Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity
Objective Free fetal DNA (ffDNA) in the maternal plasma appears to originate mainly from the trophoblast. We tested the hypothesis that ffDNA concentration is increased in multiple pregnancies where trophoblastic mass has been shown to be increased. Methods Quantitative real‐time PCR was used to mea...
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| Veröffentlicht in: | Prenatal diagnosis 2011-10, Vol.31 (10), p.967-972 |
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| creator | Attilakos, George Maddocks, Deborah G. Davies, Teresa Hunt, Linda P. Avent, Neil D. Soothill, Peter W. Grant, Simon R. |
| description | Objective
Free fetal DNA (ffDNA) in the maternal plasma appears to originate mainly from the trophoblast. We tested the hypothesis that ffDNA concentration is increased in multiple pregnancies where trophoblastic mass has been shown to be increased.
Methods
Quantitative real‐time PCR was used to measure the plasma concentration of DYS14 in singleton and twin pregnancies with one or two male fetuses. Royston and Wright's regression method was used to relate ffDNA to gestational age in singleton controls; z‐scores were calculated for the multiple pregnancy subgroups.
Results
Fifty‐five singleton and 65 twin pregnancies (36 with one and 29 with two male fetuses) were analysed. There was significantly higher ffDNA concentration in twin pregnancies with two male fetuses compared with pregnancies with one male fetus. In cases with two male fetuses, there was no statistically significant difference between monochorionic and dichorionic pregnancies.
Conclusions
There is higher ffDNA concentration in multiple pregnancies, and this must be taken into account for future quantitative ffDNA applications. Copyright © 2011 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pd.2814 |
| format | Article |
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Free fetal DNA (ffDNA) in the maternal plasma appears to originate mainly from the trophoblast. We tested the hypothesis that ffDNA concentration is increased in multiple pregnancies where trophoblastic mass has been shown to be increased.
Methods
Quantitative real‐time PCR was used to measure the plasma concentration of DYS14 in singleton and twin pregnancies with one or two male fetuses. Royston and Wright's regression method was used to relate ffDNA to gestational age in singleton controls; z‐scores were calculated for the multiple pregnancy subgroups.
Results
Fifty‐five singleton and 65 twin pregnancies (36 with one and 29 with two male fetuses) were analysed. There was significantly higher ffDNA concentration in twin pregnancies with two male fetuses compared with pregnancies with one male fetus. In cases with two male fetuses, there was no statistically significant difference between monochorionic and dichorionic pregnancies.
Conclusions
There is higher ffDNA concentration in multiple pregnancies, and this must be taken into account for future quantitative ffDNA applications. Copyright © 2011 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0197-3851</identifier><identifier>ISSN: 1097-0223</identifier><identifier>EISSN: 1097-0223</identifier><identifier>DOI: 10.1002/pd.2814</identifier><identifier>PMID: 21769896</identifier><identifier>CODEN: PRDIDM</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Biomarkers - blood ; Cell Cycle Proteins - blood ; Cell Cycle Proteins - genetics ; Chorion - anatomy & histology ; Chorion - metabolism ; Chorionic Gonadotropin, beta Subunit, Human - blood ; Chromosomes, Human, Y - genetics ; Delivery. Postpartum. Lactation ; DNA - blood ; Female ; fetal DNA ; fetal nucleic acids ; Fetus - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Gynecology. Andrology. Obstetrics ; Humans ; Male ; Medical sciences ; Middle Aged ; Molecular and cellular biology ; multiple pregnancies ; Pregnancy ; Pregnancy, Twin - blood ; prenatal diagnosis ; Prenatal Diagnosis - methods ; Twins ; Young Adult</subject><ispartof>Prenatal diagnosis, 2011-10, Vol.31 (10), p.967-972</ispartof><rights>Copyright © 2011 John Wiley & Sons, Ltd.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4174-dff4edda289222d428c1d3c2907743dda8053eb9c1f03b3ec87250c982cbd88c3</citedby><cites>FETCH-LOGICAL-c4174-dff4edda289222d428c1d3c2907743dda8053eb9c1f03b3ec87250c982cbd88c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpd.2814$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpd.2814$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24536824$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21769896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Attilakos, George</creatorcontrib><creatorcontrib>Maddocks, Deborah G.</creatorcontrib><creatorcontrib>Davies, Teresa</creatorcontrib><creatorcontrib>Hunt, Linda P.</creatorcontrib><creatorcontrib>Avent, Neil D.</creatorcontrib><creatorcontrib>Soothill, Peter W.</creatorcontrib><creatorcontrib>Grant, Simon R.</creatorcontrib><title>Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity</title><title>Prenatal diagnosis</title><addtitle>Prenat. Diagn</addtitle><description>Objective
Free fetal DNA (ffDNA) in the maternal plasma appears to originate mainly from the trophoblast. We tested the hypothesis that ffDNA concentration is increased in multiple pregnancies where trophoblastic mass has been shown to be increased.
