Effects of cultural medium and conditions on the proliferation and hypoglycemic activity of Saccharomyces pastorianus no. 54
A yeast strain of Saccharomyces pastorianus no. 54 with hypoglycemic activity was isolated from soils of a winery. The aims of this study were first to investigate the effects of the cultivation conditions on proliferation and hypoglycemic activity of this yeast using the assay model of the differen...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2011-08, Vol.112 (2), p.159-165 |
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| creator | Wu, Chien-Hui Lin, Hong-Ting Wu, Guan-James Wang, Sheng-Hong Tsai, Guo-Jane |
| description | A yeast strain of
Saccharomyces pastorianus no. 54 with hypoglycemic activity was isolated from soils of a winery. The aims of this study were first to investigate the effects of the cultivation conditions on proliferation and hypoglycemic activity of this yeast using the assay model of the differentiated 3T3-L1 adipocytes, and then, to confirm in vivo the hypoglycemic activity of cultured yeast by oral administration in streptozotocin (STZ)-induced diabetic mice. Among 7 diluted fruit juice samples the diluted strawberry juice (1.74
g/L reducing sugar content) was chosen as the basal medium. After investigation of the effects of addition of various substances, including 1% of 5 different sugars and glycerol, 0.1% of 6 nitrogen-containing substances, and 1
ppm of 7 growth factors, the diluted strawberry juice added with 1% glucose, 0.1% yeast extract and 1
ppm aspartic acid was optimized at 20°C with initial pH value of 6.0 for cultivating
S. pastorianus no. 54 in flask. The scale-up system of a 5-L fermentor was further established by using the same medium with initial pH 6.0 and being incubated at 20°C with an aeration rate of 1.2
vvm for 96
h. The hypoglycemic activity of yeast cells cultivated in fermentor was 3.11 times of that in flask. Oral administration of the cultured yeast at a dosage of 130
mg/kg body weight/day for 6
days could significantly reduce the plasma glucose content in STZ-induced diabetic mice and keep their body weights in the normal range. |
| doi_str_mv | 10.1016/j.jbiosc.2011.04.012 |
| format | Article |
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Saccharomyces pastorianus no. 54 with hypoglycemic activity was isolated from soils of a winery. The aims of this study were first to investigate the effects of the cultivation conditions on proliferation and hypoglycemic activity of this yeast using the assay model of the differentiated 3T3-L1 adipocytes, and then, to confirm in vivo the hypoglycemic activity of cultured yeast by oral administration in streptozotocin (STZ)-induced diabetic mice. Among 7 diluted fruit juice samples the diluted strawberry juice (1.74
g/L reducing sugar content) was chosen as the basal medium. After investigation of the effects of addition of various substances, including 1% of 5 different sugars and glycerol, 0.1% of 6 nitrogen-containing substances, and 1
ppm of 7 growth factors, the diluted strawberry juice added with 1% glucose, 0.1% yeast extract and 1
ppm aspartic acid was optimized at 20°C with initial pH value of 6.0 for cultivating
S. pastorianus no. 54 in flask. The scale-up system of a 5-L fermentor was further established by using the same medium with initial pH 6.0 and being incubated at 20°C with an aeration rate of 1.2
vvm for 96
h. The hypoglycemic activity of yeast cells cultivated in fermentor was 3.11 times of that in flask. Oral administration of the cultured yeast at a dosage of 130
mg/kg body weight/day for 6
days could significantly reduce the plasma glucose content in STZ-induced diabetic mice and keep their body weights in the normal range.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2011.04.012</identifier><identifier>PMID: 21601518</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>3T3-L1 Cells ; Adipocytes ; Adipocytes - metabolism ; Administration, Oral ; aeration ; Animals ; aspartic acid ; Biological and medical sciences ; Biotechnology ; blood glucose ; body weight ; Carbohydrate Metabolism ; Cells, Cultured ; Cultural conditions ; Culture Media ; Diabetes ; Diabetes Mellitus, Experimental - blood ; Female ; Fermentation ; fermenters ; Fragaria ; fruit juices ; Fundamental and applied biological sciences. Psychology ; glucose ; Glucose - metabolism ; glycemic effect ; glycerol ; Glycerol - metabolism ; growth factors ; Hydrogen-Ion Concentration ; Hypoglycemic activity ; Hypoglycemic Agents - metabolism ; Hypoglycemic Agents - pharmacology ; Mice ; Mice, Inbred BALB C ; oral administration ; reducing sugars ; Saccharomyces - growth & development ; Saccharomyces - metabolism ; Saccharomyces pastorianus ; soil ; strawberries ; streptozotocin ; STZ-induced diabetic mice ; sugar content ; Temperature ; yeast extract ; yeasts</subject><ispartof>Journal of bioscience and bioengineering, 2011-08, Vol.112 (2), p.159-165</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-f4f4f843f95f8521ff9ec1db414c6e09b9ccc7f821a56b4a53da79fa83b85db43</citedby><cites>FETCH-LOGICAL-c537t-f4f4f843f95f8521ff9ec1db414c6e09b9ccc7f821a56b4a53da79fa83b85db43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiosc.2011.04.