Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity
Precision cut tissue slices (PCTS) represent a suitable and convenient tool for pharmacological, toxicological and morphological studies. Cryopreservation would enable to overcome the shortage of liver tissue, in particular in settings using human liver tissue. We investigated the potential of cryop...
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Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2011-09, Vol.63 (6), p.575-580 |
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container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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creator | Kasper, Hans-Udo Konze, Elisabeth Kutinová Canová, Nikolina Dienes, Hans Peter Dries, Volker |
description | Precision cut tissue slices (PCTS) represent a suitable and convenient tool for pharmacological, toxicological and morphological studies. Cryopreservation would enable to overcome the shortage of liver tissue, in particular in settings using human liver tissue. We investigated the potential of cryopreservation of porcine PCTS as a morphological tool by rapid freezing with 10% and 30% dimethyl sulfoxide as cryopreservation agents and with or without medium using a Brendel/Vitron tissue slicer. Incubation after thawing was done in a static incubation system. Slices were cultured for 3
h, 6
h, 24
h and 48
h and assessed histologically and immunohistologically for proliferation (Ki67) and spontaneous as well as induced apoptotic activity (M30Cytodeath). Vitality was tested using the Tox-8 test. After cryopreservation, morphology of PCTS was well preserved up to 24
h. A reduction of vitality rate took place. Compared to non-frozen PCTS, the rate of spontaneous proliferation of Kupffer cells and apoptosis of hepatocytes were significantly reduced independent of the freezing conditions. The reactivity of PCTS to apoptotic stimuli was significantly reduced in tissue slices after cryopreservation. Apoptotic stimuli could not induce the same amount of cell deaths compared to non-frozen sections. Thus, cryopreservation of PCTS does interfere with pathomechanisms of apoptosis in PCTS. |
doi_str_mv | 10.1016/j.etp.2010.04.010 |
format | Article |
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h, 6
h, 24
h and 48
h and assessed histologically and immunohistologically for proliferation (Ki67) and spontaneous as well as induced apoptotic activity (M30Cytodeath). Vitality was tested using the Tox-8 test. After cryopreservation, morphology of PCTS was well preserved up to 24
h. A reduction of vitality rate took place. Compared to non-frozen PCTS, the rate of spontaneous proliferation of Kupffer cells and apoptosis of hepatocytes were significantly reduced independent of the freezing conditions. The reactivity of PCTS to apoptotic stimuli was significantly reduced in tissue slices after cryopreservation. Apoptotic stimuli could not induce the same amount of cell deaths compared to non-frozen sections. Thus, cryopreservation of PCTS does interfere with pathomechanisms of apoptosis in PCTS.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2010.04.010</identifier><identifier>PMID: 20494565</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Cell Proliferation ; Cryopreservation ; dimethyl sulfoxide ; freezing ; hepatocytes ; humans ; Investigative techniques, diagnostic techniques (general aspects) ; Kupffer cells ; Liver ; Liver - anatomy & histology ; Liver - pathology ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Precision cut tissue slices ; Swine ; thawing ; Tissue Culture Techniques - methods ; Tissue Preservation - methods</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2011-09, Vol.63 (6), p.575-580</ispartof><rights>2010 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-53ce131b4e4ae057164f621ff4112e8c8fd4f5f34c699622b505c1bd32124b783</citedby><cites>FETCH-LOGICAL-c504t-53ce131b4e4ae057164f621ff4112e8c8fd4f5f34c699622b505c1bd32124b783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.etp.2010.04.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24453960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20494565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasper, Hans-Udo</creatorcontrib><creatorcontrib>Konze, Elisabeth</creatorcontrib><creatorcontrib>Kutinová Canová, Nikolina</creatorcontrib><creatorcontrib>Dienes, Hans Peter</creatorcontrib><creatorcontrib>Dries, Volker</creatorcontrib><title>Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>Precision cut tissue slices (PCTS) represent a suitable and convenient tool for pharmacological, toxicological and morphological studies. Cryopreservation would enable to overcome the shortage of liver tissue, in particular in settings using human liver tissue. We investigated the potential of cryopreservation of porcine PCTS as a morphological tool by rapid freezing with 10% and 30% dimethyl sulfoxide as cryopreservation agents and with or without medium using a Brendel/Vitron tissue slicer. Incubation after thawing was done in a static incubation system. Slices were cultured for 3
h, 6
h, 24
h and 48
h and assessed histologically and immunohistologically for proliferation (Ki67) and spontaneous as well as induced apoptotic activity (M30Cytodeath). Vitality was tested using the Tox-8 test. After cryopreservation, morphology of PCTS was well preserved up to 24
