Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation
The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Im...
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| Veröffentlicht in: | Radiation research 2011-10, Vol.176 (4), p.415-424 |
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| creator | Young, Erik F. Smilenov, Lubomir B. |
| description | The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported. |
| doi_str_mv | 10.1667/RR2665.1 |
| format | Article |
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Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.1667/RR2665.1</identifier><identifier>PMID: 21861763</identifier><language>eng</language><publisher>United States: The Radiation Research Society</publisher><subject>Cell Movement - radiation effects ; Coronary Vessels - cytology ; Cytoskeleton - radiation effects ; Electric Impedance ; Electrodes ; Endothelial cells ; Endothelium ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Endothelium, Vascular - radiation effects ; Humans ; Ionizing radiation ; Irradiation ; Kinetics ; Models, Biological ; Molecular Imaging ; Permeability - radiation effects ; Radiation damage ; Radiation dosage ; Radiotherapy ; REGULAR ARTICLES ; Space life sciences ; Statistical significance ; Time Factors</subject><ispartof>Radiation research, 2011-10, Vol.176 (4), p.415-424</ispartof><rights>Copyright © 2011 Radiation Research Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b369t-c598b1436faa8f0e62614da0f908d62372f067c90a0fb1891ba3098271d89fd93</citedby><cites>FETCH-LOGICAL-b369t-c598b1436faa8f0e62614da0f908d62372f067c90a0fb1891ba3098271d89fd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1667/RR2665.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41318208$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26976,27922,27923,52361,58015,58248</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21861763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Young, Erik F.</creatorcontrib><creatorcontrib>Smilenov, Lubomir B.</creatorcontrib><title>Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported.</description><subject>Cell Movement - radiation effects</subject><subject>Coronary Vessels - cytology</subject><subject>Cytoskeleton - radiation effects</subject><subject>Electric Impedance</subject><subject>Electrodes</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium, Vascular - radiation effects</subject><subject>Humans</subject><subject>Ionizing radiation</subject><subject>Irradiation</subject><subject>Kinetics</subject><subject>Models, Biological</subject><subject>Molecular Imaging</subject><subject>Permeability - radiation effects</subject><subject>Radiation damage</subject><subject>Radiation dosage</subject><subject>Radiotherapy</subject><subject>REGULAR ARTICLES</subject><subject>Space life sciences</subject><subject>Statistical significance</subject><subject>Time Factors</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFv1DAQhS1ERZeCxB8A-QRcUjxx4thHWC2wUhFoKefIicetq8RebAex_ID-7maV0ltPo5n36Y3eI-QVsHMQovmw25VC1OfwhKxAcVnUFauekhVjnBdNLZtT8jylGzbvINQzclqCFNAIviK323GPRvsei086oaE_p_gH3TAcTzRYehm1Tw59pj8wjqg7N7h8oOtr7a8wUefpOsTgdTzQjTchX-Pg9EC_BR8GfcCYqLYZI9383Yc0RaQ50G3w7p_zV3SnjdPZBf-CnFg9JHx5P8_Ir8-by_XX4uL7l-3640XRcaFy0ddKdlBxYbWWlqEoBVRGM6uYNKLkTWmZaHrF5lMHUkGnOVOybMBIZY3iZ-Td4ruP4feEKbejSz0e42KYUitVJaHmDczk-4XsY0gpom330Y1zzBZYeyy9XUpvj-ibe9OpG9E8gP9bnoHXC3CTcogPegUcZMnkrL9d9M6F4PHxT3dK25Pt</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Young, Erik F.</creator><creator>Smilenov, Lubomir B.</creator><general>The Radiation Research Society</general><general>Radiation Research Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation</title><author>Young, Erik F. ; Smilenov, Lubomir B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b369t-c598b1436faa8f0e62614da0f908d62372f067c90a0fb1891ba3098271d89fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Cell Movement - radiation effects</topic><topic>Coronary Vessels - cytology</topic><topic>Cytoskeleton - radiation effects</topic><topic>Electric Impedance</topic><topic>Electrodes</topic><topic>Endothelial cells</topic><topic>Endothelium</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium, Vascular - radiation effects</topic><topic>Humans</topic><topic>Ionizing radiation</topic><topic>Irradiation</topic><topic>Kinetics</topic><topic>Models, Biological</topic><topic>Molecular Imaging</topic><topic>Permeability - radiation effects</topic><topic>Radiation damage</topic><topic>Radiation dosage</topic><topic>Radiotherapy</topic><topic>REGULAR ARTICLES</topic><topic>Space life sciences</topic><topic>Statistical significance</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Young, Erik F.</creatorcontrib><creatorcontrib>Smilenov, Lubomir B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Young, Erik F.</au><au>Smilenov, Lubomir B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>176</volume><issue>4</issue><spage>415</spage><epage>424</epage><pages>415-424</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported.</abstract><cop>United States</cop><pub>The Radiation Research Society</pub><pmid>21861763</pmid><doi>10.1667/RR2665.1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Radiation research, 2011-10, Vol.176 (4), p.415-424 |
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| subjects | Cell Movement - radiation effects Coronary Vessels - cytology Cytoskeleton - radiation effects Electric Impedance Electrodes Endothelial cells Endothelium Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Endothelium, Vascular - radiation effects Humans Ionizing radiation Irradiation Kinetics Models, Biological Molecular Imaging Permeability - radiation effects Radiation damage Radiation dosage Radiotherapy REGULAR ARTICLES Space life sciences Statistical significance Time Factors |
| title | Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation |
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