Label-free identification of bacterial microcolonies via elastic scattering
Label-free microcolony identification via elastic light scattering was investigated for three different genera: Salmonella enterica serovar Montevideo, Listeria monocytogenes F4244, and Escherichia coli DH5α. Microcolonies were defined as bacterial colonies in their late-lag phase to early-exponenti...
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| Veröffentlicht in: | Biotechnology and bioengineering 2011-03, Vol.108 (3), p.637-644 |
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| creator | Bae, Euiwon Bai, Nan Aroonnual, Amornrat Bhunia, Arun K Hirleman, E. Daniel |
| description | Label-free microcolony identification via elastic light scattering was investigated for three different genera: Salmonella enterica serovar Montevideo, Listeria monocytogenes F4244, and Escherichia coli DH5α. Microcolonies were defined as bacterial colonies in their late-lag phase to early-exponential phase with the diameter range of 100-200 µm. To link biophysical characteristics with corresponding scattering patterns, a phase contrast microscope and a confocal displacement meter were used to measure the colony diameter and its 3D height profile. The results indicated that the growth characteristics of microcolonies were encoded in their morphologies which correlated to the characteristic diffraction patterns. Proposed methodology was able to classify three genera based on comprehensive phenotypic map which incorporated growth speed, ring count, and colony diameter. While the proposed method illustrated the possibility of discriminating microcolonies in their early growth stage, more thorough biophysical understanding is needed to expand the technology to other species. Biotechnol. Bioeng. 2011; 108:637-644. |
| doi_str_mv | 10.1002/bit.22980 |
| format | Article |
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Daniel</creator><creatorcontrib>Bae, Euiwon ; Bai, Nan ; Aroonnual, Amornrat ; Bhunia, Arun K ; Hirleman, E. Daniel</creatorcontrib><description>Label-free microcolony identification via elastic light scattering was investigated for three different genera: Salmonella enterica serovar Montevideo, Listeria monocytogenes F4244, and Escherichia coli DH5α. Microcolonies were defined as bacterial colonies in their late-lag phase to early-exponential phase with the diameter range of 100-200 µm. To link biophysical characteristics with corresponding scattering patterns, a phase contrast microscope and a confocal displacement meter were used to measure the colony diameter and its 3D height profile. The results indicated that the growth characteristics of microcolonies were encoded in their morphologies which correlated to the characteristic diffraction patterns. Proposed methodology was able to classify three genera based on comprehensive phenotypic map which incorporated growth speed, ring count, and colony diameter. While the proposed method illustrated the possibility of discriminating microcolonies in their early growth stage, more thorough biophysical understanding is needed to expand the technology to other species. Biotechnol. Bioeng. 2011; 108:637-644.</description><identifier>ISSN: 0006-3592</identifier><identifier>ISSN: 1097-0290</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.22980</identifier><identifier>PMID: 21246511</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques - methods ; Bacteriology ; Biological and medical sciences ; Biophysics ; Biotechnology ; colony morphology ; E coli ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - growth & development ; Fundamental and applied biological sciences. Psychology ; Identification ; Light ; Light scattering ; Listeria monocytogenes ; Listeria monocytogenes - classification ; Listeria monocytogenes - growth & development ; Microbiology ; microcolony ; Microscopy, Phase-Contrast - methods ; Morphology ; Salmonella ; Salmonella enterica ; Salmonella enterica - classification ; Salmonella enterica - growth & development</subject><ispartof>Biotechnology and bioengineering, 2011-03, Vol.108 (3), p.637-644</ispartof><rights>Copyright © 2010 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Mar 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4750-1a333e9e26244dba120731852f9ed79db5994dcbd55360a2758125bb9789cf483</citedby><cites>FETCH-LOGICAL-c4750-1a333e9e26244dba120731852f9ed79db5994dcbd55360a2758125bb9789cf483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.22980$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.22980$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23842553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21246511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bae, Euiwon</creatorcontrib><creatorcontrib>Bai, Nan</creatorcontrib><creatorcontrib>Aroonnual, Amornrat</creatorcontrib><creatorcontrib>Bhunia, Arun K</creatorcontrib><creatorcontrib>Hirleman, E. Daniel</creatorcontrib><title>Label-free identification of bacterial microcolonies via elastic scattering</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Label-free microcolony identification via