Identification of gene disruptions for increased poly-3-hydroxybutyrate accumulation in Synechocystis PCC 6803

Inverse metabolic engineering (IME) is a combinatorial approach for identifying genotypes associated with a particular phenotype of interest. In this study, gene disruptions that increase the biosynthesis of poly‐3‐hydroxybutyrate (PHB) in the photosynthetic bacterium Synechocystis PCC6803 were iden...

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Veröffentlicht in:	Biotechnology progress 2009-09, Vol.25 (5), p.1236-1243
Hauptverfasser:	Tyo, Keith E. J., Jin, Yong-Su, Espinoza, Freddy A., Stephanopoulos, Gregory
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde Oxidoreductases - genetics Aldehyde Oxidoreductases - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biotechnology Cloning, Molecular - methods Combinatorial Chemistry Techniques - methods Culture Media - chemistry Culture Media - metabolism DNA Transposable Elements - genetics Flow Cytometry fluorescence-activated cell sorting (FACS) Fundamental and applied biological sciences. Psychology Gene Library Hydroxybutyrates - metabolism inverse metabolic engineering Mutation Plasmids - genetics Polyesters - metabolism polyhydroxyalkanoate Synechocystis Synechocystis - enzymology Synechocystis - genetics Synechocystis - metabolism Systems Biology - methods transposon mutagenesis
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