Methods
Quantitative real‐time PCR was used to measure the plasma concentration of DYS14 in singleton and twin pregnancies with one or two male fetuses. Royston and Wright's regression method was used to relate ffDNA to gestational age in singleton controls; z‐scores were calculated for the multiple pregnancy subgroups.
Results
Fifty‐five singleton and 65 twin pregnancies (36 with one and 29 with two male fetuses) were analysed. There was significantly higher ffDNA concentration in twin pregnancies with two male fetuses compared with pregnancies with one male fetus. In cases with two male fetuses, there was no statistically significant difference between monochorionic and dichorionic pregnancies.
Conclusions
There is higher ffDNA concentration in multiple pregnancies, and this must be taken into account for future quantitative ffDNA applications. Copyright © 2011 John Wiley & Sons, Ltd.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Cell Cycle Proteins - blood</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Chorion - anatomy & histology</subject><subject>Chorion - metabolism</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - blood</subject><subject>Chromosomes, Human, Y - genetics</subject><subject>Delivery. Postpartum. Lactation</subject><subject>DNA - blood</subject><subject>Female</subject><subject>fetal DNA</subject><subject>fetal nucleic acids</subject><subject>Fetus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular and cellular biology</subject><subject>multiple pregnancies</subject><subject>Pregnancy</subject><subject>Pregnancy, Twin - blood</subject><subject>prenatal diagnosis</subject><subject>Prenatal Diagnosis - methods</subject><subject>Twins</subject><subject>Young Adult</subject><issn>0197-3851</issn><issn>1097-0223</issn><issn>1097-0223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90EFvFCEUB3BiNHZbjd_AcDGamKnwYAY4Nq2tmqZWo_HggbDwcNHZmRFmUvfbO-uu7UlPEN6P_0v-hDzh7JgzBq-GcAyay3tkwZlRFQMQ98mC8fkudM0PyGEp32eowaiH5AC4aow2zYJ8_TC5bkwxeTemvqN9pDEj0oija-nZ1QlNHV1P7ZiGFumQ8VvnOp-wUNcFmrH9862s0kBv0riiftXn-SH5NG4ekQfRtQUf788j8vn89afTN9Xl-4u3pyeXlZdcySrEKDEEB9oAQJCgPQ_Cg2FKSTEPNKsFLo3nkYmlQK8V1MwbDX4ZtPbiiDzf5Q65_zlhGe06FY9t6zrsp2K1kZo30MAsX_xXcgZMSyaZuQv1uS8lY7RDTmuXNzOy287tEOy281k-3YdOyzWGW_e35Bk82wNXvGtj3jZY7pysRaNhG_Ry525Si5t_7bPXZ_u11U6nMuKvW-3yD9sooWr75erCMnGt3zUfhdXiN8R-pWk</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Attilakos, George</creator><creator>Maddocks, Deborah G.</creator><creator>Davies, Teresa</creator><creator>Hunt, Linda P.</creator><creator>Avent, Neil D.</creator><creator>Soothill, Peter W.</creator><creator>Grant, Simon R.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity</title><author>Attilakos, George ; Maddocks, Deborah G. ; Davies, Teresa ; Hunt, Linda P. ; Avent, Neil D. ; Soothill, Peter W. ; Grant, Simon R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4174-dff4edda289222d428c1d3c2907743dda8053eb9c1f03b3ec87250c982cbd88c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Cell Cycle Proteins - blood</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Chorion - anatomy & histology</topic><topic>Chorion - metabolism</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - blood</topic><topic>Chromosomes, Human, Y - genetics</topic><topic>Delivery. Postpartum. Lactation</topic><topic>DNA - blood</topic><topic>Female</topic><topic>fetal DNA</topic><topic>fetal nucleic acids</topic><topic>Fetus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular and cellular biology</topic><topic>multiple pregnancies</topic><topic>Pregnancy</topic><topic>Pregnancy, Twin - blood</topic><topic>prenatal diagnosis</topic><topic>Prenatal Diagnosis - methods</topic><topic>Twins</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Attilakos, George</creatorcontrib><creatorcontrib>Maddocks, Deborah G.