Method for Simultaneous Imaging of Endogenous Low Molecular Weight Metabolites in Mouse Brain Using TiO2 Nanoparticles in Nanoparticle-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry
We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80–500 Da) using TiO2 nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-PALDI-IMS) without any washing and separation step prior to MS analy...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2011-10, Vol.83 (19), p.7283-7289 |
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| creator | Shrivas, Kamlesh Hayasaka, Takahiro Sugiura, Yuki Setou, Mitsutoshi |
| description | We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80–500 Da) using TiO2 nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-PALDI-IMS) without any washing and separation step prior to MS analysis. The identification of metabolites using TiO2 NPs was compared with a conventional organic matrix 2,5-dihydroxybenzoic acid (DHB) where signals of 179 molecules were specific to TiO2 NPs, 4 were specific to DHB, and 21 were common to both TiO2 NPs and DHB. The use of TiO2 NPs enabled the detection of a higher number of LMWM as compared to DHB and gold NPs as a matrix. This approach is a simple, inexpensive, washing, and separation free for imaging and identification of LMWM in mouse brain. We believe that the biochemical information from distinct regions of the brain using a Nano-PALDI-IMS will be helpful in elucidating the imbalances linked with diseases in biomedical samples. |
| doi_str_mv | 10.1021/ac201602s |
| format | Article |
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The identification of metabolites using TiO2 NPs was compared with a conventional organic matrix 2,5-dihydroxybenzoic acid (DHB) where signals of 179 molecules were specific to TiO2 NPs, 4 were specific to DHB, and 21 were common to both TiO2 NPs and DHB. The use of TiO2 NPs enabled the detection of a higher number of LMWM as compared to DHB and gold NPs as a matrix. This approach is a simple, inexpensive, washing, and separation free for imaging and identification of LMWM in mouse brain. 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We believe that the biochemical information from distinct regions of the brain using a Nano-PALDI-IMS will be helpful in elucidating the imbalances linked with diseases in biomedical samples.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Histidine - analysis</subject><subject>Histidine - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Nanoparticles - chemistry</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Surface Properties</subject><subject>Titanium - chemistry</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc1u1DAUhS0EokNhwQsgbxCrUNuZ_HhZSoGRZuiirVhGN_HN1JVjB19HqLwf70WimUJX9-qcT0f3h7G3UnyUQskz6JSQpVD0jK1koURW1rV6zlZCiDxTlRAn7BXRvRBSztxLdqJkrde6XK_Ynx2mu2B4HyK_tsPkEngME_HNAHvr9zz0_NKbsEe_qNvwi--Cw25yEPkPtPu7xOcIaIOzCYlbP_sTIf8UYe5vacm4sVeKfwcfRojJdu7APRWycyJLCQ3fAmHkn5FCHJMN_mwTvP0NS5s9zrQDIn49YpdiGDDFh9fsRQ-O8M2xnrLbL5c3F9-y7dXXzcX5NoM8L1OmRdlXVaVUD8pAYeoyxzovlQYtdSewVm1b5KYowUArjNZyLbCtQPb94lT5KftwyB1j-DkhpWaw1KFzh6M1tS7qoioKOZPvjuTUDmiaMdoB4kPzePkZeH8EgDpwfQTfWfrPrYtSV-oJBx0192GKfl6wkaJZPt_8-3z-F_4docE</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Shrivas, Kamlesh</creator><creator>Hayasaka, Takahiro</creator><creator>Sugiura, Yuki</creator><creator>Setou, Mitsutoshi</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Method for Simultaneous Imaging of Endogenous Low Molecular Weight Metabolites in Mouse Brain Using TiO2 Nanoparticles in Nanoparticle-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry</title><author>Shrivas, Kamlesh ; Hayasaka, Takahiro ; Sugiura, Yuki ; Setou, Mitsutoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-906f77722fa2da5d863e83629a919c0e82bb53d56adab0d99140eb7a1ff2bb573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Histidine - analysis</topic><topic>Histidine - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Nanoparticles - chemistry</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Surface Properties</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shrivas, Kamlesh</creatorcontrib><creatorcontrib>Hayasaka, Takahiro</creatorcontrib><creatorcontrib>Sugiura, Yuki</creatorcontrib><creatorcontrib>Setou, Mitsutoshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shrivas, Kamlesh</au><au>Hayasaka, Takahiro</au><au>Sugiura, Yuki</au><au>Setou, Mitsutoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Method for Simultaneous Imaging of Endogenous Low Molecular Weight Metabolites in Mouse Brain Using TiO2 Nanoparticles in Nanoparticle-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>83</volume><issue>19</issue><spage>7283</spage><epage>7289</epage><pages>7283-7289</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80–500 Da) using TiO2 nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-PALDI-IMS) without any washing and separation step prior to MS analysis. The identification of metabolites using TiO2 NPs was compared with a conventional organic matrix 2,5-dihydroxybenzoic acid (DHB) where signals of 179 molecules were specific to TiO2 NPs, 4 were specific to DHB, and 21 were common to both TiO2 NPs and DHB. The use of TiO2 NPs enabled the detection of a higher number of LMWM as compared to DHB and gold NPs as a matrix. This approach is a simple, inexpensive, washing, and separation free for imaging and identification of LMWM in mouse brain. We believe that the biochemical information from distinct regions of the brain using a Nano-PALDI-IMS will be helpful in elucidating the imbalances linked with diseases in biomedical samples.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21894964</pmid><doi>10.1021/ac201602s</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2011-10, Vol.83 (19), p.7283-7289 |
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| subjects | Analytical chemistry Animals Brain - metabolism Chemistry Exact sciences and technology Histidine - analysis Histidine - metabolism Male Mice Molecular Weight Nanoparticles - chemistry Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Properties Titanium - chemistry |
| title | Method for Simultaneous Imaging of Endogenous Low Molecular Weight Metabolites in Mouse Brain Using TiO2 Nanoparticles in Nanoparticle-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry |
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