Partial dehydration and cryopreservation of Citrus seeds
BACKGROUND: Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment o...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2011-11, Vol.91 (14), p.2544-2550 |
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| creator | Graiver, Natalia Califano, Alicia Zaritzky, Noemí |
| description | BACKGROUND: Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN.
RESULTS: To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms.
CONCLUSIONS: The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.4427 |
| format | Article |
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RESULTS: To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms.
CONCLUSIONS: The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>ISSN: 1097-0010</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.4427</identifier><identifier>PMID: 21538368</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Agriculture - methods ; Biological and medical sciences ; Calorimetry, Differential Scanning ; Citrus - chemistry ; Citrus - growth & development ; Citrus fruits ; Citrus paradisi - chemistry ; Citrus paradisi - growth & development ; Citrus seed ; Citrus sinensis - chemistry ; Citrus sinensis - growth & development ; Cotyledon - chemistry ; Cotyledon - growth & development ; Cryopreservation ; Dehydration ; Desiccation ; Differential scanning calorimetry ; Diffusion ; DSC ; Food engineering ; Food industries ; Food preservation ; Food science ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; Germination ; Hydration ; Lipids ; Lipids - analysis ; Lipids - chemistry ; Mandarins ; Models, Biological ; Moisture content ; Phase Transition ; Plant Extracts - chemistry ; Relative humidity ; Seeds ; Seeds - chemistry ; Seeds - growth & development ; Species Specificity ; unfrozen water ; Water - analysis ; water activity</subject><ispartof>Journal of the science of food and agriculture, 2011-11, Vol.91 (14), p.2544-2550</ispartof><rights>Copyright © 2011 Society of Chemical Industry</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Society of Chemical Industry.</rights><rights>Copyright John Wiley and Sons, Limited Nov 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4527-da2bde88570aaa9c2c4bb182eb66c227a4376b135429536d4fff785c0a0f8dc33</citedby><cites>FETCH-LOGICAL-c4527-da2bde88570aaa9c2c4bb182eb66c227a4376b135429536d4fff785c0a0f8dc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.4427$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.4427$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,1416,23928,23929,25138,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24607148$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21538368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graiver, Natalia</creatorcontrib><creatorcontrib>Califano, Alicia</creatorcontrib><creatorcontrib>Zaritzky, Noemí</creatorcontrib><title>Partial dehydration and cryopreservation of Citrus seeds</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>BACKGROUND: Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN.
RESULTS: To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms.
CONCLUSIONS: The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry</description><subject>Agriculture - methods</subject><subject>Biological and medical sciences</subject><subject>Calorimetry, Differential Scanning</subject><subject>Citrus - chemistry</subject><subject>Citrus - growth & development</subject><subject>Citrus fruits</subject><subject>Citrus paradisi - chemistry</subject><subject>Citrus paradisi - growth & development</subject><subject>Citrus seed</subject><subject>Citrus sinensis - chemistry</subject><subject>Citrus sinensis - growth & development</subject><subject>Cotyledon - chemistry</subject><subject>Cotyledon - growth & development</subject><subject>Cryopreservation</subject><subject>Dehydration</subject><subject>Desiccation</subject><subject>Differential scanning calorimetry</subject><subject>Diffusion</subject><subject>DSC</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Food preservation</subject><subject>Food science</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Germination</subject><subject>Hydration</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Lipids - chemistry</subject><subject>Mandarins</subject><subject>Models, Biological</subject><subject>Moisture content</subject><subject>Phase Transition</subject><subject>Plant Extracts - chemistry</subject><subject>Relative humidity</subject><subject>Seeds</subject><subject>Seeds - chemistry</subject><subject>Seeds - growth & development</subject><subject>Species Specificity</subject><subject>unfrozen