A Boyden chamber-based method for characterization of astrocyte protrusion localized RNA and protein

The Boyden chamber assay has been developed for various cell migration and invasion protocols. One variant of the Boyden chamber assay is the pseudopodium isolation assay, which has been developed to identify RNA and proteins localized in pseudopodia cell protrusions. Astrocytes are the most abundan...

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Veröffentlicht in:	Glia 2011-11, Vol.59 (11), p.1782-1792
Hauptverfasser:	Thomsen, Rune, Lade Nielsen, Anders
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Ankyrin Ankyrin Repeat - genetics Astrocytes Astrocytes - metabolism Biochemical analysis Blotting, Western Boyden chamber Brain Cell migration Cell Movement Cell Surface Extensions - metabolism Central nervous system Cytosol - metabolism Cytosol - ultrastructure Data Interpretation, Statistical Diffusion Chambers, Culture Fluorescent Antibody Technique Genomes Image Processing, Computer-Assisted Inositol Mice mRNA Nerve Tissue Proteins - metabolism Neuronal-glial interactions Neurons Neurotransmitters NIH 3T3 Cells Phosphoric Monoester Hydrolases - biosynthesis Phosphoric Monoester Hydrolases - genetics Plakophilins - biosynthesis Plakophilins - genetics protein localization Pseudopodia Pseudopodia - metabolism rab GTP-Binding Proteins - biosynthesis rab GTP-Binding Proteins - genetics Real-Time Polymerase Chain Reaction RNA localization RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Messenger - metabolism Silver Staining Synaptic cleft
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