h. A reduction of vitality rate took place. Compared to non-frozen PCTS, the rate of spontaneous proliferation of Kupffer cells and apoptosis of hepatocytes were significantly reduced independent of the freezing conditions. The reactivity of PCTS to apoptotic stimuli was significantly reduced in tissue slices after cryopreservation. Apoptotic stimuli could not induce the same amount of cell deaths compared to non-frozen sections. Thus, cryopreservation of PCTS does interfere with pathomechanisms of apoptosis in PCTS.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Cryopreservation</subject><subject>dimethyl sulfoxide</subject><subject>freezing</subject><subject>hepatocytes</subject><subject>humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kupffer cells</subject><subject>Liver</subject><subject>Liver - anatomy & histology</subject><subject>Liver - pathology</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Precision cut tissue slices</subject><subject>Swine</subject><subject>thawing</subject><subject>Tissue Culture Techniques - methods</subject><subject>Tissue Preservation - methods</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vEzEQxS0EomnhA3CBvSDgsMHjP5s1nFBUoFIlkNqeLa93HBxt1ovtjZRvj6OEcuP09KTfvBm9IeQV0CVQaD5ul5inJaPFU7Es8oQsoIG2BsH5U7KgStCaKcUvyGVKW0oZVRKekwtGhRKykQvSreMhTBETxr3JPoxVcFXx1qejsXOusk9pxioN3mKq3v9c3999-FTdjHtM2W8eh3YhTr_CEDaHyox9FdHY7Pc-H16QZ84MCV-e9Yo8fL2-X3-vb398u1l_ua2tpCLXklsEDp1AYZDKFTTCNQycEwAMW9u6XjjpuLCNUg1jnaTSQtdzBkx0q5ZfkXen3CmG33M5Tu98sjgMZsQwJ922LQCsBBQSTqSNIaWITk_R70w8aKD62Kze6tKsPjarqdBFyszrc_rc7bB_nPhbZQHengGTrBlcNGMp8R8nhOSqOQa9OXHOBG02sTAPd2WTpGWx5FQV4vOJwNLW3mPUyXocLfa-PCbrPvj_HPoH2ZqfWQ</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Kasper, Hans-Udo</creator><creator>Konze, Elisabeth</creator><creator>Kutinová Canová, Nikolina</creator><creator>Dienes, Hans Peter</creator><creator>Dries, Volker</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20110901</creationdate><title>Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity</title><author>Kasper, Hans-Udo ; Konze, Elisabeth ; Kutinová Canová, Nikolina ; Dienes, Hans Peter ; Dries, Volker</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-53ce131b4e4ae057164f621ff4112e8c8fd4f5f34c699622b505c1bd32124b783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Cryopreservation</topic><topic>dimethyl sulfoxide</topic><topic>freezing</topic><topic>hepatocytes</topic><topic>humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kupffer cells</topic><topic>Liver</topic><topic>Liver - anatomy & histology</topic><topic>Liver - pathology</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Precision cut tissue slices</topic><topic>Swine</topic><topic>thawing</topic><topic>Tissue Culture Techniques - methods</topic><topic>Tissue Preservation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasper, Hans-Udo</creatorcontrib><creatorcontrib>Konze, Elisabeth</creatorcontrib><creatorcontrib>Kutinová Canová, Nikolina</creatorcontrib><creatorcontrib>Dienes, Hans Peter</creatorcontrib><creatorcontrib>Dries, Volker</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasper, Hans-Udo</au><au>Konze, Elisabeth</au><au>Kutinová Canová, Nikolina</au><au>Dienes, Hans Peter</au><au>Dries, Volker</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity</atitle><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle><addtitle>Exp Toxicol Pathol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>63</volume><issue>6</issue><spage>575</spage><epage>580</epage><pages>575-580</pages><issn>0940-2993</issn><eissn>1618-1433</eissn><abstract>Precision cut tissue slices (PCTS) represent a suitable and convenient tool for pharmacological, toxicological and morphological studies. Cryopreservation would enable to overcome the shortage of liver tissue, in particular in settings using human liver tissue. We investigated the potential of cryopreservation of porcine PCTS as a morphological tool by rapid freezing with 10% and 30% dimethyl sulfoxide as cryopreservation agents and with or without medium using a Brendel/Vitron tissue slicer. Incubation after thawing was done in a static incubation system. Slices were cultured for 3
h, 6
h, 24
h and 48
h and assessed histologically and immunohistologically for proliferation (Ki67) and spontaneous as well as induced apoptotic activity (M30Cytodeath). Vitality was tested using the Tox-8 test. After cryopreservation, morphology of PCTS was well preserved up to 24
h. A reduction of vitality rate took place. Compared to non-frozen PCTS, the rate of spontaneous proliferation of Kupffer cells and apoptosis of hepatocytes were significantly reduced independent of the freezing conditions. The reactivity of PCTS to apoptotic stimuli was significantly reduced in tissue slices after cryopreservation. Apoptotic stimuli could not induce the same amount of cell deaths compared to non-frozen sections. Thus, cryopreservation of PCTS does interfere with pathomechanisms of apoptosis in PCTS.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>20494565</pmid><doi>10.1016/j.etp.2010.04.010</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Apoptosis Biological and medical sciences Cell Proliferation Cryopreservation dimethyl sulfoxide freezing hepatocytes humans Investigative techniques, diagnostic techniques (general aspects) Kupffer cells Liver Liver - anatomy & histology Liver - pathology Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Precision cut tissue slices Swine thawing Tissue Culture Techniques - methods Tissue Preservation - methods |
title | Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity |
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