Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase
► Super paramagnetic nanoparticles were prepared by a surface-initiated radical polymerization. ► This nanoparticle was provided with positive charge and active epoxy groups simultaneously. ► The candida rugosa lipase (CRL) was immobilized via electrostatic adsorption and covalent binding. ► The lip...
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| Veröffentlicht in: | Biochemical engineering journal 2011-10, Vol.56 (3), p.142-149 |
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| creator | Liu, Xiao Lei, Lin Li, Yanfeng Zhu, Hao Cui, Yanjun Hu, Huaiyuan |
| description | ► Super paramagnetic nanoparticles were prepared by a surface-initiated radical polymerization. ► This nanoparticle was provided with positive charge and active epoxy groups simultaneously. ► The candida rugosa lipase (CRL) was immobilized via electrostatic adsorption and covalent binding. ► The lipase loading amount was 68.3
±
0.5
mg CRL/g support and the activity recovery reached to 60.4% (±1.6%).
The super paramagnetic nanoparticles Fe
3O
4/(2-hydroxyethty methacrylate-co-dimethyl dially ammonium chloride) copolymer, i.e. Fe
3O
4/P(HEMA–DMDAAC), with positive charge and active epoxy groups simultaneously, were synthesized by a surface-initiated radical polymerization and activation reaction with epoxy chloropropane. The production particles were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). It was verified that the magnetic microspheres held small diameters of 80–100
nm and displayed super paramagnetic property with saturation magnetization of 38.9
emu/g. The candida rugosa lipase (CRL), meanwhile, was immobilized onto the magnetic microspheres via electrostatic adsorption and covalent binding, the loading amount of lipase was 68.3
±
0.5
mg CRL/g support and the activity recovery of the obtained immobilized lipase reached to 60.4% (±1.6%). |
| doi_str_mv | 10.1016/j.bej.2011.05.013 |
| format | Article |
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±
0.5
mg CRL/g support and the activity recovery reached to 60.4% (±1.6%).
The super paramagnetic nanoparticles Fe
3O
4/(2-hydroxyethty methacrylate-co-dimethyl dially ammonium chloride) copolymer, i.e. Fe
3O
4/P(HEMA–DMDAAC), with positive charge and active epoxy groups simultaneously, were synthesized by a surface-initiated radical polymerization and activation reaction with epoxy chloropropane. The production particles were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). It was verified that the magnetic microspheres held small diameters of 80–100
nm and displayed super paramagnetic property with saturation magnetization of 38.9
emu/g. The candida rugosa lipase (CRL), meanwhile, was immobilized onto the magnetic microspheres via electrostatic adsorption and covalent binding, the loading amount of lipase was 68.3
±
0.5
mg CRL/g support and the activity recovery of the obtained immobilized lipase reached to 60.4% (±1.6%).</description><identifier>ISSN: 1369-703X</identifier><identifier>EISSN: 1873-295X</identifier><identifier>DOI: 10.1016/j.bej.2011.05.013</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>adsorption ; ammonium chloride ; Biological and medical sciences ; Biotechnology ; Candida rugosa ; composite polymers ; Covalent binding ; Electrostatic adsorption ; Enzyme technology ; epoxides ; Fourier transform infrared spectroscopy ; Fundamental and applied biological sciences. Psychology ; General aspects ; Immobilization techniques ; Immobilized CRL ; Methods. Procedures. Technologies ; nanoparticles ; polymerization ; Reusability ; Super paramagnetism ; thermogravimetry ; transmission electron microscopy</subject><ispartof>Biochemical engineering journal, 2011-10, Vol.56 (3), p.142-149</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-5ce5030b1a27845642aee9c4d0977633fc7e737701a21e97792612d84623a6213</citedby><cites>FETCH-LOGICAL-c383t-5ce5030b1a27845642aee9c4d0977633fc7e737701a21e97792612d84623a6213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bej.2011.05.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24488424$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiao</creatorcontrib><creatorcontrib>Lei, Lin</creatorcontrib><creatorcontrib>Li, Yanfeng</creatorcontrib><creatorcontrib>Zhu, Hao</creatorcontrib><creatorcontrib>Cui, Yanjun</creatorcontrib><creatorcontrib>Hu, Huaiyuan</creatorcontrib><title>Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase</title><title>Biochemical engineering journal</title><description>► Super paramagnetic nanoparticles were prepared by a surface-initiated radical polymerization. ► This nanoparticle was provided with positive charge and active epoxy groups simultaneously. ► The candida rugosa lipase (CRL) was immobilized via electrostatic adsorption and covalent binding. ► The lipase loading amount was 68.3
±
0.5
mg CRL/g support and the activity recovery reached to 60.4% (±1.6%).
