Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep
The paraffin-embedded tissue (PET) blot was modified for use as a tool to differentiate between classical scrapie and experimental bovine spongiform encephalopathy (BSE) in sheep. Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2011-05, Vol.23 (3), p.492-498 |
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| creator | Webb, Paul R Denyer, Margaret Gough, Julie Spiropoulos, John Simmons, Marion M Spencer, Yvonne I |
| description | The paraffin-embedded tissue (PET) blot was modified for use as a tool to differentiate between classical scrapie and experimental bovine spongiform encephalopathy (BSE) in sheep. Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method such that it can be used as a tool to differentiate between BSE and scrapie in the same way that differential immunohistochemistry (IHC) has been used previously. The differential PET blot successfully differentiated between all of the scrapie and ovine BSE cases. Differentiation was permitted more easily with PET blot than by differential IHC, with accurate observations possible at the macroscopic level. At the microscopic level, sensitivity was such that discrimination by the differential PET blot could be made with more confidence than with differential IHC in cases where the immunohistochemical differences were subtle. The differential PET blot makes use of harsh epitope demasking conditions, and, because of the differences in the way prion protein is processed in different prion diseases, it can serve as a new, highly sensitive method to discriminate between classical scrapie and experimental BSE in sheep. |
| doi_str_mv | 10.1177/1040638711403399 |
| format | Article |
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Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method such that it can be used as a tool to differentiate between BSE and scrapie in the same way that differential immunohistochemistry (IHC) has been used previously. The differential PET blot successfully differentiated between all of the scrapie and ovine BSE cases. Differentiation was permitted more easily with PET blot than by differential IHC, with accurate observations possible at the macroscopic level. At the microscopic level, sensitivity was such that discrimination by the differential PET blot could be made with more confidence than with differential IHC in cases where the immunohistochemical differences were subtle. The differential PET blot makes use of harsh epitope demasking conditions, and, because of the differences in the way prion protein is processed in different prion diseases, it can serve as a new, highly sensitive method to discriminate between classical scrapie and experimental BSE in sheep.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/1040638711403399</identifier><identifier>PMID: 21908277</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; bovine spongiform encephalopathy ; Brain - pathology ; Cattle ; Encephalopathy, Bovine Spongiform - diagnosis ; Encephalopathy, Bovine Spongiform - pathology ; Immunoblotting - veterinary ; immunohistochemistry ; Paraffin Embedding - veterinary ; scrapie ; Scrapie - diagnosis ; Sensitivity and Specificity ; Sheep ; Sheep Diseases - diagnosis ; Sheep Diseases - pathology</subject><ispartof>Journal of veterinary diagnostic investigation, 2011-05, Vol.23 (3), p.492-498</ispartof><rights>2011 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-99d96829e62863fc4493904c26262dee5ef80f42e15a0c9c28f5e027f061eca3</citedby><cites>FETCH-LOGICAL-c468t-99d96829e62863fc4493904c26262dee5ef80f42e15a0c9c28f5e027f061eca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1040638711403399$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1040638711403399$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>315,782,786,21826,27931,27932,43628,43629</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21908277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Webb, Paul R</creatorcontrib><creatorcontrib>Denyer, Margaret</creatorcontrib><creatorcontrib>Gough, Julie</creatorcontrib><creatorcontrib>Spiropoulos, John</creatorcontrib><creatorcontrib>Simmons, Marion M</creatorcontrib><creatorcontrib>Spencer, Yvonne I</creatorcontrib><title>Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>The paraffin-embedded tissue (PET) blot was modified for use as a tool to differentiate between classical scrapie and experimental bovine spongiform encephalopathy (BSE) in sheep. Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method such that it can be used as a tool to differentiate between BSE and scrapie in the same way that differential immunohistochemistry (IHC) has been used previously. The differential PET blot successfully differentiated between all of the scrapie and ovine BSE cases. Differentiation was permitted more easily with PET blot than by differential IHC, with accurate observations possible at the macroscopic level. At the microscopic level, sensitivity was such that discrimination by the differential PET blot could be made with more confidence than with differential IHC in cases where the immunohistochemical differences were subtle. The differential PET blot makes use of harsh epitope demasking conditions, and, because of the differences in the way prion protein is processed in different prion diseases, it can serve as a new, highly sensitive method to discriminate between classical scrapie and experimental BSE in sheep.