Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella
Salmonella species are ubiquitous human pathogens which pose a dangerous threat to the elderly and children worldwide. In this study, to develop a more efficient assay procedure for the rapid detection of Salmonella Typhimurium, an immunochromatographic strip assay was developed using immunoliposome...
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| description | Salmonella
species are ubiquitous human pathogens which pose a dangerous threat to the elderly and children worldwide. In this study, to develop a more efficient assay procedure for the rapid detection of
Salmonella
Typhimurium, an immunochromatographic strip assay was developed using immunoliposome (anti-
Salmonella
IgG-tagged) encapsulated with sulforhodamine B (SRB). The detection sensitivity of the developed immunochromatographic assay was compared with a commercial immunochromatographic test strip using colloidal gold nanoparticles. The liposomes were prepared through a reverse-phase evaporation method by using a lipid and phospholipid mixture and SRB, a fluorescence dye, which was encapsulated in the phospholipid bilayer. Furthermore, the outer surface of the SRB-encapsulated liposome was conjugated with antibody (affinity-purified polyclonal goat anti-
Salmonella
IgG) to form an immunoliposome (size, 223 nm), used as the analytical reagent in the developed immunoassay. For this strip assay, a plastic-backed nitrocellulose strip was immobilized with two antibody zones. The lower zone of the strip referred to
Salmonella
antigen capture zone (test line), while the other zone served as a positive control (control line). The lower zone was coated with affinity-purified polyclonal goat anti-
Salmonella
IgG, while the upper zone was coated with rabbit anti-goat IgG. During capillary migration of the wicking solution (diluted liposome and
Salmonella
culture, each 50 μl),
Salmonella
was captured with surface-bound immunoliposomes at the antigen capture zone, while the unbound liposomes migrated upward and bound to another zone. The color density of the antigen capture zone was directly proportional to the amount of
S.
Typhimurium in the test sample. As a result, the detection limit of the immunochromatographic strip assay developed in this study against
S
. Typhimurium was found to be 10
2
CFU/ml, which was significantly higher than the detection limit (10
7
CFU/ml) of the commercial immunochromatographic test strip assay.
Figa
The concept of immunochromatographic strip assay |
| doi_str_mv | 10.1007/s00216-011-5327-2 |
| format | Article |
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species are ubiquitous human pathogens which pose a dangerous threat to the elderly and children worldwide. In this study, to develop a more efficient assay procedure for the rapid detection of
Salmonella
Typhimurium, an immunochromatographic strip assay was developed using immunoliposome (anti-
Salmonella
IgG-tagged) encapsulated with sulforhodamine B (SRB). The detection sensitivity of the developed immunochromatographic assay was compared with a commercial immunochromatographic test strip using colloidal gold nanoparticles. The liposomes were prepared through a reverse-phase evaporation method by using a lipid and phospholipid mixture and SRB, a fluorescence dye, which was encapsulated in the phospholipid bilayer. Furthermore, the outer surface of the SRB-encapsulated liposome was conjugated with antibody (affinity-purified polyclonal goat anti-
Salmonella
IgG) to form an immunoliposome (size, 223 nm), used as the analytical reagent in the developed immunoassay. For this strip assay, a plastic-backed nitrocellulose strip was immobilized with two antibody zones. The lower zone of the strip referred to
Salmonella
antigen capture zone (test line), while the other zone served as a positive control (control line). The lower zone was coated with affinity-purified polyclonal goat anti-
Salmonella
IgG, while the upper zone was coated with rabbit anti-goat IgG. During capillary migration of the wicking solution (diluted liposome and
Salmonella
culture, each 50 μl),
Salmonella
was captured with surface-bound immunoliposomes at the antigen capture zone, while the unbound liposomes migrated upward and bound to another zone. The color density of the antigen capture zone was directly proportional to the amount of
S.
Typhimurium in the test sample. As a result, the detection limit of the immunochromatographic strip assay developed in this study against
S
. Typhimurium was found to be 10
2
CFU/ml, which was significantly higher than the detection limit (10
7
CFU/ml) of the commercial immunochromatographic test strip assay.
