Optimization of C:N ratio and minimal initial carbon source for poly(3-hydroxybutyrate) production by Bacillus megaterium

BACKGROUND: The aim of this research was the optimization of poly(3-hydroxybutyrate)--P(3HB)--production in submerged cultures of Bacillus megaterium in a mineral medium using sucrose as carbon source and nitrogen as the limiting substrate. Small-scale experiments were carried out in shake flasks at...

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Veröffentlicht in:Journal of chemical technology and biotechnology (1986) 2009-12, Vol.84 (12), p.1756-1761
Hauptverfasser: Faccin, Débora Jung Luvizetto, Martins, Ivana, Cardozo, Nilo Sérgio Medeiros, Rech, Rosane, Ayub, Marco Antônio Záchia, Alves, Tito Lívio Moitinho, Gambetta, Rossano, Resende Secchi, Argimiro
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container_end_page 1761
container_issue 12
container_start_page 1756
container_title Journal of chemical technology and biotechnology (1986)
container_volume 84
creator Faccin, Débora Jung Luvizetto
Martins, Ivana
Cardozo, Nilo Sérgio Medeiros
Rech, Rosane
Ayub, Marco Antônio Záchia
Alves, Tito Lívio Moitinho
Gambetta, Rossano
Resende Secchi, Argimiro
description BACKGROUND: The aim of this research was the optimization of poly(3-hydroxybutyrate)--P(3HB)--production in submerged cultures of Bacillus megaterium in a mineral medium using sucrose as carbon source and nitrogen as the limiting substrate. Small-scale experiments were carried out in shake flasks at 30 °C and 160 rpm in order to evaluate the best initial sucrose concentration and carbon:nitrogen ratio to maximize biomass accumulation and biopolymer production. An objective function in terms of residual sucrose and P(3HB) production was proposed in order to optimize the amount of carbon source used and the production of P(3HB).RESULTS: High production of P(3HB) was obtained, with approximately 70% (CDW) accumulation in cells without nitrogen limitation and strongly correlated with the pH of the culture. Scaling-up the system to cultures in a bioreactor, with or without pH control, a reduction of P(3HB) accumulation (around 30% CDW) was observed when compared with shaker cultures, suggesting a possible role of oxygen limitation as a stress signaling for P(3HB) synthesis.CONCLUSIONS: Results of our experiments showed that Bacillus megaterium was able to produce P(3HB) at one of the highest production rates so far reported for this bacterium, making this microorganism very interesting for industrial applications. Comparisons of shaker and bench-scale bioreactor experiments show both the importance of pH and aeration strategies. It is likely that complex aeration strategies linked to cell metabolism will be necessary for further developments using this bacterium. Copyright
doi_str_mv 10.1002/jctb.2240
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Small-scale experiments were carried out in shake flasks at 30 °C and 160 rpm in order to evaluate the best initial sucrose concentration and carbon:nitrogen ratio to maximize biomass accumulation and biopolymer production. An objective function in terms of residual sucrose and P(3HB) production was proposed in order to optimize the amount of carbon source used and the production of P(3HB).RESULTS: High production of P(3HB) was obtained, with approximately 70% (CDW) accumulation in cells without nitrogen limitation and strongly correlated with the pH of the culture. Scaling-up the system to cultures in a bioreactor, with or without pH control, a reduction of P(3HB) accumulation (around 30% CDW) was observed when compared with shaker cultures, suggesting a possible role of oxygen limitation as a stress signaling for P(3HB) synthesis.CONCLUSIONS: Results of our experiments showed that Bacillus megaterium was able to produce P(3HB) at one of the highest production rates so far reported for this bacterium, making this microorganism very interesting for industrial applications. Comparisons of shaker and bench-scale bioreactor experiments show both the importance of pH and aeration strategies. It is likely that complex aeration strategies linked to cell metabolism will be necessary for further developments using this bacterium. Copyright</description><identifier>ISSN: 0268-2575</identifier><identifier>ISSN: 1097-4660</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.2240</identifier><identifier>CODEN: JCTBDC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>Applied