Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA in untreated capillary

A technique utilizing CGE-LIF in a bare capillary has been developed and evaluated for the detection of the three different topoisomers (linear, open circle, and supercoiled) of plasmid DNA along with the prospect of the dimer form of the supercoiled isoform. Utilizing the zwitterionic buffer, HEPES...

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Veröffentlicht in:	Electrophoresis 2010-07, Vol.31 (14), p.2436-2441
Hauptverfasser:	Holovics, Heidi J, He, Yan, Lacher, Nathan A, Ruesch, Margaret N
Format:	Artikel
Sprache:	eng
Schlagworte:	Boric Acids Buffers Capillarity CGE DNA DNA - isolation & purification Electrophoresis Electrophoresis, Capillary - methods Feasibility Fluorescence HEPES Lasers Plasmid Plasmid DNA Plasmids - isolation & purification Platforms Walls
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