</creatorcontrib><creatorcontrib>Davies, Teresa</creatorcontrib><creatorcontrib>Hunt, Linda P.</creatorcontrib><creatorcontrib>Avent, Neil D.</creatorcontrib><creatorcontrib>Soothill, Peter W.</creatorcontrib><creatorcontrib>Grant, Simon R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Prenatal diagnosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Attilakos, George</au><au>Maddocks, Deborah G.</au><au>Davies, Teresa</au><au>Hunt, Linda P.</au><au>Avent, Neil D.</au><au>Soothill, Peter W.</au><au>Grant, Simon R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity</atitle><jtitle>Prenatal diagnosis</jtitle><addtitle>Prenat. Diagn</addtitle><date>2011-10</date><risdate>2011</risdate><volume>31</volume><issue>10</issue><spage>967</spage><epage>972</epage><pages>967-972</pages><issn>0197-3851</issn><issn>1097-0223</issn><eissn>1097-0223</eissn><coden>PRDIDM</coden><abstract>Objective
Free fetal DNA (ffDNA) in the maternal plasma appears to originate mainly from the trophoblast. We tested the hypothesis that ffDNA concentration is increased in multiple pregnancies where trophoblastic mass has been shown to be increased.
Methods
Quantitative real‐time PCR was used to measure the plasma concentration of DYS14 in singleton and twin pregnancies with one or two male fetuses. Royston and Wright's regression method was used to relate ffDNA to gestational age in singleton controls; z‐scores were calculated for the multiple pregnancy subgroups.
Results
Fifty‐five singleton and 65 twin pregnancies (36 with one and 29 with two male fetuses) were analysed. There was significantly higher ffDNA concentration in twin pregnancies with two male fetuses compared with pregnancies with one male fetus. In cases with two male fetuses, there was no statistically significant difference between monochorionic and dichorionic pregnancies.
Conclusions
There is higher ffDNA concentration in multiple pregnancies, and this must be taken into account for future quantitative ffDNA applications. Copyright © 2011 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>21769896</pmid><doi>10.1002/pd.2814</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Adult Biological and medical sciences Biomarkers - blood Cell Cycle Proteins - blood Cell Cycle Proteins - genetics Chorion - anatomy & histology Chorion - metabolism Chorionic Gonadotropin, beta Subunit, Human - blood Chromosomes, Human, Y - genetics Delivery. Postpartum. Lactation DNA - blood Female fetal DNA fetal nucleic acids Fetus - metabolism Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Gynecology. Andrology. Obstetrics Humans Male Medical sciences Middle Aged Molecular and cellular biology multiple pregnancies Pregnancy Pregnancy, Twin - blood prenatal diagnosis Prenatal Diagnosis - methods Twins Young Adult |
| title | Quantification of free fetal DNA in multiple pregnancies and relationship with chorionicity |
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