Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay
Here, a three-dimensional (3D) carbohydrate microarray-based plasmon resonance light scattering (RLS) assay has been established for studying carbohydrate-lectin binding with high selectivity. The 3D carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made...
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| Veröffentlicht in: | Analyst (London) 2011-10, Vol.136 (20), p.4301-4307 |
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| creator | Li, Xiaomei Gao, Jingqing Liu, Dianjun Wang, Zhenxin |
| description | Here, a three-dimensional (3D) carbohydrate microarray-based plasmon resonance light scattering (RLS) assay has been established for studying carbohydrate-lectin binding with high selectivity. The 3D carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made fourth-generation (G4) NH(2)-terminated poly(amidoamine) dendrimers (PAMAM)-modified substrate. After marking the carbohydrate-lectin binding events by 13 nm peptide-stabilized gold nanoparticles through the biotin-avidin reaction, the 3D microarray can be directly detected by the RLS scanner without the conventional silver enhancement step. The well defined recognition systems: three monosaccharides (Man-α, Glc-α and Gal-β) with two lectins (Con A and RCA 120), have been chosen here to establish the RLS assay, respectively. Quantitative determination of the surface dissociation constants (K(D,surf)) for surface carbohydrates and lectins has been achieved. In addition, inhibition values (i.e. the inhibition constants (K(i)) and the concentrations of inhibitors required to produce 50% inhibition (IC(50))) for inhibitors in solution are also demonstrated by the saccharide competing assays. |
| doi_str_mv | 10.1039/c1an15230k |
| format | Article |
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The 3D carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made fourth-generation (G4) NH(2)-terminated poly(amidoamine) dendrimers (PAMAM)-modified substrate. After marking the carbohydrate-lectin binding events by 13 nm peptide-stabilized gold nanoparticles through the biotin-avidin reaction, the 3D microarray can be directly detected by the RLS scanner without the conventional silver enhancement step. The well defined recognition systems: three monosaccharides (Man-α, Glc-α and Gal-β) with two lectins (Con A and RCA 120), have been chosen here to establish the RLS assay, respectively. Quantitative determination of the surface dissociation constants (K(D,surf)) for surface carbohydrates and lectins has been achieved. In addition, inhibition values (i.e. the inhibition constants (K(i)) and the concentrations of inhibitors required to produce 50% inhibition (IC(50))) for inhibitors in solution are also demonstrated by the saccharide competing assays.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c1an15230k</identifier><identifier>PMID: 21887418</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical chemistry ; Avidin - chemistry ; Avidin - metabolism ; Biotin - chemistry ; Biotin - metabolism ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Concanavalin A - analysis ; Concanavalin A - metabolism ; Dendrimers - chemistry ; Exact sciences and technology ; Gas chromatographic methods ; General, instrumentation ; Gold - chemistry ; Indexing in process ; Lectins - analysis ; Lectins - metabolism ; Light ; Metal Nanoparticles - chemistry ; Microarray Analysis ; Monosaccharides - chemistry ; Monosaccharides - metabolism ; Plant Lectins - analysis ; Plant Lectins - metabolism ; Protein Binding ; Scattering, Radiation ; Spectrometric and optical methods</subject><ispartof>Analyst (London), 2011-10, Vol.136 (20), p.4301-4307</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-3c637300fa98ae2cc1e71d7bb1cfdaf82157d5e1e858ac225952277360bfd0b53</citedby><cites>FETCH-LOGICAL-c349t-3c637300fa98ae2cc1e71d7bb1cfdaf82157d5e1e858ac225952277360bfd0b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24586151$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21887418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiaomei</creatorcontrib><creatorcontrib>Gao, Jingqing</creatorcontrib><creatorcontrib>Liu, Dianjun</creatorcontrib><creatorcontrib>Wang, Zhenxin</creatorcontrib><title>Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Here, a three-dimensional (3D) carbohydrate microarray-based plasmon resonance light scattering (RLS) assay has been established for studying carbohydrate-lectin binding with high selectivity. The 3D carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made fourth-generation (G4) NH(2)-terminated poly(amidoamine) dendrimers (PAMAM)-modified substrate. After marking the carbohydrate-lectin binding events by 13 nm peptide-stabilized gold nanoparticles through the biotin-avidin reaction, the 3D microarray can be directly detected by the RLS scanner without the conventional silver enhancement step. The well defined recognition systems: three monosaccharides (Man-α, Glc-α and Gal-β) with two lectins (Con A and RCA 120), have been chosen here to establish the RLS assay, respectively. Quantitative determination of the surface dissociation constants (K(D,surf)) for surface carbohydrates and lectins has been achieved. In addition, inhibition values (i.e. the inhibition constants (K(i)) and the concentrations of inhibitors required to produce 50% inhibition (IC(50))) for inhibitors in solution are also demonstrated by the saccharide competing assays.