Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high-performance liquid chromatography
► Liquid–liquid microextraction-high performance liquid chromatography method for opium alkaloids. ► Determination of morphine, codeine, papaverine, noscapine and thebaine in urine samples. ► Study of optimum experimental conditions for extraction process. ► Linear calibration over 0.50–500 μg L −1...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (28), p.2978-2983 |
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| container_issue | 28 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 879 |
| creator | Shamsipur, Mojtaba Fattahi, Nazir |
| description | ► Liquid–liquid microextraction-high performance liquid chromatography method for opium alkaloids. ► Determination of morphine, codeine, papaverine, noscapine and thebaine in urine samples. ► Study of optimum experimental conditions for extraction process. ► Linear calibration over 0.50–500
μg
L
−1 and limit of detections in the ranges 0.2–10
μg
L
−1.
A simple, rapid and sensitive method based on dispersive liquid–liquid microextraction (DLLME) combined with high-performance liquid chromatography–ultraviolet detection (HPLC-UV) was used to determine opium alkaloids in urine samples. Some effective parameters on extraction were studied and optimized. Under the optimum conditions, enrichment factors and recoveries for different opiates are in the range of 63.0–104.5 and 31.5–52.2%, respectively. The calibration graphs are linear in the range of 0.50–500
μg
L
−1 and limit of detections (LODs) are in the range of 0.2–10
μg
L
−1. The relative standard deviations (RSDs) for 200
μg
L
−1 of morphine, codeine and thebaine, 5.0
μg
L
−1 of papaverine and 10.0
μg
L
−1 of noscapine in diluted urine sample are in the range of 2.8–6.1% (
n
=
7). The relative recoveries of urine samples spiked with alkaloids are 84.3–106.0%. The obtained results show that DLLME combined with HPLC-UV is a fast and simple method for the determination of opium alkaloids in urine samples. |
| doi_str_mv | 10.1016/j.jchromb.2011.08.033 |
| format | Article |
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μg
L
−1 and limit of detections in the ranges 0.2–10
μg
L
−1.
A simple, rapid and sensitive method based on dispersive liquid–liquid microextraction (DLLME) combined with high-performance liquid chromatography–ultraviolet detection (HPLC-UV) was used to determine opium alkaloids in urine samples. Some effective parameters on extraction were studied and optimized. Under the optimum conditions, enrichment factors and recoveries for different opiates are in the range of 63.0–104.5 and 31.5–52.2%, respectively. The calibration graphs are linear in the range of 0.50–500
μg
L
−1 and limit of detections (LODs) are in the range of 0.2–10
μg
L
−1. The relative standard deviations (RSDs) for 200
μg
L
−1 of morphine, codeine and thebaine, 5.0
μg
L
−1 of papaverine and 10.0
μg
L
−1 of noscapine in diluted urine sample are in the range of 2.8–6.1% (
n
=
7). The relative recoveries of urine samples spiked with alkaloids are 84.3–106.0%. The obtained results show that DLLME combined with HPLC-UV is a fast and simple method for the determination of opium alkaloids in urine samples.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.08.033</identifier><identifier>PMID: 21925978</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Chromatography, High Pressure Liquid - methods ; codeine ; Dispersive liquid–liquid microextraction ; High-performance liquid chromatography ; Humans ; Liquid Phase Microextraction - methods ; liquid-phase microextraction ; morphine ; Opiate Alkaloids - isolation & purification ; Opiate Alkaloids - urine ; Opium alkaloids ; papaverine ; Sensitivity and Specificity ; thebaine ; urine ; Urine analysis</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (28), p.2978-2983</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-dba9ae01b7cdfa0f6c43a7684f460cf3ad7f4537e5e1b61795050aea49b218073</citedby><cites>FETCH-LOGICAL-c388t-dba9ae01b7cdfa0f6c43a7684f460cf3ad7f4537e5e1b61795050aea49b218073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023211005599$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21925978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shamsipur, Mojtaba</creatorcontrib><creatorcontrib>Fattahi, Nazir</creatorcontrib><title>Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high-performance liquid chromatography</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>► Liquid–liquid microextraction-high performance liquid chromatography method for opium alkaloids. ► Determination of morphine, codeine, papaverine, noscapine and thebaine in urine samples. ► Study of optimum experimental conditions for extraction process. ► Linear calibration over 0.50–500
μg
L
−1 and limit of detections in the ranges 0.2–10
μg
L
−1.
