Enhanced Differentiation of Embryonic and Neural Stem Cells to Neuronal Fates on Laminin Peptides Doped Polypyrrole
PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroec...
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| Veröffentlicht in: | Macromolecular bioscience 2010-12, Vol.10 (12), p.1456-1464 |
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| creator | Zhang, Ling Stauffer, William R. Jane, Esther P. Sammak, Paul J. Cui, Xinyan Tracy |
| description | PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroectoderm formation from hESCs. After 14 d of culture, surfaces containing p20 showed the highest percentage of neuronal differentiation from hESC, while the PPy/p31 surface showed better cell attachment and spreading. In rNSCs cultures, a higher percentage of neurons were found on the PPy/p20 surface than other surfaces at 7 and 14 d. For differentiated neurons, p20 promoted both the primary and total neurite outgrowth. Longer primary neurites were found on p20‐containing surfaces and a longer total neurite length was found on PPy/p20 surface. These results demonstrated that, by doping PPy with different bioactive peptides, differentiation of stem cells seeded at different stages of development is affected.
hESCs and rNSCs are cultured on four kinds of PPy surfaces doped with laminin peptide p20 and p31. PPy/p20 promoted neuroectoderm formation and neuronal differentiation of hESCs, while PPy/p31 promoted adhesion and spreading of hESCs. PPy/p20 surfaces enhance the neuronal differentiation of rNSC by increasing not only the percentage of neurons but also the neurite length. Conductive polymer doped with peptide holds promise in manipulating neuronal differentiation of stem cells. |
| doi_str_mv | 10.1002/mabi.201000176 |
| format | Article |
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hESCs and rNSCs are cultured on four kinds of PPy surfaces doped with laminin peptide p20 and p31. PPy/p20 promoted neuroectoderm formation and neuronal differentiation of hESCs, while PPy/p31 promoted adhesion and spreading of hESCs. PPy/p20 surfaces enhance the neuronal differentiation of rNSC by increasing not only the percentage of neurons but also the neurite length. Conductive polymer doped with peptide holds promise in manipulating neuronal differentiation of stem cells.</description><identifier>ISSN: 1616-5187</identifier><identifier>ISSN: 1616-5195</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201000176</identifier><identifier>PMID: 20954199</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Amino Acids - analysis ; Applied sciences ; Axonogenesis ; Biological and medical sciences ; Biological properties ; Cell adhesion ; Cell culture ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell migration ; conducting polymers ; Developmental stages ; Differentiation ; Electrochemistry ; Embryo cells ; Embryonic Stem Cells - cytology ; Embryos ; Exact sciences and technology ; Humans ; Laminin ; Laminin - chemistry ; Laminin - pharmacology ; Macromolecules ; Medical sciences ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Neural stem cells ; Neural Stem Cells - cytology ; Neuroectoderm ; Neurons ; Neurons - cytology ; Organic polymers ; peptides ; Physicochemistry of polymers ; Polymerization ; Polymers - chemistry ; Polymers - pharmacology ; polypyrroles ; Properties and characterization ; Pyrroles - chemistry ; Pyrroles - pharmacology ; Stem cells ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; thin films ; Time Factors ; tissue engineering ; Tissue Engineering - methods</subject><ispartof>Macromolecular bioscience, 2010-12, Vol.10 (12), p.1456-1464</ispartof><rights>Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5836-e0064b3dc2b7c3485f7c7ceea6a07c1a3edd4b4777ecb5007240daa71afdc1a63</citedby><cites>FETCH-LOGICAL-c5836-e0064b3dc2b7c3485f7c7ceea6a07c1a3edd4b4777ecb5007240daa71afdc1a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201000176$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201000176$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23620256$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20954199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Stauffer, William R.</creatorcontrib><creatorcontrib>Jane, Esther P.</creatorcontrib><creatorcontrib>Sammak, Paul J.</creatorcontrib><creatorcontrib>Cui, Xinyan Tracy</creatorcontrib><title>Enhanced Differentiation of Embryonic and Neural Stem Cells to Neuronal Fates on Laminin Peptides Doped Polypyrrole</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroectoderm formation from hESCs. After 14 d of culture, surfaces containing p20 showed the highest percentage of neuronal differentiation from hESC, while the PPy/p31 surface showed better cell attachment and spreading. In rNSCs cultures, a higher percentage of neurons were found on the PPy/p20 surface than other surfaces at 7 and 14 d. For differentiated neurons, p20 promoted both the primary and total neurite outgrowth. Longer primary neurites were found on p20‐containing surfaces and a longer total neurite length was found on PPy/p20 surface. These results demonstrated that, by doping PPy with different bioactive peptides, differentiation of stem cells seeded at different stages of development is affected.
