Isolation of detergent-resistant membranes from plant photosynthetic and non-photosynthetic tissues

Microdomains, or lipid rafts, are transient membrane regions enriched in sphingolipids and sterols that have only recently, but intensively, been studied in plants. In this work, we report a detailed, easy-to-follow, and fast procedure to isolate detergent-resistant membranes (DRMs) from purified pl...

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Veröffentlicht in:	Analytical biochemistry 2011-10, Vol.417 (2), p.220-227
Hauptverfasser:	Carmona-Salazar, Laura, El Hafidi, Mohammed, Enríquez-Arredondo, Consuelo, Vázquez-Vázquez, Christian, González de la Vara, Luis E., Gavilanes-Ruíz, Marina
Format:	Artikel
Sprache:	eng
Schlagworte:	corn Detergent-resistant membranes Detergents - chemistry developmental stages eukaryotic cells Isolation of detergent-resistant membranes leaves Lipid rafts Membrane microdomains Membrane Microdomains - chemistry Membrane Microdomains - ultrastructure Nicotiana - chemistry Nicotiana - ultrastructure Nicotiana tabacum Phaseolus - chemistry Phaseolus - ultrastructure Phaseolus vulgaris Photosynthesis Plant membrane microdomains plasma membrane Seeds - chemistry Seeds - ultrastructure sphingolipids sterols Sterols - analysis Sterols - chemistry tissues ultrastructure Zea mays Zea mays - chemistry Zea mays - ultrastructure
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