Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome
BACKGROUND Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysi...
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| Veröffentlicht in: | Human reproduction (Oxford) 2011-10, Vol.26 (10), p.2869-2873 |
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| creator | Seow, Kok-Min Hwang, Jiann-Loung Wang, Peng-Hui Ho, Low-Tone Juan, Chi-Chang |
| description | BACKGROUND
Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysis of gene expression. The aims of the study were to compare the expression of visfatin in PBMCs with that in omental adipose tissue in women with polycystic ovary syndrome (PCOS).
METHODS
Visfatin mRNA was measured in omental adipose tissue and PBMCs from 10 women with PCOS and 10 healthy controls, matched for BMI and age, using the real-time polymerase chain reaction (PCR).
RESULTS
The expression of visfatin mRNA in both omental adipose tissue and PBMCs from the women with PCOS was significantly higher (P= 0.01 and P= 0.05, respectively) than that in the controls. This finding indicated that mononuclear cells are a potential source of visfatin in women with PCOS. However, only the expression of visfatin mRNA in adipose tissue, not that in PBMCs, showed a significant positive correlation with insulin levels 2h after glucose loading (P= 0.044, r2 = 0.45), and with homeostasis model assessment–insulin resistance (HOMAIR; P= 0.035, r2 = 0.47). In addition, the expression of visfatin mRNA in PBMCs did not correlate with the expression of visfatin mRNA in omental adipose tissue.
CONCLUSIONS
PCOS is associated with increased visfatin mRNA concentrations in PBMCs and in omental adipose tissue. However, only visfatin mRNA concentration in omental adipose tissue is closely correlated with BMI and insulin resistance. |
| doi_str_mv | 10.1093/humrep/der267 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_890675305</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/humrep/der267</oup_id><sourcerecordid>1093442427</sourcerecordid><originalsourceid>FETCH-LOGICAL-a450t-8f7e5a0cbccda781a7905c564e1e6156e39f234bd17357303cfc70e5aa97b71c3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhS0EotvCkSvyBcElrZ3YcXKsqkKRqlaq4Bw5zkRr5NjGk7Tdf8TPxEsWekCC01h63zzP0yPkDWennLXV2XaZEsSzAVJZq2dkw0XNirKS7DnZsLJuCs5rfkSOEb8xlp9N_ZIclbwRrGVyQ35cPsYEiDZ4GkZ6b3HUs_V0urs5p3lGSDZuIWlHexfCQKfgg1-MA52oAeeQWqQ-zNSElMDpGQb6YOft31ZhAj9nHz3YGBDobBEX2CsPe2ndisHtzA5na2i412lHceeHlPVX5MWoHcLrwzwhXz9efrm4Kq5vP32-OL8utJBsLppRgdTM9MYMWjVcqxzTyFoAh5rLGqp2LCvRD1xVUlWsMqNRLK_oVvWKm-qEvF99YwrfF8C5myzug2oPYcGuaVmtZMVkJj_8k9zXI0QpSpXRYkVNCogJxi4mO-V4GfrFdWuN3Vpj5t8erJd-guEP_bu3DLw7ABqNdmPS3lh84oQUTMrm6cawxP_8-RNPYrmD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1093442427</pqid></control><display><type>article</type><title>Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Seow, Kok-Min ; Hwang, Jiann-Loung ; Wang, Peng-Hui ; Ho, Low-Tone ; Juan, Chi-Chang</creator><creatorcontrib>Seow, Kok-Min ; Hwang, Jiann-Loung ; Wang, Peng-Hui ; Ho, Low-Tone ; Juan, Chi-Chang</creatorcontrib><description>BACKGROUND
Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysis of gene expression. The aims of the study were to compare the expression of visfatin in PBMCs with that in omental adipose tissue in women with polycystic ovary syndrome (PCOS).
METHODS
Visfatin mRNA was measured in omental adipose tissue and PBMCs from 10 women with PCOS and 10 healthy controls, matched for BMI and age, using the real-time polymerase chain reaction (PCR).
RESULTS
The expression of visfatin mRNA in both omental adipose tissue and PBMCs from the women with PCOS was significantly higher (P= 0.01 and P= 0.05, respectively) than that in the controls. This finding indicated that mononuclear cells are a potential source of visfatin in women with PCOS. However, only the expression of visfatin mRNA in adipose tissue, not that in PBMCs, showed a significant positive correlation with insulin levels 2h after glucose loading (P= 0.044, r2 = 0.45), and with homeostasis model assessment–insulin resistance (HOMAIR; P= 0.035, r2 = 0.47). In addition, the expression of visfatin mRNA in PBMCs did not correlate with the expression of visfatin mRNA in omental adipose tissue.
