Functional Screening in the Melanophore Bioassay
The melanophore bioassay is a robust, sensitive, and versatile procedure for screening G protein–coupled receptors in a variety of formats. Because melanophores contain a wide variety of G proteins, they can be employed as a sensitive, real‐time response system for studying transfected receptors and...
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| Veröffentlicht in: | Current Protocols in Pharmacology 2005-07, Vol.29 (1), p.12.9.1-12.9.16 |
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| container_end_page | 12.9.16 |
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| container_issue | 1 |
| container_start_page | 12.9.1 |
| container_title | Current Protocols in Pharmacology |
| container_volume | 29 |
| creator | Jayawickreme, Channa Sauls, Howard Watson, Chris Moncol, David Rimele, Thomas Kenakin, Terry |
| description | The melanophore bioassay is a robust, sensitive, and versatile procedure for screening G
protein–coupled receptors in a variety of formats. Because melanophores contain a wide variety of G
proteins, they can be employed as a sensitive, real‐time response system for studying transfected
receptors and for defining equilibria for drug effects. This assay can be run in 96‐well microtiter
plates or in open‐lawn 1536 format, and can yield conventional agonist‐antagonist as well as
constitutive assays. |
| doi_str_mv | 10.1002/0471141755.ph1209s29 |
| format | Article |
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receptors and for defining equilibria for drug effects. This assay can be run in 96‐well microtiter
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protein–coupled receptors in a variety of formats. Because melanophores contain a wide variety of G
proteins, they can be employed as a sensitive, real‐time response system for studying transfected
receptors and for defining equilibria for drug effects. This assay can be run in 96‐well microtiter
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| fulltext | fulltext |
| identifier | ISSN: 1934-8282 |
| ispartof | Current Protocols in Pharmacology, 2005-07, Vol.29 (1), p.12.9.1-12.9.16 |
| issn | 1934-8282 1934-8290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_894814281 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Line constitutive screening Drug Evaluation, Preclinical - methods G‐protein coupled receptors high throughput screening melanophore Melanophores - drug effects Melanophores - physiology Receptors, G-Protein-Coupled - physiology Xenopus laevis |
| title | Functional Screening in the Melanophore Bioassay |
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