Feed development for fed-batch CHO production process by semisteady state analysis

Semisteady state cultures are useful for studying cell physiology and facilitating media development. Two semisteady states with a viable cell density of 5.5 million cells/mL were obtained in CHO cell cultures and compared with a fed‐batch mode control. In the first semisteady state, the culture was...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology progress 2010-05, Vol.26 (3), p.797-804
Hauptverfasser:	Khattak, Sarwat F., Xing, Zizhuo, Kenty, Brian, Koyrakh, Inna, Li, Zheng Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia - metabolism Animals Biological and medical sciences Bioreactors Biotechnology Cell Count cell culture Cell Culture Techniques - methods Cell Survival CHO Cells - cytology CHO Cells - metabolism Cricetinae Cricetulus Culture Media feeding strategy and continuous mode Fundamental and applied biological sciences. Psychology Glucose - metabolism glucose and glutamine metabolism Glutamine - metabolism Lactic Acid - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!