A new purification process for goose immunoglobulin IgY(ΔFc) with hydrophobic charge-induction chromatography
[Display omitted] ► Novel bioseparation technology, hydrophobic charge-induction chromatography, was applied to separate IgY(ΔFc). ► The adsorption isotherms of IgY(ΔFc) onto new HCIC adsorbent (MMI-Bestarose) were investigated. ► The HCIC separation conditions were optimized and high separation eff...
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| Veröffentlicht in: | Biochemical engineering journal 2011-10, Vol.56 (3), p.205-211 |
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| creator | Tong, Hong-Fei Lin, Dong-Qiang Pan, Yue Yao, Shan-Jing |
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► Novel bioseparation technology, hydrophobic charge-induction chromatography, was applied to separate IgY(ΔFc). ► The adsorption isotherms of IgY(ΔFc) onto new HCIC adsorbent (MMI-Bestarose) were investigated. ► The HCIC separation conditions were optimized and high separation efficiency was obtained. ► New process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.
Hydrophobic charge-induction chromatography (HCIC) is a novel bioseparation technique, especially for antibody purification. In this study, HCIC was used for the purification of goose immunoglobulin IgY(ΔFc). IgY(ΔFc) is a kind of unique immunoglobulin existing in waterfowl, which natively lacks C
H3 and C
H4 domains of heavy chains and has some particular therapeutic applications. A new HCIC ligand, 2-mercapto-1-methyl-imidazole (MMI), was coupled to the divinyl-sulfone-activated agarose matrix to prepare the HCIC gel. The adsorption isotherms of IgY(ΔFc) were investigated at different pHs. The saturated capacity of IgY(ΔFc) at pH 5.0 could reach 187.5
mg/ml gel, and the pH-dependent adsorption behaviors were found. After the goose plasma was pre-treated with caprylic acid to precipitate some impurities, the supernatant could be directly loaded onto the HCIC column for IgY(ΔFc) separation. The operation conditions were optimized, including the loading pH, elution pH and the loading volume. High separation efficiency was obtained with final purity of 98.6% and the yield of 85.0%. The results indicate that the new process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma. |
| doi_str_mv | 10.1016/j.bej.2011.06.010 |
| format | Article |
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► Novel bioseparation technology, hydrophobic charge-induction chromatography, was applied to separate IgY(ΔFc). ► The adsorption isotherms of IgY(ΔFc) onto new HCIC adsorbent (MMI-Bestarose) were investigated. ► The HCIC separation conditions were optimized and high separation efficiency was obtained. ► New process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.
Hydrophobic charge-induction chromatography (HCIC) is a novel bioseparation technique, especially for antibody purification. In this study, HCIC was used for the purification of goose immunoglobulin IgY(ΔFc). IgY(ΔFc) is a kind of unique immunoglobulin existing in waterfowl, which natively lacks C
H3 and C
H4 domains of heavy chains and has some particular therapeutic applications. A new HCIC ligand, 2-mercapto-1-methyl-imidazole (MMI), was coupled to the divinyl-sulfone-activated agarose matrix to prepare the HCIC gel. The adsorption isotherms of IgY(ΔFc) were investigated at different pHs. The saturated capacity of IgY(ΔFc) at pH 5.0 could reach 187.5
mg/ml gel, and the pH-dependent adsorption behaviors were found. After the goose plasma was pre-treated with caprylic acid to precipitate some impurities, the supernatant could be directly loaded onto the HCIC column for IgY(ΔFc) separation. The operation conditions were optimized, including the loading pH, elution pH and the loading volume. High separation efficiency was obtained with final purity of 98.6% and the yield of 85.0%. The results indicate that the new process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.