</description><subject>Analytical chemistry</subject><subject>Avidin - chemistry</subject><subject>Avidin - metabolism</subject><subject>Biotin - chemistry</subject><subject>Biotin - metabolism</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Concanavalin A - analysis</subject><subject>Concanavalin A - metabolism</subject><subject>Dendrimers - chemistry</subject><subject>Exact sciences and technology</subject><subject>Gas chromatographic methods</subject><subject>General, instrumentation</subject><subject>Gold - chemistry</subject><subject>Indexing in process</subject><subject>Lectins - analysis</subject><subject>Lectins - metabolism</subject><subject>Light</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microarray Analysis</subject><subject>Monosaccharides - chemistry</subject><subject>Monosaccharides - metabolism</subject><subject>Plant Lectins - analysis</subject><subject>Plant Lectins - metabolism</subject><subject>Protein Binding</subject><subject>Scattering, Radiation</subject><subject>Spectrometric and optical methods</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90cGO1iAQB3BiNO63qxcfwPRiNCZVBkpLj2ajq8kmHtRzM4VhP7QtFeihD-L7yrqf7s0LBPLLwMyfsWfA3wCX_VsDuIASkv94wA4g26ZWSuiH7MA5l7VoVXPGzlP6Xo7AFX_MzgRo3TWgD-zXl7zZ3S83VT5S5ZdMEU32YamCqwzGMRx3GzFTvcaQyZf75Q-1tNjoZ4r1HKx3nmyVwuTLuq1riLka92r2JgaMEfd6xFTEOmGaS4FIKSy4GKomf3PMVTKYy8u338CUcH_CHjmcEj097Rfs24f3Xy8_1tefrz5dvruujWz6XEvTyk5y7rDXSMIYoA5sN45gnEWnBajOKgLSSqMRQvVKiK6TLR-d5aOSF-zlXd3S3M-NUh5mnwxNEy4UtjTovtEgWtBFvvqvBN720De8k4W-vqOl-ZQiuWEtg8K4FzTcBjbcB1bw81PdbZzJ_qN_EyrgxQlgmdLkYhmbT_euUboFBfI3rnShwA</recordid><startdate>20111021</startdate><enddate>20111021</enddate><creator>Li, Xiaomei</creator><creator>Gao, Jingqing</creator><creator>Liu, Dianjun</creator><creator>Wang, Zhenxin</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7UA</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20111021</creationdate><title>Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay</title><author>Li, Xiaomei ; Gao, Jingqing ; Liu, Dianjun ; Wang, Zhenxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-3c637300fa98ae2cc1e71d7bb1cfdaf82157d5e1e858ac225952277360bfd0b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Avidin - chemistry</topic><topic>Avidin - metabolism</topic><topic>Biotin - chemistry</topic><topic>Biotin - metabolism</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Concanavalin A - analysis</topic><topic>Concanavalin A - metabolism</topic><topic>Dendrimers - chemistry</topic><topic>Exact sciences and technology</topic><topic>Gas chromatographic methods</topic><topic>General, instrumentation</topic><topic>Gold - chemistry</topic><topic>Indexing in process</topic><topic>Lectins - analysis</topic><topic>Lectins - metabolism</topic><topic>Light</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microarray Analysis</topic><topic>Monosaccharides - chemistry</topic><topic>Monosaccharides - metabolism</topic><topic>Plant Lectins - analysis</topic><topic>Plant Lectins - metabolism</topic><topic>Protein Binding</topic><topic>Scattering, Radiation</topic><topic>Spectrometric and optical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaomei</creatorcontrib><creatorcontrib>Gao, Jingqing</creatorcontrib><creatorcontrib>Liu, Dianjun</creatorcontrib><creatorcontrib>Wang, Zhenxin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaomei</au><au>Gao, Jingqing</au><au>Liu, Dianjun</au><au>Wang, Zhenxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2011-10-21</date><risdate>2011</risdate><volume>136</volume><issue>20</issue><spage>4301</spage><epage>4307</epage><pages>4301-4307</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>Here, a three-dimensional (3D) carbohydrate microarray-based plasmon resonance light scattering (RLS) assay has been established for studying carbohydrate-lectin binding with high selectivity. The 3D carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made fourth-generation (G4) NH(2)-terminated poly(amidoamine) dendrimers (PAMAM)-modified substrate. After marking the carbohydrate-lectin binding events by 13 nm peptide-stabilized gold nanoparticles through the biotin-avidin reaction, the 3D microarray can be directly detected by the RLS scanner without the conventional silver enhancement step. The well defined recognition systems: three monosaccharides (Man-α, Glc-α and Gal-β) with two lectins (Con A and RCA 120), have been chosen here to establish the RLS assay, respectively. Quantitative determination of the surface dissociation constants (K(D,surf)) for surface carbohydrates and lectins has been achieved. In addition, inhibition values (i.e. the inhibition constants (K(i)) and the concentrations of inhibitors required to produce 50% inhibition (IC(50))) for inhibitors in solution are also demonstrated by the saccharide competing assays.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>21887418</pmid><doi>10.1039/c1an15230k</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry Avidin - chemistry Avidin - metabolism Biotin - chemistry Biotin - metabolism Chemistry Chromatographic methods and physical methods associated with chromatography Concanavalin A - analysis Concanavalin A - metabolism Dendrimers - chemistry Exact sciences and technology Gas chromatographic methods General, instrumentation Gold - chemistry Indexing in process Lectins - analysis Lectins - metabolism Light Metal Nanoparticles - chemistry Microarray Analysis Monosaccharides - chemistry Monosaccharides - metabolism Plant Lectins - analysis Plant Lectins - metabolism Protein Binding Scattering, Radiation Spectrometric and optical methods |
| title | Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay |
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