sciences ; Bacillus megaterium ; Biological and medical sciences ; biopolymers ; Bioreactors ; Biotechnology ; Carbon ; Chemical engineering ; Culture ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Chem. Technol. Biotechnol</addtitle><description>BACKGROUND: The aim of this research was the optimization of poly(3-hydroxybutyrate)--P(3HB)--production in submerged cultures of Bacillus megaterium in a mineral medium using sucrose as carbon source and nitrogen as the limiting substrate. Small-scale experiments were carried out in shake flasks at 30 °C and 160 rpm in order to evaluate the best initial sucrose concentration and carbon:nitrogen ratio to maximize biomass accumulation and biopolymer production. An objective function in terms of residual sucrose and P(3HB) production was proposed in order to optimize the amount of carbon source used and the production of P(3HB).RESULTS: High production of P(3HB) was obtained, with approximately 70% (CDW) accumulation in cells without nitrogen limitation and strongly correlated with the pH of the culture. Scaling-up the system to cultures in a bioreactor, with or without pH control, a reduction of P(3HB) accumulation (around 30% CDW) was observed when compared with shaker cultures, suggesting a possible role of oxygen limitation as a stress signaling for P(3HB) synthesis.CONCLUSIONS: Results of our experiments showed that Bacillus megaterium was able to produce P(3HB) at one of the highest production rates so far reported for this bacterium, making this microorganism very interesting for industrial applications. Comparisons of shaker and bench-scale bioreactor experiments show both the importance of pH and aeration strategies. It is likely that complex aeration strategies linked to cell metabolism will be necessary for further developments using this bacterium. 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Chem. Technol. Biotechnol</addtitle><date>2009-12</date><risdate>2009</risdate><volume>84</volume><issue>12</issue><spage>1756</spage><epage>1761</epage><pages>1756-1761</pages><issn>0268-2575</issn><issn>1097-4660</issn><eissn>1097-4660</eissn><coden>JCTBDC</coden><abstract>BACKGROUND: The aim of this research was the optimization of poly(3-hydroxybutyrate)--P(3HB)--production in submerged cultures of Bacillus megaterium in a mineral medium using sucrose as carbon source and nitrogen as the limiting substrate. Small-scale experiments were carried out in shake flasks at 30 °C and 160 rpm in order to evaluate the best initial sucrose concentration and carbon:nitrogen ratio to maximize biomass accumulation and biopolymer production. An objective function in terms of residual sucrose and P(3HB) production was proposed in order to optimize the amount of carbon source used and the production of P(3HB).RESULTS: High production of P(3HB) was obtained, with approximately 70% (CDW) accumulation in cells without nitrogen limitation and strongly correlated with the pH of the culture. Scaling-up the system to cultures in a bioreactor, with or without pH control, a reduction of P(3HB) accumulation (around 30% CDW) was observed when compared with shaker cultures, suggesting a possible role of oxygen limitation as a stress signaling for P(3HB) synthesis.CONCLUSIONS: Results of our experiments showed that Bacillus megaterium was able to produce P(3HB) at one of the highest production rates so far reported for this bacterium, making this microorganism very interesting for industrial applications. Comparisons of shaker and bench-scale bioreactor experiments show both the importance of pH and aeration strategies. It is likely that complex aeration strategies linked to cell metabolism will be necessary for further developments using this bacterium. Copyright</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><doi>10.1002/jctb.2240</doi><tpages>6</tpages></addata></record>
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source Wiley Online Library Journals Frontfile Complete
subjects Applied sciences
Bacillus megaterium
Biological and medical sciences
biopolymers
Bioreactors
Biotechnology
Carbon
Chemical engineering
Culture
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
optimization
Others
poly(3-hydroxybutyrate)
Reactors
Shakers
Strategy
Sucrose
Various methods and equipments
title Optimization of C:N ratio and minimal initial carbon source for poly(3-hydroxybutyrate) production by Bacillus megaterium
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