A two-culture method for exposure of human brain organotypic slice cultures to replicating human immunodeficiency virus type 1

To evaluate the effect of HIV-1 virus on neural cells, we have developed a method to culture human fetal organotypic brain slices in the presence of live virus. Brain slices were placed on semipermeable hydrophilic membrane inserts, resting on top of wells that contain cultured H9 T-cells chronicall...

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Veröffentlicht in:	Journal of neuroscience methods 2011-08, Vol.200 (1), p.74-79
Hauptverfasser:	Martinez, Ricardo, Eraso, Daniel, Geffin, Rebeca, McCarthy, Micheline
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS Dementia Complex - pathology AIDS Dementia Complex - physiopathology AIDS Dementia Complex - virology Brain - pathology Brain - virology Brain slice preparation Cell culture Cell Culture Techniques - methods Cell death Cell differentiation Cell proliferation Coculture Techniques - methods Confocal microscopy Fetus - cytology Fetuses Glial fibrillary acidic protein Gliosis HIV HIV-1 - growth & development HIV-1 - physiology Human immunodeficiency virus 1 Humans Lymphocytes T Microenvironments Nervous system Nestin Neural stem cells Neuroepithelial progenitor cells Organ Culture Techniques - methods Organotypic slice culture propidium iodide Replication Stains Virology - methods Virus Replication - physiology
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