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24500793$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21601518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Chien-Hui</creatorcontrib><creatorcontrib>Lin, Hong-Ting</creatorcontrib><creatorcontrib>Wu, Guan-James</creatorcontrib><creatorcontrib>Wang, Sheng-Hong</creatorcontrib><creatorcontrib>Tsai, Guo-Jane</creatorcontrib><title>Effects of cultural medium and conditions on the proliferation and hypoglycemic activity of Saccharomyces pastorianus no. 54</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>A yeast strain of
Saccharomyces pastorianus no. 54 with hypoglycemic activity was isolated from soils of a winery. The aims of this study were first to investigate the effects of the cultivation conditions on proliferation and hypoglycemic activity of this yeast using the assay model of the differentiated 3T3-L1 adipocytes, and then, to confirm in vivo the hypoglycemic activity of cultured yeast by oral administration in streptozotocin (STZ)-induced diabetic mice. Among 7 diluted fruit juice samples the diluted strawberry juice (1.74
g/L reducing sugar content) was chosen as the basal medium. After investigation of the effects of addition of various substances, including 1% of 5 different sugars and glycerol, 0.1% of 6 nitrogen-containing substances, and 1
ppm of 7 growth factors, the diluted strawberry juice added with 1% glucose, 0.1% yeast extract and 1
ppm aspartic acid was optimized at 20°C with initial pH value of 6.0 for cultivating
S. pastorianus no. 54 in flask. The scale-up system of a 5-L fermentor was further established by using the same medium with initial pH 6.0 and being incubated at 20°C with an aeration rate of 1.2
vvm for 96
h. The hypoglycemic activity of yeast cells cultivated in fermentor was 3.11 times of that in flask. Oral administration of the cultured yeast at a dosage of 130
mg/kg body weight/day for 6
days could significantly reduce the plasma glucose content in STZ-induced diabetic mice and keep their body weights in the normal range.</description><subject>3T3-L1 Cells</subject><subject>Adipocytes</subject><subject>Adipocytes - metabolism</subject><subject>Administration, Oral</subject><subject>aeration</subject><subject>Animals</subject><subject>aspartic acid</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>blood glucose</subject><subject>body weight</subject><subject>Carbohydrate Metabolism</subject><subject>Cells, Cultured</subject><subject>Cultural conditions</subject><subject>Culture Media</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Experimental - blood</subject><subject>Female</subject><subject>Fermentation</subject><subject>fermenters</subject><subject>Fragaria</subject><subject>fruit juices</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>glycemic effect</subject><subject>glycerol</subject><subject>Glycerol - metabolism</subject><subject>growth factors</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hypoglycemic activity</subject><subject>Hypoglycemic Agents - metabolism</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>oral administration</subject><subject>reducing sugars</subject><subject>Saccharomyces - growth & development</subject><subject>Saccharomyces - metabolism</subject><subject>Saccharomyces pastorianus</subject><subject>soil</subject><subject>strawberries</subject><subject>streptozotocin</subject><subject>STZ-induced diabetic mice</subject><subject>sugar content</subject><subject>Temperature</subject><subject>yeast extract</subject><subject>yeasts</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-L1TAQgIso7rr6H4jmIntqzbRJm14EWXZVWPCw7jlMp8m-PNrmmbQLD_zjTe1TbyI5JEy--cF8WfYaeAEc6vf7Yt85H6koOUDBRcGhfJKdQyWaXIgSnq5v1ebQlNVZ9iLGPefQ8AaeZ2cl1BwkqPPsx7W1hubIvGW0DPMScGCj6d0yMpx6Rn7q3ez8lIiJzTvDDsEPzpqAa_QXszse_MNwJDM6Ykize3TzcS14h0Q7DH5Mf5EdMM4-OJyWyCZfMCleZs8sDtG8Ot0X2f3N9berz_nt109frj7e5iSrZs6tSEeJyrbSKlmCta0h6DsBgmrD264losaqElDWnUBZ9di0FlXVKZmw6iK73Oqm2b8vJs56dJHMMOBk_BK1UgqgBGj_g0wLlCBVIsVGUvAxBmP1IbgRw1ED16sgvdebIL0K0lzoJCilvTk1WLq05j9Jv40k4N0JwEg42IATufiXE5Lzpq0S93bjLHqNDyEx93epU805L4Wo60R82AiTVvvoTNCRnJkoyQ1Jue69-_esPwE8hbuO</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Wu, Chien-Hui</creator><creator>Lin, Hong-Ting</creator><creator>Wu, Guan-James</creator><creator>Wang, Sheng-Hong</creator><creator>Tsai, Guo-Jane</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20110801</creationdate><title>Effects of cultural medium and conditions on the proliferation and hypoglycemic activity of Saccharomyces pastorianus no. 54</title><author>Wu, Chien-Hui ; Lin, Hong-Ting ; Wu, Guan-James ; Wang, Sheng-Hong ; Tsai, Guo-Jane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-f4f4f843f95f8521ff9ec1db414c6e09b9ccc7f821a56b4a53da79fa83b85db43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>3T3-L1 Cells</topic><topic>Adipocytes</topic><topic>Adipocytes - metabolism</topic><topic>Administration, Oral</topic><topic>aeration</topic><topic>Animals</topic><topic>aspartic acid</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>blood glucose</topic><topic>body weight</topic><topic>Carbohydrate Metabolism</topic><topic>Cells, Cultured</topic><topic>Cultural conditions</topic><topic>Culture Media</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Experimental - blood</topic><topic>Female</topic><topic>Fermentation</topic><topic>fermenters</topic><topic>Fragaria</topic><topic>fruit juices</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>glycemic effect</topic><topic>glycerol</topic><topic>Glycerol - metabolism</topic><topic>growth factors</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hypoglycemic activity</topic><topic>Hypoglycemic Agents - metabolism</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>oral administration</topic><topic>reducing sugars</topic><topic>Saccharomyces - growth & development</topic><topic>Saccharomyces - metabolism</topic><topic>Saccharomyces pastorianus</topic><topic>soil</topic><topic>strawberries</topic><topic>streptozotocin</topic><topic>STZ-induced diabetic mice</topic><topic>sugar content</topic><topic>Temperature</topic><topic>yeast extract</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chien-Hui</creatorcontrib><creatorcontrib>Lin, Hong-Ting</creatorcontrib><creatorcontrib>Wu, Guan-James</creatorcontrib><creatorcontrib>Wang, Sheng-Hong</creatorcontrib><creatorcontrib>Tsai, Guo-Jane</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chien-Hui</au><au>Lin, Hong-Ting</au><au>Wu, Guan-James</au><au>Wang, Sheng-Hong</au><au>Tsai, Guo-Jane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of cultural medium and conditions on the proliferation and hypoglycemic activity of Saccharomyces pastorianus no. 54</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>112</volume><issue>2</issue><spage>159</spage><epage>165</epage><pages>159-165</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>A yeast strain of
Saccharomyces pastorianus no. 54 with hypoglycemic activity was isolated from soils of a winery. The aims of this study were first to investigate the effects of the cultivation conditions on proliferation and hypoglycemic activity of this yeast using the assay model of the differentiated 3T3-L1 adipocytes, and then, to confirm in vivo the hypoglycemic activity of cultured yeast by oral administration in streptozotocin (STZ)-induced diabetic mice. Among 7 diluted fruit juice samples the diluted strawberry juice (1.74
g/L reducing sugar content) was chosen as the basal medium. After investigation of the effects of addition of various substances, including 1% of 5 different sugars and glycerol, 0.1% of 6 nitrogen-containing substances, and 1
ppm of 7 growth factors, the diluted strawberry juice added with 1% glucose, 0.1% yeast extract and 1
ppm aspartic acid was optimized at 20°C with initial pH value of 6.0 for cultivating
S. pastorianus no. 54 in flask. The scale-up system of a 5-L fermentor was further established by using the same medium with initial pH 6.0 and being incubated at 20°C with an aeration rate of 1.2
vvm for 96
h. The hypoglycemic activity of yeast cells cultivated in fermentor was 3.11 times of that in flask. Oral administration of the cultured yeast at a dosage of 130
mg/kg body weight/day for 6
days could significantly reduce the plasma glucose content in STZ-induced diabetic mice and keep their body weights in the normal range.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21601518</pmid><doi>10.1016/j.jbiosc.2011.04.012</doi><tpages>7</tpages></addata></record> |
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| subjects | 3T3-L1 Cells Adipocytes Adipocytes - metabolism Administration, Oral aeration Animals aspartic acid Biological and medical sciences Biotechnology blood glucose body weight Carbohydrate Metabolism Cells, Cultured Cultural conditions Culture Media Diabetes Diabetes Mellitus, Experimental - blood Female Fermentation fermenters Fragaria fruit juices Fundamental and applied biological sciences. Psychology glucose Glucose - metabolism glycemic effect glycerol Glycerol - metabolism growth factors Hydrogen-Ion Concentration Hypoglycemic activity Hypoglycemic Agents - metabolism Hypoglycemic Agents - pharmacology Mice Mice, Inbred BALB C oral administration reducing sugars Saccharomyces - growth & development Saccharomyces - metabolism Saccharomyces pastorianus soil strawberries streptozotocin STZ-induced diabetic mice sugar content Temperature yeast extract yeasts |
| title | Effects of cultural medium and conditions on the proliferation and hypoglycemic activity of Saccharomyces pastorianus no. 54 |
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