reactive oxygen species Reactive Oxygen Species - metabolism Signal Transduction - drug effects Stomatal closure |
| title | Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis |
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