</description><identifier>ISSN: 1369-703X</identifier><identifier>EISSN: 1873-295X</identifier><identifier>DOI: 10.1016/j.bej.2011.06.010</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>adsorption ; agarose ; antibodies ; Biological and medical sciences ; Biotechnology ; chromatography ; cost effectiveness ; Fundamental and applied biological sciences. Psychology ; geese ; gels ; Goose plasma ; Hydrophobic charge-induction chromatography ; hydrophobicity ; Immunoglobulin ; immunoglobulin Y ; methane ; octanoic acid ; Purification ; purification methods ; sorption isotherms ; waterfowl</subject><ispartof>Biochemical engineering journal, 2011-10, Vol.56 (3), p.205-211</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-4e45bcdf0c00cecbb806a1547937b47c2e8c857a7e1fbb25c15faf917b8beb773</citedby><cites>FETCH-LOGICAL-c383t-4e45bcdf0c00cecbb806a1547937b47c2e8c857a7e1fbb25c15faf917b8beb773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bej.2011.06.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24488432$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, Hong-Fei</creatorcontrib><creatorcontrib>Lin, Dong-Qiang</creatorcontrib><creatorcontrib>Pan, Yue</creatorcontrib><creatorcontrib>Yao, Shan-Jing</creatorcontrib><title>A new purification process for goose immunoglobulin IgY(ΔFc) with hydrophobic charge-induction chromatography</title><title>Biochemical engineering journal</title><description>[Display omitted]
► Novel bioseparation technology, hydrophobic charge-induction chromatography, was applied to separate IgY(ΔFc). ► The adsorption isotherms of IgY(ΔFc) onto new HCIC adsorbent (MMI-Bestarose) were investigated. ► The HCIC separation conditions were optimized and high separation efficiency was obtained. ► New process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.
Hydrophobic charge-induction chromatography (HCIC) is a novel bioseparation technique, especially for antibody purification. In this study, HCIC was used for the purification of goose immunoglobulin IgY(ΔFc). IgY(ΔFc) is a kind of unique immunoglobulin existing in waterfowl, which natively lacks C
H3 and C
H4 domains of heavy chains and has some particular therapeutic applications. A new HCIC ligand, 2-mercapto-1-methyl-imidazole (MMI), was coupled to the divinyl-sulfone-activated agarose matrix to prepare the HCIC gel. The adsorption isotherms of IgY(ΔFc) were investigated at different pHs. The saturated capacity of IgY(ΔFc) at pH 5.0 could reach 187.5
mg/ml gel, and the pH-dependent adsorption behaviors were found. After the goose plasma was pre-treated with caprylic acid to precipitate some impurities, the supernatant could be directly loaded onto the HCIC column for IgY(ΔFc) separation. The operation conditions were optimized, including the loading pH, elution pH and the loading volume. High separation efficiency was obtained with final purity of 98.6% and the yield of 85.0%. The results indicate that the new process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.</description><subject>adsorption</subject><subject>agarose</subject><subject>antibodies</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>chromatography</subject><subject>cost effectiveness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>geese</subject><subject>gels</subject><subject>Goose plasma</subject><subject>Hydrophobic charge-induction chromatography</subject><subject>hydrophobicity</subject><subject>Immunoglobulin</subject><subject>immunoglobulin Y</subject><subject>methane</subject><subject>octanoic acid</subject><subject>Purification</subject><subject>purification methods</subject><subject>sorption isotherms</subject><subject>waterfowl</subject><issn>1369-703X</issn><issn>1873-295X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kMFu1TAQRSMEEqXwAazwBkEXCXac2H5iVVUUKlViAZXKyrIn48RPSRzshOr9B9_Vb8LlVSxZzSzOvZo5RfGa0YpRJj7sK4v7qqaMVVRUlNEnxQlTkpf1rr19mncudqWk_PZ58SKlPaVUcClPivmczHhHli1658GsPsxkiQEwJeJCJH0ICYmfpm0O_RjsNvqZXPU_3t__voQzcufXgQyHLoZlCNYDgcHEHks_dxv8LYMhhsmsoY9mGQ4vi2fOjAlfPc7T4uby0_eLL-X1189XF-fXJXDF17LBprXQOQqUAoK1igrD2kbuuLSNhBoVqFYaicxZW7fAWmfcjkmrLFop-Wnx7tibf_m5YVr15BPgOJoZw5a02vFaKCHaTLIjCTGkFNHpJfrJxINmVD-o1Xud1eoHtZoKndXmzNvHdpPAjC6aGXz6F6ybRqmG15l7c-ScCdr0MTM333KRyPpbLjnPxMcjgVnGL49RJ_A4A3Y-Iqy6C_4_d_wBP0Oa9Q</recordid><startdate>20111015</startdate><enddate>20111015</enddate><creator>Tong, Hong-Fei</creator><creator>Lin, Dong-Qiang</creator><creator>Pan, Yue</creator><creator>Yao, Shan-Jing</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20111015</creationdate><title>A