water</subject><subject>Water - analysis</subject><subject>water activity</subject><issn>0022-5142</issn><issn>1097-0010</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MtO3DAUBmALFcGUdsELVFElBCwCvsV2ljACWoR6ESCW1okvaqaZZLCTwrx9HWVKpUp0Zcn-zn_kH6F9gk8IxvR0ET2ccE7lFpoRXMocY4LfoFl6o3lBON1Fb2NcYIzLUogdtEtJwRQTaobUNwh9DU1m3Y-1DdDXXZtBazMT1t0quOjCr-my89m87sMQs-icje_Qtocmuvebcw_dX17czT_lN1-vPs_PbnLDCypzC7SyTqlCYgAoDTW8qoiirhLCUCqBMykqwgpOy4IJy733UhUGA_bKGsb20OGUuwrd4-Bir5d1NK5poHXdELUqpSKUCJ7k0X8lSW0oRTgb6cd_6KIbQpv-kfKUKnHJSELHEzKhizE4r1ehXkJYpyQ99q7H3vXYe7IfNoFDtXT2Rf4pOoGDDYBooPEBWlPHv44LLAkf3enknurGrV_fqK9vL882q_Npoo69e36ZgPBTC8lkoR--XOnb73cP4nx-rjn7DRp6p0o</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Graiver, Natalia</creator><creator>Califano, Alicia</creator><creator>Zaritzky, Noemí</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>John Wiley and Sons, Limited</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>Partial dehydration and cryopreservation of Citrus seeds</title><author>Graiver, Natalia ; Califano, Alicia ; Zaritzky, Noemí</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4527-da2bde88570aaa9c2c4bb182eb66c227a4376b135429536d4fff785c0a0f8dc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agriculture - methods</topic><topic>Biological and medical sciences</topic><topic>Calorimetry, Differential Scanning</topic><topic>Citrus - chemistry</topic><topic>Citrus - growth & development</topic><topic>Citrus fruits</topic><topic>Citrus paradisi - chemistry</topic><topic>Citrus paradisi - growth & development</topic><topic>Citrus seed</topic><topic>Citrus sinensis - chemistry</topic><topic>Citrus sinensis - growth & development</topic><topic>Cotyledon - chemistry</topic><topic>Cotyledon - growth & development</topic><topic>Cryopreservation</topic><topic>Dehydration</topic><topic>Desiccation</topic><topic>Differential scanning calorimetry</topic><topic>Diffusion</topic><topic>DSC</topic><topic>Food engineering</topic><topic>Food industries</topic><topic>Food preservation</topic><topic>Food science</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Germination</topic><topic>Hydration</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Lipids - chemistry</topic><topic>Mandarins</topic><topic>Models, Biological</topic><topic>Moisture content</topic><topic>Phase Transition</topic><topic>Plant Extracts - chemistry</topic><topic>Relative humidity</topic><topic>Seeds</topic><topic>Seeds - chemistry</topic><topic>Seeds - growth & development</topic><topic>Species Specificity</topic><topic>unfrozen water</topic><topic>Water - analysis</topic><topic>water activity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graiver, Natalia</creatorcontrib><creatorcontrib>Califano, Alicia</creatorcontrib><creatorcontrib>Zaritzky, Noemí</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graiver, Natalia</au><au>Califano, Alicia</au><au>Zaritzky, Noemí</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Partial dehydration and cryopreservation of Citrus seeds</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2011-11</date><risdate>2011</risdate><volume>91</volume><issue>14</issue><spage>2544</spage><epage>2550</epage><pages>2544-2550</pages><issn>0022-5142</issn><issn>1097-0010</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>BACKGROUND: Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN.
RESULTS: To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms.
CONCLUSIONS: The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>21538368</pmid><doi>10.1002/jsfa.4427</doi><tpages>7</tpages></addata></record> |
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| subjects | Agriculture - methods Biological and medical sciences Calorimetry, Differential Scanning Citrus - chemistry Citrus - growth & development Citrus fruits Citrus paradisi - chemistry Citrus paradisi - growth & development Citrus seed Citrus sinensis - chemistry Citrus sinensis - growth & development Cotyledon - chemistry Cotyledon - growth & development Cryopreservation Dehydration Desiccation Differential scanning calorimetry Diffusion DSC Food engineering Food industries Food preservation Food science Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects Germination Hydration Lipids Lipids - analysis Lipids - chemistry Mandarins Models, Biological Moisture content Phase Transition Plant Extracts - chemistry Relative humidity Seeds Seeds - chemistry Seeds - growth & development Species Specificity unfrozen water Water - analysis water activity |
| title | Partial dehydration and cryopreservation of Citrus seeds |
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