Reducing chimera formation during PCR amplification to ensure accurate genotyping

Measurements of population diversity are fundamental to the reconstruction of the evolutionary and epidemiological history of organisms. Commonly used protocols to measure population diversity using the polymerase chain reaction (PCR) are prone to the introduction of artificial chimeras. These are o...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Gene 2010-12, Vol.469 (1), p.45-51
Hauptverfasser:	Smyth, R.P., Schlub, T.E., Grimm, A., Venturi, V., Chopra, A., Mallal, S., Davenport, M.P., Mak, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chimeras Genetics, Population Genotype Genotyping PCR artifact Plasmids Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Population genetics Recombination Recombination, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!