hESCs and rNSCs are cultured on four kinds of PPy surfaces doped with laminin peptide p20 and p31. PPy/p20 promoted neuroectoderm formation and neuronal differentiation of hESCs, while PPy/p31 promoted adhesion and spreading of hESCs. PPy/p20 surfaces enhance the neuronal differentiation of rNSC by increasing not only the percentage of neurons but also the neurite length. Conductive polymer doped with peptide holds promise in manipulating neuronal differentiation of stem cells.</description><subject>Amino Acids - analysis</subject><subject>Applied sciences</subject><subject>Axonogenesis</subject><subject>Biological and medical sciences</subject><subject>Biological properties</subject><subject>Cell adhesion</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell migration</subject><subject>conducting polymers</subject><subject>Developmental stages</subject><subject>Differentiation</subject><subject>Electrochemistry</subject><subject>Embryo cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryos</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Laminin</subject><subject>Laminin - chemistry</subject><subject>Laminin - pharmacology</subject><subject>Macromolecules</subject><subject>Medical sciences</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Electron, Scanning</subject><subject>Neural stem cells</subject><subject>Neural Stem Cells - cytology</subject><subject>Neuroectoderm</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Organic polymers</subject><subject>peptides</subject><subject>Physicochemistry of polymers</subject><subject>Polymerization</subject><subject>Polymers - chemistry</subject><subject>Polymers - pharmacology</subject><subject>polypyrroles</subject><subject>Properties and characterization</subject><subject>Pyrroles - chemistry</subject><subject>Pyrroles - pharmacology</subject><subject>Stem cells</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>thin films</subject><subject>Time Factors</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1616-5187</issn><issn>1616-5195</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2P0zAQxS0EYpfClSPyBcElxR-JnRx32-6HVJZFgEBcLMeZCENiBzsV5L_HoaVwYk8ezfzem7EeQk8pWVJC2Kte13bJSKoJleIeOqWCiqygVXH_WJfyBD2K8euMlBV7iE4YqYqcVtUpihv3RTsDDV7btoUAbrR6tN5h3-JNX4fJO2uwdg2-gV3QHX43Qo9X0HURj_5307vUvtAjRJx0W91bZx2-hWG0Teqt_ZDsb303DVMIvoPH6EGruwhPDu8CfbjYvF9dZds3l9ers21mipKLDAgRec0bw2ppeF4WrTTSAGihiTRUc2iavM6llGDqghDJctJoLalumzQWfIFe7H2H4L_vII6qt9Gky7UDv4uqrAQtOaP53SQtKsEEZ4l8-V-SEsYKwWUxo8s9aoKPMUCrhmB7HaYEqTk8NYenjuElwbOD967uoTnif9JKwPMDoKPRXRtScjb-5bhgZN6-QNWe-2E7mO5Yq16fnV__e0S219o4ws-jVodvSsj0L_Xx5lKVb3PyaV1dqc_8F43pwoc</recordid><startdate>20101208</startdate><enddate>20101208</enddate><creator>Zhang, Ling</creator><creator>Stauffer, William R.</creator><creator>Jane, Esther P.</creator><creator>Sammak, Paul J.</creator><creator>Cui, Xinyan Tracy</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20101208</creationdate><title>Enhanced Differentiation of Embryonic and Neural Stem Cells to Neuronal Fates on Laminin Peptides Doped Polypyrrole</title><author>Zhang, Ling ; Stauffer, William R. ; Jane, Esther P. ; Sammak, Paul J. ; Cui, Xinyan Tracy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5836-e0064b3dc2b7c3485f7c7ceea6a07c1a3edd4b4777ecb5007240daa71afdc1a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acids - analysis</topic><topic>Applied sciences</topic><topic>Axonogenesis</topic><topic>Biological and medical sciences</topic><topic>Biological properties</topic><topic>Cell adhesion</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell migration</topic><topic>conducting polymers</topic><topic>Developmental stages</topic><topic>Differentiation</topic><topic>Electrochemistry</topic><topic>Embryo cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryos</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Laminin</topic><topic>Laminin - chemistry</topic><topic>Laminin - pharmacology</topic><topic>Macromolecules</topic><topic>Medical