The super paramagnetic nanoparticles Fe
3O
4/(2-hydroxyethty methacrylate-co-dimethyl dially ammonium chloride) copolymer, i.e. Fe
3O
4/P(HEMA–DMDAAC), with positive charge and active epoxy groups simultaneously, were synthesized by a surface-initiated radical polymerization and activation reaction with epoxy chloropropane. The production particles were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). It was verified that the magnetic microspheres held small diameters of 80–100
nm and displayed super paramagnetic property with saturation magnetization of 38.9
emu/g. The candida rugosa lipase (CRL), meanwhile, was immobilized onto the magnetic microspheres via electrostatic adsorption and covalent binding, the loading amount of lipase was 68.3
±
0.5
mg CRL/g support and the activity recovery of the obtained immobilized lipase reached to 60.4% (±1.6%).</description><subject>adsorption</subject><subject>ammonium chloride</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Candida rugosa</subject><subject>composite polymers</subject><subject>Covalent binding</subject><subject>Electrostatic adsorption</subject><subject>Enzyme technology</subject><subject>epoxides</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Immobilization techniques</subject><subject>Immobilized CRL</subject><subject>Methods. Procedures. Technologies</subject><subject>nanoparticles</subject><subject>polymerization</subject><subject>Reusability</subject><subject>Super paramagnetism</subject><subject>thermogravimetry</subject><subject>transmission electron microscopy</subject><issn>1369-703X</issn><issn>1873-295X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhk1oIWmSH5BTdSk92dGXJZueSkiTQKCFZmFvYlYeL1psy5WcQPLrO4tDjz3NMDzzzvAUxZXgleDCXB-qHR4qyYWoeF1xoU6KM9FYVcq23n6gXpm2tFxtT4tPOR8450ZZe1bgr4QzJFhCnFjsmYeUAqbMdpCxYzQcYT_hEjybYIqEUjtgZvsE_ULEHIfXERODqWNhHOMuDOFtjetjYkOYKeii-NjDkPHyvZ4Xmx-3Tzf35ePPu4eb74-lV41aytpjzRXfCZC20bXREhBbrzveWmuU6r1FS29zAgTSrJVGyK7RRiowUqjz4uuaO6f45xnz4saQPQ4DTBifs2taRclSSCLFSvoUc07YuzmFEdKrE9wdjbqDI6PuaNTx2pFR2vnyng7Zw9AnmHzI_xal1k2jpSbu88r1EB3sEzGb3xRkyHqtrD5e_7YSSDJeyLfLPuDksQsJ_eK6GP7zx1_5iJS5</recordid><startdate>20111015</startdate><enddate>20111015</enddate><creator>Liu, Xiao</creator><creator>Lei, Lin</creator><creator>Li, Yanfeng</creator><creator>Zhu, Hao</creator><creator>Cui, Yanjun</creator><creator>Hu, Huaiyuan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20111015</creationdate><title>Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase</title><author>Liu, Xiao ; Lei, Lin ; Li, Yanfeng ; Zhu, Hao ; Cui, Yanjun ; Hu, Huaiyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-5ce5030b1a27845642aee9c4d0977633fc7e737701a21e97792612d84623a6213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adsorption</topic><topic>ammonium chloride</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Candida rugosa</topic><topic>composite polymers</topic><topic>Covalent binding</topic><topic>Electrostatic adsorption</topic><topic>Enzyme technology</topic><topic>epoxides</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Immobilization techniques</topic><topic>Immobilized CRL</topic><topic>Methods. Procedures. Technologies</topic><topic>nanoparticles</topic><topic>polymerization</topic><topic>Reusability</topic><topic>Super paramagnetism</topic><topic>thermogravimetry</topic><topic>transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiao</creatorcontrib><creatorcontrib>Lei, Lin</creatorcontrib><creatorcontrib>Li, Yanfeng</creatorcontrib><creatorcontrib>Zhu, Hao</creatorcontrib><creatorcontrib>Cui, Yanjun</creatorcontrib><creatorcontrib>Hu, Huaiyuan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biochemical engineering journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiao</au><au>Lei, Lin</au><au>Li, Yanfeng</au><au>Zhu, Hao</au><au>Cui, Yanjun</au><au>Hu, Huaiyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase</atitle><jtitle>Biochemical engineering journal</jtitle><date>2011-10-15</date><risdate>2011</risdate><volume>56</volume><issue>3</issue><spage>142</spage><epage>149</epage><pages>142-149</pages><issn>1369-703X</issn><eissn>1873-295X</eissn><abstract>► Super paramagnetic nanoparticles were prepared by a surface-initiated radical polymerization. ► This nanoparticle was provided with positive charge and active epoxy groups simultaneously. ► The candida rugosa lipase (CRL) was immobilized via electrostatic adsorption and covalent binding. ► The lipase loading amount was 68.3
±
0.5
mg CRL/g support and the activity recovery reached to 60.4% (±1.6%).
The super paramagnetic nanoparticles Fe
3O
4/(2-hydroxyethty methacrylate-co-dimethyl dially ammonium chloride) copolymer, i.e. Fe
3O
4/P(HEMA–DMDAAC), with positive charge and active epoxy groups simultaneously, were synthesized by a surface-initiated radical polymerization and activation reaction with epoxy chloropropane. The production particles were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). It was verified that the magnetic microspheres held small diameters of 80–100
nm and displayed super paramagnetic property with saturation magnetization of 38.9
emu/g. The candida rugosa lipase (CRL), meanwhile, was immobilized onto the magnetic microspheres via electrostatic adsorption and covalent binding, the loading amount of lipase was 68.3
±
0.5
mg CRL/g support and the activity recovery of the obtained immobilized lipase reached to 60.4% (±1.6%).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.bej.2011.05.013</doi><tpages>8</tpages></addata></record> |
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| subjects | adsorption ammonium chloride Biological and medical sciences Biotechnology Candida rugosa composite polymers Covalent binding Electrostatic adsorption Enzyme technology epoxides Fourier transform infrared spectroscopy Fundamental and applied biological sciences. Psychology General aspects Immobilization techniques Immobilized CRL Methods. Procedures. Technologies nanoparticles polymerization Reusability Super paramagnetism thermogravimetry transmission electron microscopy |
| title | Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase |
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