Highly Sensitive Pyrosequencing System with Polymer-Supported Enzymes for High-Throughput DNA Analysis

A highly sensitive massively parallel pyrosequencing system employing a gel matrix to immobilize enzymes at high density in microreaction chambers is demonstrated. Reducing the size of microreaction chambers in a DNA analyzer is important to achieve a high throughput utilizing a commercially availab...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-10, Vol.83 (19), p.7560-7565
Hauptverfasser:	Shirai, Masataka, Goto, Mari, Suzuki, Shigeya, Kono, Kenji, Kajiyama, Tomoharu, Kambara, Hideki
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Analyzers Chemistry Deoxyribonucleic acid DNA DNA - analysis DNA - genetics Enzymes Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Exact sciences and technology High-Throughput Nucleotide Sequencing - methods Luciferases - chemistry Luciferases - metabolism Luminescence Polymers - chemistry Spectrometric and optical methods Sulfate Adenylyltransferase - chemistry Sulfate Adenylyltransferase - metabolism
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