The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs

The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs

A sensitive and high throughput off-line [micro]Elution 96-well solid-phase extraction (SPE) followed by strong cation exchange (SCX) liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for determination of cefepime has been developed and validated in mouse plasma. Using th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-06, Vol.878 (19), p.1623-1628
Hauptverfasser: Bu, Wei, Sexton, Holly, Fan, Xiaoqing, Torres, Patricia, Houston, Paul, Heyman, Irwin, Liu, Liang
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1628
container_issue 19
container_start_page 1623
container_title Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
container_volume 878
creator Bu, Wei
Sexton, Holly
Fan, Xiaoqing
Torres, Patricia
Houston, Paul
Heyman, Irwin
Liu, Liang
description A sensitive and high throughput off-line [micro]Elution 96-well solid-phase extraction (SPE) followed by strong cation exchange (SCX) liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for determination of cefepime has been developed and validated in mouse plasma. Using the chemical analog, ceftazidime as an internal standard (IS), the linear range of the method for the determination of cefepime in mouse plasma was 4-2048 ng/mL with the lower limit of quantitation level (LLOQ) of 4 ng/mL. The inter- and intra-assay precision and accuracy of the method were below 9.05% and ranged from 95.6 to 113%, respectively, determined by quality control (QC) samples at five concentration levels including LLOQ. After [micro]Elution SPE, 71.1% of cefepime was recovered. The application of the validated assay for the determination of cefepime in mouse pharmacokinetics (PK) samples after intravenous (IV) and subcutaneous (SC) doses was demonstrated.
doi_str_mv 10.1016/j.jchromb.2010.03.045
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_896188877</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>896188877</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_8961888773</originalsourceid><addsrcrecordid>eNqNjs9OwzAMxnMAifHnEZB849QuXdnanachDnAaBySEpqx1G09JHJp0Y4_JGxFNPACSJVv29_38CXFfyLyQxWK6z_eNHtju8plMO1nm8nF-ISbFvJKZnJWzK3Edwl7KopJVORE_bxrB8QENBHSBIh0QlGtBU68hJtTYaz9GaDHiYMmpSOyAO2iwQ08WgRxYHgOCNypYBbsTbFbv2ctq-rpJBRaj5hY6NoaP5Ppk7jJDDuHDUjPw59qMZ-hykR3RpCBsqM28VomJ33FQzfkcGcLoPQ8RwilETOaUNNKOOKbRD9wPyoZbcdkpE_Dur9-Ih6f12-o5S4KvEUPcWgpN-qMcptjberko6rquqvL_yl_jYXng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>896188877</pqid></control><display><type>article</type><title>The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs</title><source>Access via ScienceDirect (Elsevier)</source><creator>Bu, Wei ; Sexton, Holly ; Fan, Xiaoqing ; Torres, Patricia ; Houston, Paul ; Heyman, Irwin ; Liu, Liang</creator><creatorcontrib>Bu, Wei ; Sexton, Holly ; Fan, Xiaoqing ; Torres, Patricia ; Houston, Paul ; Heyman, Irwin ; Liu, Liang</creatorcontrib><description>A sensitive and high throughput off-line [micro]Elution 96-well solid-phase extraction (SPE) followed by strong cation exchange (SCX) liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for determination of cefepime has been developed and validated in mouse plasma. Using the chemical analog, ceftazidime as an internal standard (IS), the linear range of the method for the determination of cefepime in mouse plasma was 4-2048 ng/mL with the lower limit of quantitation level (LLOQ) of 4 ng/mL. The inter- and intra-assay precision and accuracy of the method were below 9.05% and ranged from 95.6 to 113%, respectively, determined by quality control (QC) samples at five concentration levels including LLOQ. After [micro]Elution SPE, 71.1% of cefepime was recovered. The application of the validated assay for the determination of cefepime in mouse pharmacokinetics (PK) samples after intravenous (IV) and subcutaneous (SC) doses was demonstrated.</description><identifier>ISSN: 1570-0232</identifier><identifier>DOI: 10.1016/j.jchromb.2010.03.045</identifier><language>eng</language><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-06, Vol.878 (19), p.1623-1628</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Bu, Wei</creatorcontrib><creatorcontrib>Sexton, Holly</creatorcontrib><creatorcontrib>Fan, Xiaoqing</creatorcontrib><creatorcontrib>Torres, Patricia</creatorcontrib><creatorcontrib>Houston, Paul</creatorcontrib><creatorcontrib>Heyman, Irwin</creatorcontrib><creatorcontrib>Liu, Liang</creatorcontrib><title>The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><description>A sensitive and high throughput off-line [micro]Elution 96-well solid-phase extraction (SPE) followed by strong cation exchange (SCX) liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for