Bead size effects on protein-mediated DNA looping in tethered-particle motion experiments

Tethered particle motion (TPM) has become an important tool for single‐molecule studies of biomolecules; however, concerns remain that the method may alter the dynamics of the biophysical process under study. We investigate the effect of the attached microsphere on an illustrative biological example...

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Veröffentlicht in:	Biopolymers 2011-02, Vol.95 (2), p.144-150
Hauptverfasser:	Milstein, J. N., Chen, Y. F., Meiners, J.-C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Beads Biomolecules Biophysical Phenomena Biopolymers Deoxyribonucleic acid DNA - chemistry DNA looping Dynamical systems Dynamics Hydrodynamics In Vitro Techniques Kinetics Lac Repressors - chemistry Metal Nanoparticles Microspheres Models, Molecular Motion Nucleic Acid Conformation Particle Size Proteins - chemistry Reproduction single molecule techniques TPM
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