Extended periods of neural induction and propagation of embryonic stem cell-derived neural progenitors with EGF and FGF2 enhances Lmx1a expression and neurogenic potential

► Short neural induction (NI) restricted the lineage potential of neural stem cells grown in FGF/EGF. ► Regardless of the differentiation protocol, four and seven day NI cultures gave rise to few neurons. ► Ten day NI enabled the robust generation of tyrosine and tryptophan hydroxylase positive neur...

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Veröffentlicht in:	Neurochemistry international 2011-09, Vol.59 (3), p.394-403
Hauptverfasser:	Zeng, Wendy R., Fabb, Stewart R.A., Haynes, John M., Pouton, Colin W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Differentiation Cell Lineage Cells, Cultured Culture Media Dopaminergic Embryonic stem cells Embryonic Stem Cells - cytology Epidermal Growth Factor - metabolism Fibroblast Growth Factor 2 - metabolism Flow Cytometry Fluorescent Dyes Gene Expression Regulation Immunohistochemistry In vitro differentiation LIM-Homeodomain Proteins - genetics Mice Multipotency Neural progenitors Neural stem cells Neurons - cytology Transcription Factors - genetics
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