Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL)
The objective of this study was to investigate expression of drug resistance associated genes in CD34+ and CD34- leukemic subpopulations in childhood acute lymphoblastic leukemia (ALL). ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations...
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| Veröffentlicht in: | Experimental oncology 2011-09, Vol.33 (3), p.145-149 |
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| container_title | Experimental oncology |
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| creator | Shman, T V Fedasenka, U U Savitski, V P Kustanovich, A M Aleinikova, O V |
| description | The objective of this study was to investigate expression of drug resistance associated genes in CD34+ and CD34- leukemic subpopulations in childhood acute lymphoblastic leukemia (ALL).
ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations and mRNA levels of MDR1, LRP, BCRP and BCL-2 genes were compared.
BCL-2 gene expression levels did not differ significantly between CD34+ vs CD34- subpopulations in most analyzed ALL cases. Oppositely, MDR1 gene had >two-fold differences in expression levels between subpopulations in the majority of ALL cases. In T-lineage ALL CD34- fractions had increased level of BCRP and LRP genes in comparison with CD34+ ones whereas in most of B-lineage ALL expression of these genes did not differ.
It was not found the unique pattern of resistance related genes expression in CD34+ vs CD34- subpopulations. However, in majority of studied pediatric ALL cases with CD34 heterogeneous expression one of subpopulations (positive or negative) could have an advantage for survival through elevated expression of drug resistance related genes. |
| format | Article |
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ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations and mRNA levels of MDR1, LRP, BCRP and BCL-2 genes were compared.
BCL-2 gene expression levels did not differ significantly between CD34+ vs CD34- subpopulations in most analyzed ALL cases. Oppositely, MDR1 gene had >two-fold differences in expression levels between subpopulations in the majority of ALL cases. In T-lineage ALL CD34- fractions had increased level of BCRP and LRP genes in comparison with CD34+ ones whereas in most of B-lineage ALL expression of these genes did not differ.
It was not found the unique pattern of resistance related genes expression in CD34+ vs CD34- subpopulations. However, in majority of studied pediatric ALL cases with CD34 heterogeneous expression one of subpopulations (positive or negative) could have an advantage for survival through elevated expression of drug resistance related genes.</description><identifier>ISSN: 1812-9269</identifier><identifier>PMID: 21956467</identifier><language>eng</language><publisher>Ukraine</publisher><subject>Adolescent ; Antigens, CD34 - analysis ; ATP Binding Cassette Transporter, Sub-Family B ; ATP Binding Cassette Transporter, Sub-Family G, Member 2 ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ; Cell Line, Tumor ; Child ; Child, Preschool ; Drug Resistance, Neoplasm - genetics ; Female ; Humans ; Infant ; Male ; Neoplasm Proteins - genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism ; Proto-Oncogene Proteins c-bcl-2 - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vault Ribonucleoprotein Particles - genetics</subject><ispartof>Experimental oncology, 2011-09, Vol.33 (3), p.145-149</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21956467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shman, T V</creatorcontrib><creatorcontrib>Fedasenka, U U</creatorcontrib><creatorcontrib>Savitski, V P</creatorcontrib><creatorcontrib>Kustanovich, A M</creatorcontrib><creatorcontrib>Aleinikova, O V</creatorcontrib><title>Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL)</title><title>Experimental oncology</title><addtitle>Exp Oncol</addtitle><description>The objective of this study was to investigate expression of drug resistance associated genes in CD34+ and CD34- leukemic subpopulations in childhood acute lymphoblastic leukemia (ALL).
ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations and mRNA levels of MDR1, LRP, BCRP and BCL-2 genes were compared.
BCL-2 gene expression levels did not differ significantly between CD34+ vs CD34- subpopulations in most analyzed ALL cases. Oppositely, MDR1 gene had >two-fold differences in expression levels between subpopulations in the majority of ALL cases. In T-lineage ALL CD34- fractions had increased level of BCRP and LRP genes in comparison with CD34+ ones whereas in most of B-lineage ALL expression of these genes did not differ.