A simple, rapid and sensitive method based on dispersive liquid–liquid microextraction (DLLME) combined with high-performance liquid chromatography–ultraviolet detection (HPLC-UV) was used to determine opium alkaloids in urine samples. Some effective parameters on extraction were studied and optimized. Under the optimum conditions, enrichment factors and recoveries for different opiates are in the range of 63.0–104.5 and 31.5–52.2%, respectively. The calibration graphs are linear in the range of 0.50–500
μg
L
−1 and limit of detections (LODs) are in the range of 0.2–10
μg
L
−1. The relative standard deviations (RSDs) for 200
μg
L
−1 of morphine, codeine and thebaine, 5.0
μg
L
−1 of papaverine and 10.0
μg
L
−1 of noscapine in diluted urine sample are in the range of 2.8–6.1% (
n
=
7). The relative recoveries of urine samples spiked with alkaloids are 84.3–106.0%. The obtained results show that DLLME combined with HPLC-UV is a fast and simple method for the determination of opium alkaloids in urine samples.</description><subject>Chromatography, High Pressure Liquid - methods</subject><subject>codeine</subject><subject>Dispersive liquid–liquid microextraction</subject><subject>High-performance liquid chromatography</subject><subject>Humans</subject><subject>Liquid Phase Microextraction - methods</subject><subject>liquid-phase microextraction</subject><subject>morphine</subject><subject>Opiate Alkaloids - isolation & purification</subject><subject>Opiate Alkaloids - urine</subject><subject>Opium alkaloids</subject><subject>papaverine</subject><subject>Sensitivity and Specificity</subject><subject>thebaine</subject><subject>urine</subject><subject>Urine analysis</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1u1TAUhSMEoqWwBMAzRgl2nMTOCKGqBaRKDKASM-vGvn7PjyRO7aTlzboHtsDKWAlu8wpDRr6yvnN_zsmyl4wWjLLm7a7Y6W3wQ1eUlLGCyoJy_ig7ZlLwnIvm2-NU14LmtOTlUfYsxh2lTFDBn2ZHJWvLuhXyOPt19mMOoGfnRwKjIQZnDIMb4f7HW-IntwwE-u_Qe2cicSNZghuRRBimHiNZohs3xLg4YYjuGknvrhZnft_-XAsyOB08_htjfd_7GzSk25Ot22zzJLQ-DDDqBzG5Pw1mvwkwbffPsycW-ogvDu9Jdnl-9vX0Y37x-cOn0_cXueZSzrnpoAWkrBPaWKC20RUH0cjKVg3VloMRtqq5wBpZ1zDR1rSmgFC1XclkcuYke7P2nYK_WjDOanBRY9_DiH6JSraN5Mltmch6JdNpMQa0agpugLBXjKq7fNROHfJRd_koKlXKJ-leHSYs3YDmr-ohkAS8XgELXsEmuKguv6QODaW0qeumTcS7lcDkxLXDoKJ2mLwzLqCelfHuP0v8AUNvtHw</recordid><startdate>20111015</startdate><enddate>20111015</enddate><creator>Shamsipur, Mojtaba</creator><creator>Fattahi, Nazir</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111015</creationdate><title>Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high-performance liquid chromatography</title><author>Shamsipur, Mojtaba ; Fattahi, Nazir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-dba9ae01b7cdfa0f6c43a7684f460cf3ad7f4537e5e1b61795050aea49b218073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Chromatography, High Pressure Liquid - methods</topic><topic>codeine</topic><topic>Dispersive liquid–liquid microextraction</topic><topic>High-performance liquid chromatography</topic><topic>Humans</topic><topic>Liquid Phase Microextraction - methods</topic><topic>liquid-phase microextraction</topic><topic>morphine</topic><topic>Opiate Alkaloids - isolation & purification</topic><topic>Opiate Alkaloids - urine</topic><topic>Opium alkaloids</topic><topic>papaverine</topic><topic>Sensitivity and Specificity</topic><topic>thebaine</topic><topic>urine</topic><topic>Urine analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shamsipur, Mojtaba</creatorcontrib><creatorcontrib>Fattahi, Nazir</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shamsipur, Mojtaba</au><au>Fattahi, Nazir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high-performance liquid chromatography</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-10-15</date><risdate>2011</risdate><volume>879</volume><issue>28</issue><spage>2978</spage><epage>2983</epage><pages>2978-2983</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>► Liquid–liquid microextraction-high performance liquid chromatography method for opium alkaloids. ► Determination of morphine, codeine, papaverine, noscapine and thebaine in urine samples. ► Study of optimum experimental conditions for extraction process. ► Linear calibration over 0.50–500
μg
L
−1 and limit of detections in the ranges 0.2–10
μg
L
−1.
A simple, rapid and sensitive method based on dispersive liquid–liquid microextraction (DLLME) combined with high-performance liquid chromatography–ultraviolet detection (HPLC-UV) was used to determine opium alkaloids in urine samples. Some effective parameters on extraction were studied and optimized. Under the optimum conditions, enrichment factors and recoveries for different opiates are in the range of 63.0–104.5 and 31.5–52.2%, respectively. The calibration graphs are linear in the range of 0.50–500
μg
L
−1 and limit of detections (LODs) are in the range of 0.2–10
μg
L
−1. The relative standard deviations (RSDs) for 200
μg
L
−1 of morphine, codeine and thebaine, 5.0
μg
L
−1 of papaverine and 10.0
μg
L
−1 of noscapine in diluted urine sample are in the range of 2.8–6.1% (
n
=
7). The relative recoveries of urine samples spiked with alkaloids are 84.3–106.0%. The obtained results show that DLLME combined with HPLC-UV is a fast and simple method for the determination of opium alkaloids in urine samples.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21925978</pmid><doi>10.1016/j.jchromb.2011.08.033</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (28), p.2978-2983 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Chromatography, High Pressure Liquid - methods codeine Dispersive liquid–liquid microextraction High-performance liquid chromatography Humans Liquid Phase Microextraction - methods liquid-phase microextraction morphine Opiate Alkaloids - isolation & purification Opiate Alkaloids - urine Opium alkaloids papaverine Sensitivity and Specificity thebaine urine Urine analysis |
| title | Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high-performance liquid chromatography |
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