Engineered Streptavidin Monomer and Dimer with Improved Stability and Function

Although streptavidin’s high affinity for biotin has made it a widely used and studied binding protein and labeling tool, its tetrameric structure may interfere with some assays. A streptavidin mutant with a simpler quaternary structure would demonstrate a molecular-level understanding of its struct...

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Veröffentlicht in:	Biochemistry (Easton) 2011-10, Vol.50 (40), p.8682-8691
Hauptverfasser:	Lim, Kok Hong, Huang, Heng, Pralle, Arnd, Park, Sheldon
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biotin - metabolism Dimerization Kinetics Molecular Sequence Data Mutation Protein Binding Protein Engineering Protein Stability Streptavidin - chemistry Streptavidin - genetics Streptavidin - metabolism Streptomyces - chemistry Streptomyces - genetics Streptomyces - metabolism
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