Figa
The concept of immunochromatographic strip assay</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-011-5327-2</identifier><identifier>PMID: 21863217</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Antigens ; Assaying ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatographic methods and physical methods associated with chromatography ; Exact sciences and technology ; Fluorescence ; Food Science ; Goats ; Humans ; Immunoassay - economics ; Immunoassay - methods ; Immunochromatography - economics ; Immunochromatography - methods ; Immunoglobulin G ; Laboratory Medicine ; Liposomes ; Liposomes - immunology ; Mathematical analysis ; Miscellaneous ; Monitoring/Environmental Analysis ; Original Paper ; Other chromatographic methods ; Phospholipids ; Salmonella ; Salmonella food poisoning ; Salmonella Infections - diagnosis ; Salmonella Infections - immunology ; Salmonella typhimurium ; Salmonella typhimurium - immunology ; Salmonella typhimurium - isolation & purification ; Sensitivity and Specificity ; Spectrometric and optical methods ; Strip ; Time Factors</subject><ispartof>Analytical and bioanalytical chemistry, 2011-11, Vol.401 (8), p.2581-2590</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c638t-180cc14c8401f5f1ab45d962ced4259cc506d61f9b130ef763196cbb516492713</citedby><cites>FETCH-LOGICAL-c638t-180cc14c8401f5f1ab45d962ced4259cc506d61f9b130ef763196cbb516492713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-011-5327-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-011-5327-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24635880$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21863217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shukla, Shruti</creatorcontrib><creatorcontrib>Leem, Hyerim</creatorcontrib><creatorcontrib>Kim, Myunghee</creatorcontrib><title>Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Salmonella
species are ubiquitous human pathogens which pose a dangerous threat to the elderly and children worldwide. In this study, to develop a more efficient assay procedure for the rapid detection of
Salmonella
Typhimurium, an immunochromatographic strip assay was developed using immunoliposome (anti-
Salmonella
IgG-tagged) encapsulated with sulforhodamine B (SRB). The detection sensitivity of the developed immunochromatographic assay was compared with a commercial immunochromatographic test strip using colloidal gold nanoparticles. The liposomes were prepared through a reverse-phase evaporation method by using a lipid and phospholipid mixture and SRB, a fluorescence dye, which was encapsulated in the phospholipid bilayer. Furthermore, the outer surface of the SRB-encapsulated liposome was conjugated with antibody (affinity-purified polyclonal goat anti-
Salmonella
IgG) to form an immunoliposome (size, 223 nm), used as the analytical reagent in the developed immunoassay. For this strip assay, a plastic-backed nitrocellulose strip was immobilized with two antibody zones. The lower zone of the strip referred to
Salmonella
antigen capture zone (test line), while the other zone served as a positive control (control line). The lower zone was coated with affinity-purified polyclonal goat anti-
Salmonella
IgG, while the upper zone was coated with rabbit anti-goat IgG. During capillary migration of the wicking solution (diluted liposome and
Salmonella
culture, each 50 μl),
Salmonella
was captured with surface-bound immunoliposomes at the antigen capture zone, while the unbound liposomes migrated upward and bound to another zone. The color density of the antigen capture zone was directly proportional to the amount of
S.
Typhimurium in the test sample. As a result, the detection limit of the immunochromatographic strip assay developed in this study against
S
. Typhimurium was found to be 10
2
CFU/ml, which was significantly higher than the detection limit (10
7
CFU/ml) of the commercial immunochromatographic test strip assay.
Figa
The concept of immunochromatographic strip assay</description><subject>Analytical Chemistry</subject><subject>Antigens</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>Food Science</subject><subject>Goats</subject><subject>Humans</subject><subject>Immunoassay - economics</subject><subject>Immunoassay - methods</subject><subject>Immunochromatography - economics</subject><subject>Immunochromatography - methods</subject><subject>Immunoglobulin G</subject><subject>Laboratory Medicine</subject><subject>Liposomes</subject><subject>Liposomes - immunology</subject><subject>Mathematical analysis</subject><subject>Miscellaneous</subject><subject>Monitoring/Environmental Analysis</subject><subject>Original Paper</subject><subject>Other chromatographic methods</subject><subject>Phospholipids</subject><subject>Salmonella</subject><subject>Salmonella food poisoning</subject><subject>Salmonella Infections - diagnosis</subject><subject>Salmonella Infections - immunology</subject><subject>Salmonella typhimurium</subject><subject>Salmonella typhimurium - immunology</subject><subject>Salmonella typhimurium - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometric and optical methods</subject><subject>Strip</subject><subject>Time