Kinetics of DNA Refolding from Longitudinal Exchange NMR Spectroscopy

Kinetics of DNA Refolding from Longitudinal Exchange NMR Spectroscopy

Switching of DNA: We address the conformational heterogeneity of DNA on the secondary structure level. While it has been demonstrated that RNA is able to modulate function by changing its folding state, we show for the first time that DNA too can undergo transitions between energetically similar fol...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2011-09, Vol.12 (13), p.2007-2010
Hauptverfasser: Spitzer, Romana, Kloiber, Karin, Tollinger, Martin, Kreutz, Christoph
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
bistable DNA
DNA
DNA - chemistry
Humans
Kinetics
longitudinal exchange spectroscopy
Molecular Sequence Data
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
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Zusammenfassung:Switching of DNA: We address the conformational heterogeneity of DNA on the secondary structure level. While it has been demonstrated that RNA is able to modulate function by changing its folding state, we show for the first time that DNA too can undergo transitions between energetically similar folding states on a time scale that is relevant for biological processes (see figure).
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201100318