</description><subject>Animals</subject><subject>bovine spongiform encephalopathy</subject><subject>Brain - pathology</subject><subject>Cattle</subject><subject>Encephalopathy, Bovine Spongiform - diagnosis</subject><subject>Encephalopathy, Bovine Spongiform - pathology</subject><subject>Immunoblotting - veterinary</subject><subject>immunohistochemistry</subject><subject>Paraffin Embedding - veterinary</subject><subject>scrapie</subject><subject>Scrapie - diagnosis</subject><subject>Sensitivity and Specificity</subject><subject>Sheep</subject><subject>Sheep Diseases - diagnosis</subject><subject>Sheep Diseases - pathology</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhS1ERX9gzwq8YxWwncSxl6gCilSJSi3raOKM73WV2CHjFPosfVlc3cKCBZqFRzrfHOn4MPZaivdSdt0HKRqha9NJ2Yi6tvYZO5G2qavG1vp52YtcPerH7JToVohWtZ18wY6VtMKorjthD1ewgvchVjgPOI448hyINuTDlDIH4sAJI4Uc7pDPmPdp5D6tfAzk1jCHCDmkyAfMPxEjdxMQBQcTLzIsATnEkeOvBQuMMRdhSHchIqclxV0oVjPH6HDZw5QWyPt7HiKnPeLykh15mAhfPb1n7Obzp5vzi-ry25ev5x8vK9dokytrR6uNsqiV0bV3TUlvReOULjMituiN8I1C2YJw1injWxSq80JLdFCfsXcH22VNPzak3M8lG04TREwb9cbY8tetaQspDqRbE9GKvl9KKljveyn6x0L6fwspJ2-ezLdhxvHvwZ8GClAdAIId9rdpW2PJ-j_DtwfeQ-phtwbqv18rUWQhOq3rtv4NVjufkg</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Webb, Paul R</creator><creator>Denyer, Margaret</creator><creator>Gough, Julie</creator><creator>Spiropoulos, John</creator><creator>Simmons, Marion M</creator><creator>Spencer, Yvonne I</creator><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110501</creationdate><title>Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep</title><author>Webb, Paul R ; Denyer, Margaret ; Gough, Julie ; Spiropoulos, John ; Simmons, Marion M ; Spencer, Yvonne I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-99d96829e62863fc4493904c26262dee5ef80f42e15a0c9c28f5e027f061eca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>bovine spongiform encephalopathy</topic><topic>Brain - pathology</topic><topic>Cattle</topic><topic>Encephalopathy, Bovine Spongiform - diagnosis</topic><topic>Encephalopathy, Bovine Spongiform - pathology</topic><topic>Immunoblotting - veterinary</topic><topic>immunohistochemistry</topic><topic>Paraffin Embedding - veterinary</topic><topic>scrapie</topic><topic>Scrapie - diagnosis</topic><topic>Sensitivity and Specificity</topic><topic>Sheep</topic><topic>Sheep Diseases - diagnosis</topic><topic>Sheep Diseases - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Webb, Paul R</creatorcontrib><creatorcontrib>Denyer, Margaret</creatorcontrib><creatorcontrib>Gough, Julie</creatorcontrib><creatorcontrib>Spiropoulos, John</creatorcontrib><creatorcontrib>Simmons, Marion M</creatorcontrib><creatorcontrib>Spencer, Yvonne I</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Webb, Paul R</au><au>Denyer, Margaret</au><au>Gough, Julie</au><au>Spiropoulos, John</au><au>Simmons, Marion M</au><au>Spencer, Yvonne I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>23</volume><issue>3</issue><spage>492</spage><epage>498</epage><pages>492-498</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>The paraffin-embedded tissue (PET) blot was modified for use as a tool to differentiate between classical scrapie and experimental bovine spongiform encephalopathy (BSE) in sheep. Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method such that it can be used as a tool to differentiate between BSE and scrapie in the same way that differential immunohistochemistry (IHC) has been used previously. The differential PET blot successfully differentiated between all of the scrapie and ovine BSE cases. Differentiation was permitted more easily with PET blot than by differential IHC, with accurate observations possible at the macroscopic level. At the microscopic level, sensitivity was such that discrimination by the differential PET blot could be made with more confidence than with differential IHC in cases where the immunohistochemical differences were subtle. The differential PET blot makes use of harsh epitope demasking conditions, and, because of the differences in the way prion protein is processed in different prion diseases, it can serve as a new, highly sensitive method to discriminate between classical scrapie and experimental BSE in sheep.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>21908277</pmid><doi>10.1177/1040638711403399</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of veterinary diagnostic investigation, 2011-05, Vol.23 (3), p.492-498 |
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| language | eng |
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| source | Access via SAGE; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals bovine spongiform encephalopathy Brain - pathology Cattle Encephalopathy, Bovine Spongiform - diagnosis Encephalopathy, Bovine Spongiform - pathology Immunoblotting - veterinary immunohistochemistry Paraffin Embedding - veterinary scrapie Scrapie - diagnosis Sensitivity and Specificity Sheep Sheep Diseases - diagnosis Sheep Diseases - pathology |
| title | Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep |
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