Factors</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl1rFTEQhoMoth79Ad7Igki92ZrJ12YvS60fUPBCvQ7ZbHJOymazJrtC_71Z9lgRpJZcJCTPvDOTeRF6CfgcMG7eZYwJiBoD1JySpiaP0CkIkDURHD--OzNygp7lfIMxcAniKTohIAUl0Jwi_d7-tEOcgh3nKrpKV4OfYo7B1p3Otq98CMsYzSHFoOe4T3o6eFPlOfmp0jnr28rFVM0HW_V2tmb2cVx1vuohxNEOg36Onjg9ZPviuO_Q9w9X3y4_1ddfPn6-vLiujaByrkFiY4AZyTA47kB3jPetIMb2jPDWGI5FL8C1HVBsXSMotMJ0HQfBWtIA3aGzTXdK8cdi86yCz2atYLRxyUoWMdoSTAv59l4SGsYaELTwD0NpS_n_USCc0NLdAwrAooW2kaXJHXq9oXs9WOVHF-ekzYqrC8pkGT-XrFDn_6DK6m3wpszB-XL_VwBsASbFnJN1ako-6HRbcqvVW2rzlireUqu31PoXr45VL12w_V3EbzMV4M0R0NnowSU9Gp__cExQLiUuHNm4XJ7GvU3qJi5pLOa4J_svERji1g</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Shukla, Shruti</creator><creator>Leem, Hyerim</creator><creator>Kim, Myunghee</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7UA</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20111101</creationdate><title>Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella</title><author>Shukla, Shruti ; Leem, Hyerim ; Kim, Myunghee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c638t-180cc14c8401f5f1ab45d962ced4259cc506d61f9b130ef763196cbb516492713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical Chemistry</topic><topic>Antigens</topic><topic>Assaying</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>Food Science</topic><topic>Goats</topic><topic>Humans</topic><topic>Immunoassay - economics</topic><topic>Immunoassay - methods</topic><topic>Immunochromatography - economics</topic><topic>Immunochromatography - methods</topic><topic>Immunoglobulin G</topic><topic>Laboratory Medicine</topic><topic>Liposomes</topic><topic>Liposomes - immunology</topic><topic>Mathematical analysis</topic><topic>Miscellaneous</topic><topic>Monitoring/Environmental Analysis</topic><topic>Original Paper</topic><topic>Other chromatographic methods</topic><topic>Phospholipids</topic><topic>Salmonella</topic><topic>Salmonella food poisoning</topic><topic>Salmonella Infections - diagnosis</topic><topic>Salmonella Infections - immunology</topic><topic>Salmonella typhimurium</topic><topic>Salmonella typhimurium - immunology</topic><topic>Salmonella typhimurium - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometric and optical methods</topic><topic>Strip</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shukla, Shruti</creatorcontrib><creatorcontrib>Leem, Hyerim</creatorcontrib><creatorcontrib>Kim, Myunghee</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shukla, Shruti</au><au>Leem, Hyerim</au><au>Kim, Myunghee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>401</volume><issue>8</issue><spage>2581</spage><epage>2590</epage><pages>2581-2590</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Salmonella
species are ubiquitous human pathogens which pose a dangerous threat to the elderly and children worldwide. In this study, to develop a more efficient assay procedure for the rapid detection of
Salmonella
Typhimurium, an immunochromatographic strip assay was developed using immunoliposome (anti-
Salmonella
IgG-tagged) encapsulated with sulforhodamine B (SRB). The detection sensitivity of the developed immunochromatographic assay was compared with a commercial immunochromatographic test strip using colloidal gold nanoparticles. The liposomes were prepared through a reverse-phase evaporation method by using a lipid and phospholipid mixture and SRB, a fluorescence dye, which was encapsulated in the phospholipid bilayer. Furthermore, the outer surface of the SRB-encapsulated liposome was conjugated with antibody (affinity-purified polyclonal goat anti-
Salmonella
IgG) to form an immunoliposome (size, 223 nm), used as the analytical reagent in the developed immunoassay. For this strip assay, a plastic-backed nitrocellulose strip was immobilized with two antibody zones. The lower zone of the strip referred to
Salmonella
antigen capture zone (test line), while the other zone served as a positive control (control line). The lower zone was coated with affinity-purified polyclonal goat anti-
Salmonella
IgG, while the upper zone was coated with rabbit anti-goat IgG. During capillary migration of the wicking solution (diluted liposome and
Salmonella
culture, each 50 μl),
Salmonella
was captured with surface-bound immunoliposomes at the antigen capture zone, while the unbound liposomes migrated upward and bound to another zone. The color density of the antigen capture zone was directly proportional to the amount of
S.
Typhimurium in the test sample. As a result, the detection limit of the immunochromatographic strip assay developed in this study against
S
. Typhimurium was found to be 10
2
CFU/ml, which was significantly higher than the detection limit (10
7
CFU/ml) of the commercial immunochromatographic test strip assay.
Figa
The concept of immunochromatographic strip assay</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21863217</pmid><doi>10.1007/s00216-011-5327-2</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2011-11, Vol.401 (8), p.2581-2590 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_896239203 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Antigens Assaying Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Fluorescence Food Science Goats Humans Immunoassay - economics Immunoassay - methods Immunochromatography - economics Immunochromatography - methods Immunoglobulin G Laboratory Medicine Liposomes Liposomes - immunology Mathematical analysis Miscellaneous Monitoring/Environmental Analysis Original Paper Other chromatographic methods Phospholipids Salmonella Salmonella food poisoning Salmonella Infections - diagnosis Salmonella Infections - immunology Salmonella typhimurium Salmonella typhimurium - immunology Salmonella typhimurium - isolation & purification Sensitivity and Specificity Spectrometric and optical methods Strip Time Factors |
| title | Development of a liposome-based immunochromatographic strip assay for the detection of Salmonella |
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