Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the m...

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Veröffentlicht in:Journal of Biomedical Optics 2011-02, Vol.16 (2), p.021102-021102
Hauptverfasser: Lei, Ming, Winterhalder, Martin, Selm, Romedi, Zumbusch, Andreas
Format: Artikel
Sprache:eng
Schlagworte:
Beads
Cars
Coherent scattering
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Illumination
Light
Lighting - instrumentation
Microscopy
Microscopy - instrumentation
Microscopy, Video - instrumentation
Raman scattering
Reproducibility of Results
Scattering, Radiation
Sensitivity and Specificity
Signal generation
Spectrum Analysis, Raman - instrumentation
Tomography, Optical Coherence - methods
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Zusammenfassung:A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of embryos are shown, and the capabilities and the limitations of the scheme are discussed.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.3533707