sciences</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Electron, Scanning</topic><topic>Neural stem cells</topic><topic>Neural Stem Cells - cytology</topic><topic>Neuroectoderm</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Organic polymers</topic><topic>peptides</topic><topic>Physicochemistry of polymers</topic><topic>Polymerization</topic><topic>Polymers - chemistry</topic><topic>Polymers - pharmacology</topic><topic>polypyrroles</topic><topic>Properties and characterization</topic><topic>Pyrroles - chemistry</topic><topic>Pyrroles - pharmacology</topic><topic>Stem cells</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>thin films</topic><topic>Time Factors</topic><topic>tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Stauffer, William R.</creatorcontrib><creatorcontrib>Jane, Esther P.</creatorcontrib><creatorcontrib>Sammak, Paul J.</creatorcontrib><creatorcontrib>Cui, Xinyan Tracy</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Ling</au><au>Stauffer, William R.</au><au>Jane, Esther P.</au><au>Sammak, Paul J.</au><au>Cui, Xinyan Tracy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Differentiation of Embryonic and Neural Stem Cells to Neuronal Fates on Laminin Peptides Doped Polypyrrole</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2010-12-08</date><risdate>2010</risdate><volume>10</volume><issue>12</issue><spage>1456</spage><epage>1464</epage><pages>1456-1464</pages><issn>1616-5187</issn><issn>1616-5195</issn><eissn>1616-5195</eissn><abstract>PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroectoderm formation from hESCs. After 14 d of culture, surfaces containing p20 showed the highest percentage of neuronal differentiation from hESC, while the PPy/p31 surface showed better cell attachment and spreading. In rNSCs cultures, a higher percentage of neurons were found on the PPy/p20 surface than other surfaces at 7 and 14 d. For differentiated neurons, p20 promoted both the primary and total neurite outgrowth. Longer primary neurites were found on p20‐containing surfaces and a longer total neurite length was found on PPy/p20 surface. These results demonstrated that, by doping PPy with different bioactive peptides, differentiation of stem cells seeded at different stages of development is affected.
hESCs and rNSCs are cultured on four kinds of PPy surfaces doped with laminin peptide p20 and p31. PPy/p20 promoted neuroectoderm formation and neuronal differentiation of hESCs, while PPy/p31 promoted adhesion and spreading of hESCs. PPy/p20 surfaces enhance the neuronal differentiation of rNSC by increasing not only the percentage of neurons but also the neurite length. Conductive polymer doped with peptide holds promise in manipulating neuronal differentiation of stem cells.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>20954199</pmid><doi>10.1002/mabi.201000176</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acids - analysis Applied sciences Axonogenesis Biological and medical sciences Biological properties Cell adhesion Cell culture Cell Differentiation - drug effects Cell Differentiation - physiology Cell migration conducting polymers Developmental stages Differentiation Electrochemistry Embryo cells Embryonic Stem Cells - cytology Embryos Exact sciences and technology Humans Laminin Laminin - chemistry Laminin - pharmacology Macromolecules Medical sciences Microscopy, Atomic Force Microscopy, Electron, Scanning Neural stem cells Neural Stem Cells - cytology Neuroectoderm Neurons Neurons - cytology Organic polymers peptides Physicochemistry of polymers Polymerization Polymers - chemistry Polymers - pharmacology polypyrroles Properties and characterization Pyrroles - chemistry Pyrroles - pharmacology Stem cells Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments thin films Time Factors tissue engineering Tissue Engineering - methods |
| title | Enhanced Differentiation of Embryonic and Neural Stem Cells to Neuronal Fates on Laminin Peptides Doped Polypyrrole |
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