Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy
We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous...
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| Veröffentlicht in: | Journal of Biomedical Optics 2010-05, Vol.15 (3), p.030515-030515 |
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| container_title | Journal of Biomedical Optics |
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| creator | Zhang, Leilei Xu, Jeff S Sanders, Virgil E Letson, Alan D Roberts, Cynthia J Xu, Ronald X |
| description | We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD. |
| doi_str_mv | 10.1117/1.3457669 |
| format | Article |
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Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.3457669</identifier><identifier>PMID: 20614998</identifier><identifier>CODEN: JBOPFO</identifier><language>eng</language><publisher>United States</publisher><subject>Angiogenesis Inhibitors - administration & dosage ; Angiogenesis Inhibitors - chemistry ; Antibodies, Monoclonal - administration & dosage ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal, Humanized ; Bevacizumab ; Conjugation ; Diagnostic Imaging - methods ; Drug Delivery Systems - methods ; Encapsulation ; Enzyme-Linked Immunosorbent Assay ; Exudation ; Growth factors ; Humans ; Imaging ; Logistic Models ; Macular Degeneration - drug therapy ; Microbubbles ; Microorganisms ; Microscopy, Electron, Scanning ; Therapy ; Ultrasound ; Vascular Endothelial Growth Factor A - antagonists & inhibitors</subject><ispartof>Journal of Biomedical Optics, 2010-05, Vol.15 (3), p.030515-030515</ispartof><rights>2011 COPYRIGHT SPIE--The International Society for Optical Engineering. 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Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD.</description><subject>Angiogenesis Inhibitors - administration & dosage</subject><subject>Angiogenesis Inhibitors - chemistry</subject><subject>Antibodies, Monoclonal - administration & dosage</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal, Humanized</subject><subject>Bevacizumab</subject><subject>Conjugation</subject><subject>Diagnostic Imaging - methods</subject><subject>Drug Delivery Systems - methods</subject><subject>Encapsulation</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Exudation</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Imaging</subject><subject>Logistic Models</subject><subject>Macular Degeneration - drug therapy</subject><subject>Microbubbles</subject><subject>Microorganisms</subject><subject>Microscopy, Electron, Scanning</subject><subject>Therapy</subject><subject>Ultrasound</subject><subject>Vascular Endothelial Growth Factor A - antagonists & inhibitors</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLAzEQgIMoVqsH_4DsTTxszWzeRy3WB5V60JuwZHezbWRfJrtK_70prb1KYDJMvpmBLwhdAJ4AgLiBCaFMcK4O0AkwjuMkkXAYcixJTDiXI3Tq_SfGWHLFj9EowRyoUvIEfbwMVW_Locl72za6imqbuzYbsqwyPipbF9laL028HGxhikg3vf3WPh8q7SLTFG2_MpUNbUvX_vSrqNR5H3pC1elufYaOSl15c767x-h9dv82fYzni4en6e08zomifQxlooQhmHIAlmspSFYwUXCegBBUSKG00gUQbgg1DErMNAuLWCl1prhJyBhdbed2rv0ajO_T2vrcVJVuTDv4VEpFsaJC_EsKQjgjQtFAXm_JoMN7Z8q0c0GFW6eA0431FNKd9cBe7qYOWW2KPfmnOQDJFvCdNfvn57vF62wRfgUD20RMwmG7HMgvQgWK9g</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Zhang, Leilei</creator><creator>Xu, Jeff S</creator><creator>Sanders, Virgil E</creator><creator>Letson, Alan D</creator><creator>Roberts, Cynthia J</creator><creator>Xu, Ronald X</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20100501</creationdate><title>Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy</title><author>Zhang, Leilei ; Xu, Jeff S ; Sanders, Virgil E ; Letson, Alan D ; Roberts, Cynthia J ; Xu, Ronald X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-1f297e3046115ca873bd57d66217747879a9ad136e34e51f05a5fac5f8ab96e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Angiogenesis Inhibitors - administration & dosage</topic><topic>Angiogenesis Inhibitors - chemistry</topic><topic>Antibodies, Monoclonal - administration & dosage</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal, Humanized</topic><topic>Bevacizumab</topic><topic>Conjugation</topic><topic>Diagnostic Imaging - methods</topic><topic>Drug Delivery Systems - methods</topic><topic>Encapsulation</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Exudation</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Imaging</topic><topic>Logistic Models</topic><topic>Macular Degeneration - drug therapy</topic><topic>Microbubbles</topic><topic>Microorganisms</topic><topic>Microscopy, Electron, Scanning</topic><topic>Therapy</topic><topic>Ultrasound</topic><topic>Vascular Endothelial Growth Factor A - antagonists & inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Leilei</creatorcontrib><creatorcontrib>Xu, Jeff S</creatorcontrib><creatorcontrib>Sanders, Virgil E</creatorcontrib><creatorcontrib>Letson, Alan D</creatorcontrib><creatorcontrib>Roberts, Cynthia J</creatorcontrib><creatorcontrib>Xu, Ronald X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of Biomedical Optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Leilei</au><au>Xu, Jeff S</au><au>Sanders, Virgil E</au><au>Letson, Alan D</au><au>Roberts, Cynthia J</au><au>Xu, Ronald X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy</atitle><jtitle>Journal of Biomedical Optics</jtitle><addtitle>J Biomed Opt</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>15</volume><issue>3</issue><spage>030515</spage><epage>030515</epage><pages>030515-030515</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><coden>JBOPFO</coden><abstract>We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD.</abstract><cop>United States</cop><pmid>20614998</pmid><doi>10.1117/1.3457669</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Angiogenesis Inhibitors - administration & dosage Angiogenesis Inhibitors - chemistry Antibodies, Monoclonal - administration & dosage Antibodies, Monoclonal - chemistry Antibodies, Monoclonal, Humanized Bevacizumab Conjugation Diagnostic Imaging - methods Drug Delivery Systems - methods Encapsulation Enzyme-Linked Immunosorbent Assay Exudation Growth factors Humans Imaging Logistic Models Macular Degeneration - drug therapy Microbubbles Microorganisms Microscopy, Electron, Scanning Therapy Ultrasound Vascular Endothelial Growth Factor A - antagonists & inhibitors |
| title | Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy |
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