CONCLUSIONS
PCOS is associated with increased visfatin mRNA concentrations in PBMCs and in omental adipose tissue. However, only visfatin mRNA concentration in omental adipose tissue is closely correlated with BMI and insulin resistance.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/der267</identifier><identifier>PMID: 21840905</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adipokines - biosynthesis ; Adipose tissue ; Adipose Tissue - cytology ; Adipose Tissue - pathology ; Adult ; Biological and medical sciences ; Female ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Glucose ; Gynecology. Andrology. Obstetrics ; Homeostasis ; Humans ; Insulin ; Insulin Resistance ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - cytology ; Medical sciences ; Nicotinamide Phosphoribosyltransferase - biosynthesis ; Obesity - complications ; Omentum - pathology ; Peripheral blood mononuclear cells ; polycystic ovary syndrome ; Polycystic Ovary Syndrome - metabolism ; Polycystic Ovary Syndrome - pathology ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction - methods ; RNA, Messenger - metabolism</subject><ispartof>Human reproduction (Oxford), 2011-10, Vol.26 (10), p.2869-2873</ispartof><rights>The Author 2011. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a450t-8f7e5a0cbccda781a7905c564e1e6156e39f234bd17357303cfc70e5aa97b71c3</citedby><cites>FETCH-LOGICAL-a450t-8f7e5a0cbccda781a7905c564e1e6156e39f234bd17357303cfc70e5aa97b71c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24540558$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21840905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seow, Kok-Min</creatorcontrib><creatorcontrib>Hwang, Jiann-Loung</creatorcontrib><creatorcontrib>Wang, Peng-Hui</creatorcontrib><creatorcontrib>Ho, Low-Tone</creatorcontrib><creatorcontrib>Juan, Chi-Chang</creatorcontrib><title>Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>BACKGROUND
Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysis of gene expression. The aims of the study were to compare the expression of visfatin in PBMCs with that in omental adipose tissue in women with polycystic ovary syndrome (PCOS).
METHODS
Visfatin mRNA was measured in omental adipose tissue and PBMCs from 10 women with PCOS and 10 healthy controls, matched for BMI and age, using the real-time polymerase chain reaction (PCR).
RESULTS
The expression of visfatin mRNA in both omental adipose tissue and PBMCs from the women with PCOS was significantly higher (P= 0.01 and P= 0.05, respectively) than that in the controls. This finding indicated that mononuclear cells are a potential source of visfatin in women with PCOS. However, only the expression of visfatin mRNA in adipose tissue, not that in PBMCs, showed a significant positive correlation with insulin levels 2h after glucose loading (P= 0.044, r2 = 0.45), and with homeostasis model assessment–insulin resistance (HOMAIR; P= 0.035, r2 = 0.47). In addition, the expression of visfatin mRNA in PBMCs did not correlate with the expression of visfatin mRNA in omental adipose tissue.
CONCLUSIONS
PCOS is associated with increased visfatin mRNA concentrations in PBMCs and in omental adipose tissue. However, only visfatin mRNA concentration in omental adipose tissue is closely correlated with BMI and insulin resistance.</description><subject>Adipokines - biosynthesis</subject><subject>Adipose tissue</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - pathology</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation</subject><subject>Glucose</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin Resistance</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Medical sciences</subject><subject>Nicotinamide Phosphoribosyltransferase - biosynthesis</subject><subject>Obesity - complications</subject><subject>Omentum - pathology</subject><subject>Peripheral blood mononuclear cells</subject><subject>polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - metabolism</subject><subject>Polycystic Ovary Syndrome - pathology</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - metabolism</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvCkSvyBcElrZ3YcXKsqkKRqlaq4Bw5zkRr5NjGk7Tdf8TPxEsWekCC01h63zzP0yPkDWennLXV2XaZEsSzAVJZq2dkw0XNirKS7DnZsLJuCs5rfkSOEb8xlp9N_ZIclbwRrGVyQ35cPsYEiDZ4GkZ6b3HUs_V0urs5p3lGSDZuIWlHexfCQKfgg1-MA52oAeeQWqQ-zNSElMDpGQb6YOft31ZhAj9nHz3YGBDobBEX2CsPe2ndisHtzA5na2i412lHceeHlPVX5MWoHcLrwzwhXz9efrm4Kq5vP32-OL8utJBsLppRgdTM9MYMWjVcqxzTyFoAh5rLGqp2LCvRD1xVUlWsMqNRLK_oVvWKm-qEvF99YwrfF8C5myzug2oPYcGuaVmtZMVkJj_8k9zXI0QpSpXRYkVNCogJxi4mO-V4GfrFdWuN3Vpj5t8erJd-guEP_bu3DLw7ABqNdmPS3lh84oQUTMrm6cawxP_8-RNPYrmD</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Seow, Kok-Min</creator><creator>Hwang, Jiann-Loung</creator><creator>Wang, Peng-Hui</creator><creator>Ho, Low-Tone</creator><creator>Juan, Chi-Chang</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome</title><author>Seow, Kok-Min ; Hwang, Jiann-Loung ; Wang, Peng-Hui ; Ho, Low-Tone ; Juan, Chi-Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a450t-8f7e5a0cbccda781a7905c564e1e6156e39f234bd17357303cfc70e5aa97b71c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipokines - biosynthesis</topic><topic>Adipose tissue</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - pathology</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation</topic><topic>Glucose</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin Resistance</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Medical sciences</topic><topic>Nicotinamide Phosphoribosyltransferase - biosynthesis</topic><topic>Obesity - complications</topic><topic>Omentum - pathology</topic><topic>Peripheral blood mononuclear cells</topic><topic>polycystic ovary syndrome</topic><topic>Polycystic Ovary Syndrome - metabolism</topic><topic>Polycystic Ovary Syndrome - pathology</topic><topic>Polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seow, Kok-Min</creatorcontrib><creatorcontrib>Hwang, Jiann-Loung</creatorcontrib><creatorcontrib>Wang, Peng-Hui</creatorcontrib><creatorcontrib>Ho, Low-Tone</creatorcontrib><creatorcontrib>Juan, Chi-Chang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seow, Kok-Min</au><au>Hwang, Jiann-Loung</au><au>Wang, Peng-Hui</au><au>Ho, Low-Tone</au><au>Juan, Chi-Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>26</volume><issue>10</issue><spage>2869</spage><epage>2873</epage><pages>2869-2873</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND
Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysis of gene expression. The aims of the study were to compare the expression of visfatin in PBMCs with that in omental adipose tissue in women with polycystic ovary syndrome (PCOS).
METHODS
Visfatin mRNA was measured in omental adipose tissue and PBMCs from 10 women with PCOS and 10 healthy controls, matched for BMI and age, using the real-time polymerase chain reaction (PCR).
RESULTS
The expression of visfatin mRNA in both omental adipose tissue and PBMCs from the women with PCOS was significantly higher (P= 0.01 and P= 0.05, respectively) than that in the controls. This finding indicated that mononuclear cells are a potential source of visfatin in women with PCOS. However, only the expression of visfatin mRNA in adipose tissue, not that in PBMCs, showed a significant positive correlation with insulin levels 2h after glucose loading (P= 0.044, r2 = 0.45), and with homeostasis model assessment–insulin resistance (HOMAIR; P= 0.035, r2 = 0.47). In addition, the expression of visfatin mRNA in PBMCs did not correlate with the expression of visfatin mRNA in omental adipose tissue.
CONCLUSIONS
PCOS is associated with increased visfatin mRNA concentrations in PBMCs and in omental adipose tissue. However, only visfatin mRNA concentration in omental adipose tissue is closely correlated with BMI and insulin resistance.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>21840905</pmid><doi>10.1093/humrep/der267</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Human reproduction (Oxford), 2011-10, Vol.26 (10), p.2869-2873 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adipokines - biosynthesis Adipose tissue Adipose Tissue - cytology Adipose Tissue - pathology Adult Biological and medical sciences Female Gene expression Gene Expression Profiling - methods Gene Expression Regulation Glucose Gynecology. Andrology. Obstetrics Homeostasis Humans Insulin Insulin Resistance Leukocytes (mononuclear) Leukocytes, Mononuclear - cytology Medical sciences Nicotinamide Phosphoribosyltransferase - biosynthesis Obesity - complications Omentum - pathology Peripheral blood mononuclear cells polycystic ovary syndrome Polycystic Ovary Syndrome - metabolism Polycystic Ovary Syndrome - pathology Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods RNA, Messenger - metabolism |
| title | Expression of visfatin mRNA in peripheral blood mononuclear cells is not correlated with visfatin mRNA in omental adipose tissue in women with polycystic ovary syndrome |
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