Rapid Pyrophosphate Release from Transcriptional Elongation Complexes Appears to be Coupled to a Nucleotide-Induced Conformational Change in E. coli Core Polymerase

In the nucleotide addition cycle, pyrophosphate is generated upon incorporation of each nucleotide. Rapid release of pyrophosphate is essential for facile transcription elongation. Stopped-flow kinetic studies involving alterations in the intrinsic protein fluorescence of the core polymerase upon th...

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