determination of cefepime has been developed and validated in mouse plasma. Using the chemical analog, ceftazidime as an internal standard (IS), the linear range of the method for the determination of cefepime in mouse plasma was 4-2048 ng/mL with the lower limit of quantitation level (LLOQ) of 4 ng/mL. The inter- and intra-assay precision and accuracy of the method were below 9.05% and ranged from 95.6 to 113%, respectively, determined by quality control (QC) samples at five concentration levels including LLOQ. After [micro]Elution SPE, 71.1% of cefepime was recovered. The application of the validated assay for the determination of cefepime in mouse pharmacokinetics (PK) samples after intravenous (IV) and subcutaneous (SC) doses was demonstrated.</description><issn>1570-0232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNjs9OwzAMxnMAifHnEZB849QuXdnanachDnAaBySEpqx1G09JHJp0Y4_JGxFNPACSJVv29_38CXFfyLyQxWK6z_eNHtju8plMO1nm8nF-ISbFvJKZnJWzK3Edwl7KopJVORE_bxrB8QENBHSBIh0QlGtBU68hJtTYaz9GaDHiYMmpSOyAO2iwQ08WgRxYHgOCNypYBbsTbFbv2ctq-rpJBRaj5hY6NoaP5Ppk7jJDDuHDUjPw59qMZ-hykR3RpCBsqM28VomJ33FQzfkcGcLoPQ8RwilETOaUNNKOOKbRD9wPyoZbcdkpE_Dur9-Ih6f12-o5S4KvEUPcWgpN-qMcptjberko6rquqvL_yl_jYXng</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Bu, Wei</creator><creator>Sexton, Holly</creator><creator>Fan, Xiaoqing</creator><creator>Torres, Patricia</creator><creator>Houston, Paul</creator><creator>Heyman, Irwin</creator><creator>Liu, Liang</creator><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100601</creationdate><title>The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs</title><author>Bu, Wei ; Sexton, Holly ; Fan, Xiaoqing ; Torres, Patricia ; Houston, Paul ; Heyman, Irwin ; Liu, Liang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_8961888773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bu, Wei</creatorcontrib><creatorcontrib>Sexton, Holly</creatorcontrib><creatorcontrib>Fan, Xiaoqing</creatorcontrib><creatorcontrib>Torres, Patricia</creatorcontrib><creatorcontrib>Houston, Paul</creatorcontrib><creatorcontrib>Heyman, Irwin</creatorcontrib><creatorcontrib>Liu, Liang</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bu, Wei</au><au>Sexton, Holly</au><au>Fan, Xiaoqing</au><au>Torres, Patricia</au><au>Houston, Paul</au><au>Heyman, Irwin</au><au>Liu, Liang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><date>2010-06-01</date><risdate>2010</risdate><volume>878</volume><issue>19</issue><spage>1623</spage><epage>1628</epage><pages>1623-1628</pages><issn>1570-0232</issn><abstract>A sensitive and high throughput off-line [micro]Elution 96-well solid-phase extraction (SPE) followed by strong cation exchange (SCX) liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for determination of cefepime has been developed and validated in mouse plasma. Using the chemical analog, ceftazidime as an internal standard (IS), the linear range of the method for the determination of cefepime in mouse plasma was 4-2048 ng/mL with the lower limit of quantitation level (LLOQ) of 4 ng/mL. The inter- and intra-assay precision and accuracy of the method were below 9.05% and ranged from 95.6 to 113%, respectively, determined by quality control (QC) samples at five concentration levels including LLOQ. After [micro]Elution SPE, 71.1% of cefepime was recovered. The application of the validated assay for the determination of cefepime in mouse pharmacokinetics (PK) samples after intravenous (IV) and subcutaneous (SC) doses was demonstrated.</abstract><doi>10.1016/j.jchromb.2010.03.045</doi></addata></record>
fulltext fulltext
identifier ISSN: 1570-0232
ispartof Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-06, Vol.878 (19), p.1623-1628
issn 1570-0232
language eng
recordid cdi_proquest_miscellaneous_896188877
source Access via ScienceDirect (Elsevier)
title The novel sensitive and high throughput determination of cefepime in mouse plasma by SCX-LC/MS/MS method following off-line [micro]Elution 96-well solid-phase extraction to support systemic antibiotic programs
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T22%3A55%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20novel%20sensitive%20and%20high%20throughput%20determination%20of%20cefepime%20in%20mouse%20plasma%20by%20SCX-LC/MS/MS%20method%20following%20off-line%20%5Bmicro%5DElution%2096-well%20solid-phase%20extraction%20to%20support%20systemic%20antibiotic%20programs&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Bu,%20Wei&rft.date=2010-06-01&rft.volume=878&rft.issue=19&rft.spage=1623&rft.epage=1628&rft.pages=1623-1628&rft.issn=1570-0232&rft_id=info:doi/10.1016/j.jchromb.2010.03.045&rft_dat=%3Cproquest%3E896188877%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=896188877&rft_id=info:pmid/&rfr_iscdi=true