It was not found the unique pattern of resistance related genes expression in CD34+ vs CD34- subpopulations. However, in majority of studied pediatric ALL cases with CD34 heterogeneous expression one of subpopulations (positive or negative) could have an advantage for survival through elevated expression of drug resistance related genes.</description><subject>Adolescent</subject><subject>Antigens, CD34 - analysis</subject><subject>ATP Binding Cassette Transporter, Sub-Family B</subject><subject>ATP Binding Cassette Transporter, Sub-Family G, Member 2</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</subject><subject>Cell Line, Tumor</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Drug Resistance, Neoplasm - genetics</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Neoplasm Proteins - genetics</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vault Ribonucleoprotein Particles - genetics</subject><issn>1812-9269</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kLtOwzAYhTOAaCm8AvIGDJFiJ3HssWq5SZFYYI58-UMMTmxiW9CH4J0pUKYznE-fdM5RtsQMk5wTyhfZaQivRUFrTquTbEEwr2lFm2X2tZ3TC5ohmBDFpACJEJwyIoJGfnYe5mggINcjaUWIAYUkvfPJimjcFNCHiQPSpu9hhimizbasEHz6vTDse2QmpAZj9eCcRkKlCMjuRj-4X5tRyEJ6g9EIdLVu2-uz7LgXNsD5IVfZ8-3N0-Y-bx_vHjbrNveY1DFXTdPgEtcEayioZLjQRFDCeK2YpKIvmQBdyEJzIjkAF6Kp-l7KuuGNUpKVq-zyz7uf-J4gxG40QYG1YgKXQrc3sbqk7Ie8OJBJjqA7P5tRzLvu_8HyG4LDb5Q</recordid><startdate>201109</startdate><enddate>201109</enddate><creator>Shman, T V</creator><creator>Fedasenka, U U</creator><creator>Savitski, V P</creator><creator>Kustanovich, A M</creator><creator>Aleinikova, O V</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201109</creationdate><title>Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL)</title><author>Shman, T V ; Fedasenka, U U ; Savitski, V P ; Kustanovich, A M ; Aleinikova, O V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p125t-c777131521de06b810d2a62895c8b6af38aed0b0d92b9ee9aa74ffbb5797ccb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Antigens, CD34 - analysis</topic><topic>ATP Binding Cassette Transporter, Sub-Family B</topic><topic>ATP Binding Cassette Transporter, Sub-Family G, Member 2</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</topic><topic>Cell Line, Tumor</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Drug Resistance, Neoplasm - genetics</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Neoplasm Proteins - genetics</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vault Ribonucleoprotein Particles - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shman, T V</creatorcontrib><creatorcontrib>Fedasenka, U U</creatorcontrib><creatorcontrib>Savitski, V P</creatorcontrib><creatorcontrib>Kustanovich, A M</creatorcontrib><creatorcontrib>Aleinikova, O V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shman, T V</au><au>Fedasenka, U U</au><au>Savitski, V P</au><au>Kustanovich, A M</au><au>Aleinikova, O V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL)</atitle><jtitle>Experimental oncology</jtitle><addtitle>Exp Oncol</addtitle><date>2011-09</date><risdate>2011</risdate><volume>33</volume><issue>3</issue><spage>145</spage><epage>149</epage><pages>145-149</pages><issn>1812-9269</issn><abstract>The objective of this study was to investigate expression of drug resistance associated genes in CD34+ and CD34- leukemic subpopulations in childhood acute lymphoblastic leukemia (ALL).
ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations and mRNA levels of MDR1, LRP, BCRP and BCL-2 genes were compared.
BCL-2 gene expression levels did not differ significantly between CD34+ vs CD34- subpopulations in most analyzed ALL cases. Oppositely, MDR1 gene had >two-fold differences in expression levels between subpopulations in the majority of ALL cases. In T-lineage ALL CD34- fractions had increased level of BCRP and LRP genes in comparison with CD34+ ones whereas in most of B-lineage ALL expression of these genes did not differ.
It was not found the unique pattern of resistance related genes expression in CD34+ vs CD34- subpopulations. However, in majority of studied pediatric ALL cases with CD34 heterogeneous expression one of subpopulations (positive or negative) could have an advantage for survival through elevated expression of drug resistance related genes.</abstract><cop>Ukraine</cop><pmid>21956467</pmid><tpages>5</tpages></addata></record> |
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| language | eng |
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| subjects | Adolescent Antigens, CD34 - analysis ATP Binding Cassette Transporter, Sub-Family B ATP Binding Cassette Transporter, Sub-Family G, Member 2 ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - genetics Cell Line, Tumor Child Child, Preschool Drug Resistance, Neoplasm - genetics Female Humans Infant Male Neoplasm Proteins - genetics Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism Proto-Oncogene Proteins c-bcl-2 - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Vault Ribonucleoprotein Particles - genetics |
| title | Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL) |
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