In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione
The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p
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| Veröffentlicht in: | Reproduction in domestic animals 2011-10, Vol.46 (5), p.874-881 |
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| container_title | Reproduction in domestic animals |
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| creator | Silva, SV Soares, AT Batista, AM Almeida, FC Nunes, JF Peixoto, CA Guerra, MMP |
| description | The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p |
| doi_str_mv | 10.1111/j.1439-0531.2011.01758.x |
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Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mm group had lesser TM and better STR than the other groups and the GSH 5 and 7 mm groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mm (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mm GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mm preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg‐yolk extender offers protection to the membranes of sperm cells after thawing.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2011.01758.x</identifier><identifier>PMID: 21332830</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>acrosome ; Animal reproduction ; Animals ; Biological and medical sciences ; Cell Membrane - drug effects ; Cytoprotection ; Enzymes ; Female ; freezing ; Fundamental and applied biological sciences. Psychology ; glutathione ; Glutathione - pharmacology ; Insemination, Artificial - veterinary ; Male ; Mammalian reproduction. General aspects ; Membrane Potential, Mitochondrial ; mitochondria ; plasma membrane ; rams ; semen ; Semen Preservation - veterinary ; Sheep ; Sheep - physiology ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - physiology ; superoxide dismutase ; Superoxide Dismutase - pharmacology ; thawing ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2011-10, Vol.46 (5), p.874-881</ispartof><rights>2011 Blackwell Verlag GmbH</rights><rights>2015 INIST-CNRS</rights><rights>2011 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5118-e504d9f28628a8b29c9005af73b95cb4d00781d2e6737b258fdbc1b38cca15a93</citedby><cites>FETCH-LOGICAL-c5118-e504d9f28628a8b29c9005af73b95cb4d00781d2e6737b258fdbc1b38cca15a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2011.01758.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2011.01758.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24516368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21332830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, SV</creatorcontrib><creatorcontrib>Soares, AT</creatorcontrib><creatorcontrib>Batista, AM</creatorcontrib><creatorcontrib>Almeida, FC</creatorcontrib><creatorcontrib>Nunes, JF</creatorcontrib><creatorcontrib>Peixoto, CA</creatorcontrib><creatorcontrib>Guerra, MMP</creatorcontrib><title>In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mm group had lesser TM and better STR than the other groups and the GSH 5 and 7 mm groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mm (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mm GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mm preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg‐yolk extender offers protection to the membranes of sperm cells after thawing.</description><subject>acrosome</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - drug effects</subject><subject>Cytoprotection</subject><subject>Enzymes</subject><subject>Female</subject><subject>freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glutathione</subject><subject>Glutathione - pharmacology</subject><subject>Insemination, Artificial - veterinary</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>Membrane Potential, Mitochondrial</subject><subject>mitochondria</subject><subject>plasma membrane</subject><subject>rams</subject><subject>semen</subject><subject>Semen Preservation - veterinary</subject><subject>Sheep</subject><subject>Sheep - physiology</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>superoxide dismutase</subject><subject>Superoxide Dismutase - pharmacology</subject><subject>thawing</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9v0zAUxyMEYmXwL4CFhDgl-EecOAcO09aVSROIthsSF8txnM5dEgc72VpOXLnxN_KX4DSlSBwQvtjv-fN9fnpfBwFAMEJ-vVlHKCZZCClBEYYIRRCllEWbB8HkcPEwmMCMJGGSJuwoeOLcGkJEWZo-Do4wIgQzAifB94sGXOvOGiCaAuyCOwOmd6LqRadNA0wJ5qIGi1bZGpxb81U1QDdgabUD09Xq57cfW1Pd7tSLvm0rVaumUwW4193NkFHWbHShwJl2dd8Jp3boXBW99NSs8rnuxj-kngaPSlE59Wy_HwdX59Pl6bvw8sPs4vTkMpQUIRYqCuMiKzFLMBMsx5nMIKSiTEmeUZnHBYQpQwVWSUrSHFNWFrlEOWFSCkRFRo6D12Pd1povvXIdr7WTqqpEo0zvOGMZShnB0JMv_yLXpreNb85DDMWJn6-H2AhJa5yzquSt1bWwW44gH8ziaz54wgdP-GAW35nFN176fF-_z2tVHIS_3fHAqz0gnBRVaUUjtfvDxRQlJGGeezty97pS2_9ugM_PToaT14ejXrtObQ56YW_5MEXKP72f8evPH_1UkiVfev7FyJfCcLHyX4FfLXzlGEIYkwTjfxKYEobJLzFR0cE</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Silva, SV</creator><creator>Soares, AT</creator><creator>Batista, AM</creator><creator>Almeida, FC</creator><creator>Nunes, JF</creator><creator>Peixoto, CA</creator><creator>Guerra, MMP</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione</title><author>Silva, SV ; Soares, AT ; Batista, AM ; Almeida, FC ; Nunes, JF ; Peixoto, CA ; Guerra, MMP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5118-e504d9f28628a8b29c9005af73b95cb4d00781d2e6737b258fdbc1b38cca15a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acrosome</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - drug effects</topic><topic>Cytoprotection</topic><topic>Enzymes</topic><topic>Female</topic><topic>freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glutathione</topic><topic>Glutathione - pharmacology</topic><topic>Insemination, Artificial - veterinary</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>Membrane Potential, Mitochondrial</topic><topic>mitochondria</topic><topic>plasma membrane</topic><topic>rams</topic><topic>semen</topic><topic>Semen Preservation - veterinary</topic><topic>Sheep</topic><topic>Sheep - physiology</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>superoxide dismutase</topic><topic>Superoxide Dismutase - pharmacology</topic><topic>thawing</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, SV</creatorcontrib><creatorcontrib>Soares, AT</creatorcontrib><creatorcontrib>Batista, AM</creatorcontrib><creatorcontrib>Almeida, FC</creatorcontrib><creatorcontrib>Nunes, JF</creatorcontrib><creatorcontrib>Peixoto, CA</creatorcontrib><creatorcontrib>Guerra, MMP</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, SV</au><au>Soares, AT</au><au>Batista, AM</au><au>Almeida, FC</au><au>Nunes, JF</au><au>Peixoto, CA</au><au>Guerra, MMP</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2011-10</date><risdate>2011</risdate><volume>46</volume><issue>5</issue><spage>874</spage><epage>881</epage><pages>874-881</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mm group had lesser TM and better STR than the other groups and the GSH 5 and 7 mm groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mm (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mm GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mm preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg‐yolk extender offers protection to the membranes of sperm cells after thawing.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21332830</pmid><doi>10.1111/j.1439-0531.2011.01758.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2011-10, Vol.46 (5), p.874-881 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | acrosome Animal reproduction Animals Biological and medical sciences Cell Membrane - drug effects Cytoprotection Enzymes Female freezing Fundamental and applied biological sciences. Psychology glutathione Glutathione - pharmacology Insemination, Artificial - veterinary Male Mammalian reproduction. General aspects Membrane Potential, Mitochondrial mitochondria plasma membrane rams semen Semen Preservation - veterinary Sheep Sheep - physiology Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - physiology superoxide dismutase Superoxide Dismutase - pharmacology thawing Vertebrates: reproduction |
| title | In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione |
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