new purification process for goose immunoglobulin IgY(ΔFc) with hydrophobic charge-induction chromatography</title><author>Tong, Hong-Fei ; Lin, Dong-Qiang ; Pan, Yue ; Yao, Shan-Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-4e45bcdf0c00cecbb806a1547937b47c2e8c857a7e1fbb25c15faf917b8beb773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adsorption</topic><topic>agarose</topic><topic>antibodies</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>chromatography</topic><topic>cost effectiveness</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>geese</topic><topic>gels</topic><topic>Goose plasma</topic><topic>Hydrophobic charge-induction chromatography</topic><topic>hydrophobicity</topic><topic>Immunoglobulin</topic><topic>immunoglobulin Y</topic><topic>methane</topic><topic>octanoic acid</topic><topic>Purification</topic><topic>purification methods</topic><topic>sorption isotherms</topic><topic>waterfowl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, Hong-Fei</creatorcontrib><creatorcontrib>Lin, Dong-Qiang</creatorcontrib><creatorcontrib>Pan, Yue</creatorcontrib><creatorcontrib>Yao, Shan-Jing</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biochemical engineering journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, Hong-Fei</au><au>Lin, Dong-Qiang</au><au>Pan, Yue</au><au>Yao, Shan-Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new purification process for goose immunoglobulin IgY(ΔFc) with hydrophobic charge-induction chromatography</atitle><jtitle>Biochemical engineering journal</jtitle><date>2011-10-15</date><risdate>2011</risdate><volume>56</volume><issue>3</issue><spage>205</spage><epage>211</epage><pages>205-211</pages><issn>1369-703X</issn><eissn>1873-295X</eissn><abstract>[Display omitted]
► Novel bioseparation technology, hydrophobic charge-induction chromatography, was applied to separate IgY(ΔFc). ► The adsorption isotherms of IgY(ΔFc) onto new HCIC adsorbent (MMI-Bestarose) were investigated. ► The HCIC separation conditions were optimized and high separation efficiency was obtained. ► New process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.
Hydrophobic charge-induction chromatography (HCIC) is a novel bioseparation technique, especially for antibody purification. In this study, HCIC was used for the purification of goose immunoglobulin IgY(ΔFc). IgY(ΔFc) is a kind of unique immunoglobulin existing in waterfowl, which natively lacks C
H3 and C
H4 domains of heavy chains and has some particular therapeutic applications. A new HCIC ligand, 2-mercapto-1-methyl-imidazole (MMI), was coupled to the divinyl-sulfone-activated agarose matrix to prepare the HCIC gel. The adsorption isotherms of IgY(ΔFc) were investigated at different pHs. The saturated capacity of IgY(ΔFc) at pH 5.0 could reach 187.5
mg/ml gel, and the pH-dependent adsorption behaviors were found. After the goose plasma was pre-treated with caprylic acid to precipitate some impurities, the supernatant could be directly loaded onto the HCIC column for IgY(ΔFc) separation. The operation conditions were optimized, including the loading pH, elution pH and the loading volume. High separation efficiency was obtained with final purity of 98.6% and the yield of 85.0%. The results indicate that the new process with HCIC can be a promising technology for the cost-effective separation of IgY(ΔFc) from plasma.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.bej.2011.06.010</doi><tpages>7</tpages></addata></record> |
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| subjects | adsorption agarose antibodies Biological and medical sciences Biotechnology chromatography cost effectiveness Fundamental and applied biological sciences. Psychology geese gels Goose plasma Hydrophobic charge-induction chromatography hydrophobicity Immunoglobulin immunoglobulin Y methane octanoic acid Purification purification methods sorption isotherms waterfowl |
| title | A new purification process for goose immunoglobulin IgY(ΔFc) with hydrophobic charge-induction chromatography |
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