Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction
A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-04, Vol.879 (13), p.927-932 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Deng, Biyang Ye, Li Yin, Huihui Liu, Yang Hu, Shangying Li, Baofeng |
| description | A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage, injection time, ultrasonic power and extraction time were investigated. Under optimal conditions, the linear concentration range for pseudolycorine was 0.002–2
μg/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N
=
3) was 0.46
ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata. |
| doi_str_mv | 10.1016/j.jchromb.2011.03.002 |
| format | Article |
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μg/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N
=
3) was 0.46
ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.03.002</identifier><identifier>PMID: 21429823</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amaryllidaceae Alkaloids - analysis ; Amaryllidaceae Alkaloids - isolation & purification ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Bulbs ; Capillarity ; Capillary electrophoresis ; Chemical Fractionation ; chromatography ; correlation ; detection limit ; Electrochemiluminescence ; Electrophoresis ; Electrophoresis, Capillary - methods ; Extraction ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Linear Models ; Luminescent Measurements ; Lycoris - chemistry ; Lycoris radiata ; Medical sciences ; On-line systems ; Online ; Pharmacology. Drug treatments ; Phenanthridines - analysis ; Phenanthridines - isolation & purification ; phosphates ; Plant Roots - chemistry ; Pseudolycorine ; Reproducibility of Results ; Sensitivity and Specificity ; Sonication - methods ; Ultrasonic extraction ; Ultrasonic testing ; ultrasonics</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-04, Vol.879 (13), p.927-932</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-ac450c88f8403b4dde1b1746de567ab013ea676871d26270246b18099e73149c3</citedby><cites>FETCH-LOGICAL-c450t-ac450c88f8403b4dde1b1746de567ab013ea676871d26270246b18099e73149c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2011.03.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24045824$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21429823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Biyang</creatorcontrib><creatorcontrib>Ye, Li</creatorcontrib><creatorcontrib>Yin, Huihui</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Hu, Shangying</creatorcontrib><creatorcontrib>Li, Baofeng</creatorcontrib><title>Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage, injection time, ultrasonic power and extraction time were investigated. Under optimal conditions, the linear concentration range for pseudolycorine was 0.002–2
μg/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N
=
3) was 0.46
ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata.</description><subject>Amaryllidaceae Alkaloids - analysis</subject><subject>Amaryllidaceae Alkaloids - isolation & purification</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bulbs</subject><subject>Capillarity</subject><subject>Capillary electrophoresis</subject><subject>Chemical Fractionation</subject><subject>chromatography</subject><subject>correlation</subject><subject>detection limit</subject><subject>Electrochemiluminescence</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Extraction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Linear Models</subject><subject>Luminescent Measurements</subject><subject>Lycoris - chemistry</subject><subject>Lycoris radiata</subject><subject>Medical sciences</subject><subject>On-line systems</subject><subject>Online</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenanthridines - analysis</subject><subject>Phenanthridines - isolation & purification</subject><subject>phosphates</subject><subject>Plant Roots - chemistry</subject><subject>Pseudolycorine</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sonication - methods</subject><subject>Ultrasonic extraction</subject><subject>Ultrasonic testing</subject><subject>ultrasonics</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRSMEYh7wCYA3iFVC-RHbWSE0MIA0EgsYiZ3lOJWJW0nc2AnQP8U34qgbWM6qLPvUreu6RfGMQkWByte7aueGGKa2YkBpBbwCYA-Kc6oVL7mS3x7mc62gBMbZWXGR0g6AKlD8cXHGqGCNZvy8-P0OF4yTn-3iw0xCT_YJ1y6MBxein5H4mSwDknYd2-31eJ9ItJ23iyXtgTi79-No44HgiG6JYT-EiClDLtvLGh356ZeBhHncBE-QG3Dy45onY3I4OyRr8vMdWccl2hRm70qbssiS2_FXvnObwSfFo96OCZ-e6mVxe_3-69XH8ubzh09Xb29KJ2pYSrsVp3WvBfBWdB3SliohO6ylsi1QjlYqqRXtmGQKmJAt1dA0qDgVjeOXxauj7j6G7yumxUw-28zfnDGsyWjdCKACmvtJSWutmaKZrI-kiyGliL3ZRz_lvRkKZsvU7MwpU7NlaoCbnGnue36asLYTdv-6_oaYgZcnwCZnxz7a2fn0nxMgas1E5l4cud4GY-9yjOb2S54kAUDWTMlMvDkSmHf7w2M0yfktnM7HnJrpgr_H7B_r5dBY</recordid><startdate>20110415</startdate><enddate>20110415</enddate><creator>Deng, Biyang</creator><creator>Ye, Li</creator><creator>Yin, Huihui</creator><creator>Liu, Yang</creator><creator>Hu, Shangying</creator><creator>Li, Baofeng</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20110415</creationdate><title>Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction</title><author>Deng, Biyang ; Ye, Li ; Yin, Huihui ; Liu, Yang ; Hu, Shangying ; Li, Baofeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-ac450c88f8403b4dde1b1746de567ab013ea676871d26270246b18099e73149c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amaryllidaceae Alkaloids - analysis</topic><topic>Amaryllidaceae Alkaloids - isolation & purification</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Bulbs</topic><topic>Capillarity</topic><topic>Capillary electrophoresis</topic><topic>Chemical Fractionation</topic><topic>chromatography</topic><topic>correlation</topic><topic>detection limit</topic><topic>Electrochemiluminescence</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Extraction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Linear Models</topic><topic>Luminescent Measurements</topic><topic>Lycoris - chemistry</topic><topic>Lycoris radiata</topic><topic>Medical sciences</topic><topic>On-line systems</topic><topic>Online</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenanthridines - analysis</topic><topic>Phenanthridines - isolation & purification</topic><topic>phosphates</topic><topic>Plant Roots - chemistry</topic><topic>Pseudolycorine</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sonication - methods</topic><topic>Ultrasonic extraction</topic><topic>Ultrasonic testing</topic><topic>ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Biyang</creatorcontrib><creatorcontrib>Ye, Li</creatorcontrib><creatorcontrib>Yin, Huihui</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Hu, Shangying</creatorcontrib><creatorcontrib>Li, Baofeng</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Biyang</au><au>Ye, Li</au><au>Yin, Huihui</au><au>Liu, Yang</au><au>Hu, Shangying</au><au>Li, Baofeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-04-15</date><risdate>2011</risdate><volume>879</volume><issue>13</issue><spage>927</spage><epage>932</epage><pages>927-932</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage, injection time, ultrasonic power and extraction time were investigated. Under optimal conditions, the linear concentration range for pseudolycorine was 0.002–2
μg/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N
=
3) was 0.46
ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21429823</pmid><doi>10.1016/j.jchromb.2011.03.002</doi><tpages>6</tpages></addata></record> |
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| subjects | Amaryllidaceae Alkaloids - analysis Amaryllidaceae Alkaloids - isolation & purification Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Bulbs Capillarity Capillary electrophoresis Chemical Fractionation chromatography correlation detection limit Electrochemiluminescence Electrophoresis Electrophoresis, Capillary - methods Extraction Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Linear Models Luminescent Measurements Lycoris - chemistry Lycoris radiata Medical sciences On-line systems Online Pharmacology. Drug treatments Phenanthridines - analysis Phenanthridines - isolation & purification phosphates Plant Roots - chemistry Pseudolycorine Reproducibility of Results Sensitivity and Specificity Sonication - methods Ultrasonic extraction Ultrasonic testing ultrasonics |
| title | Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction |
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