elastic light scattering was investigated for three different genera: Salmonella enterica serovar Montevideo, Listeria monocytogenes F4244, and Escherichia coli DH5α. Microcolonies were defined as bacterial colonies in their late-lag phase to early-exponential phase with the diameter range of 100-200 µm. To link biophysical characteristics with corresponding scattering patterns, a phase contrast microscope and a confocal displacement meter were used to measure the colony diameter and its 3D height profile. The results indicated that the growth characteristics of microcolonies were encoded in their morphologies which correlated to the characteristic diffraction patterns. Proposed methodology was able to classify three genera based on comprehensive phenotypic map which incorporated growth speed, ring count, and colony diameter. While the proposed method illustrated the possibility of discriminating microcolonies in their early growth stage, more thorough biophysical understanding is needed to expand the technology to other species. Biotechnol. Bioeng. 2011; 108:637-644.</description><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques - methods</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>colony morphology</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - growth & development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Identification</subject><subject>Light</subject><subject>Light scattering</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeria monocytogenes - growth & development</subject><subject>Microbiology</subject><subject>microcolony</subject><subject>Microscopy, Phase-Contrast - methods</subject><subject>Morphology</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - classification</subject><subject>Salmonella enterica - growth & development</subject><issn>0006-3592</issn><issn>1097-0290</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0V1rFDEUBuAgil2rF_4BHQQpXkybz0lyqYtdS9eK2NLLkGSSkpqdtMmstv_ebGdboSBehcBz3uScA8BrBPcRhPjAhHEfYyngEzBDUPIWYgmfghmEsGsJk3gHvCjlsl656LrnYAcjTDuG0AwcL7VxsfXZuSb0bhiDD1aPIQ1N8o3RdnQ56Nisgs3JppiG4ErzK-jGRV3GYJtS-QYNFy_BM69jca-25y44O_x8Ov_SLr8tjuYfl62lnMEWaUKIkw53mNLeaIQhJ0gw7KXruewNk5L21vSMkQ5qzJlAmBkjuZDWU0F2wd6Ue5XT9dqVUa1CsS5GPbi0LkoIAWsEI_-XlPGu41JW-e6RvEzrPNQ2NggJKjiu6MOE6ixKyc6rqxxWOt8qBNVmE6puQt1toto328C1Wbn-Qd6PvoL3W6DrCKPPerCh_HVEUMzuejiY3O8Q3e2_X1Sfjk7vn26nilBGd_NQofNP1XHCmTo_WSg5X5x8_c7O1WH1byfvdVL6ItdfnP3AEBGIZA1kkPwBAha1pQ</recordid><startdate>201103</startdate><enddate>201103</enddate><creator>Bae, Euiwon</creator><creator>Bai, Nan</creator><creator>Aroonnual, Amornrat</creator><creator>Bhunia, Arun K</creator><creator>Hirleman, E. 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Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4750-1a333e9e26244dba120731852f9ed79db5994dcbd55360a2758125bb9789cf483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques - methods</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>colony morphology</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - growth & development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Identification</topic><topic>Light</topic><topic>Light scattering</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeria monocytogenes - growth & development</topic><topic>Microbiology</topic><topic>microcolony</topic><topic>Microscopy, Phase-Contrast - methods</topic><topic>Morphology</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - classification</topic><topic>Salmonella enterica - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bae, Euiwon</creatorcontrib><creatorcontrib>Bai, Nan</creatorcontrib><creatorcontrib>Aroonnual, Amornrat</creatorcontrib><creatorcontrib>Bhunia, Arun K</creatorcontrib><creatorcontrib>Hirleman, E. 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Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-free identification of bacterial microcolonies via elastic scattering</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2011-03</date><risdate>2011</risdate><volume>108</volume><issue>3</issue><spage>637</spage><epage>644</epage><pages>637-644</pages><issn>0006-3592</issn><issn>1097-0290</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Label-free microcolony identification via elastic light scattering was investigated for three different genera: Salmonella enterica serovar Montevideo, Listeria monocytogenes F4244, and Escherichia coli DH5α. Microcolonies were defined as bacterial colonies in their late-lag phase to early-exponential phase with the diameter range of 100-200 µm. 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| subjects | Bacteria Bacteriological methods and techniques used in bacteriology Bacteriological Techniques - methods Bacteriology Biological and medical sciences Biophysics Biotechnology colony morphology E coli Escherichia coli Escherichia coli - classification Escherichia coli - growth & development Fundamental and applied biological sciences. Psychology Identification Light Light scattering Listeria monocytogenes Listeria monocytogenes - classification Listeria monocytogenes - growth & development Microbiology microcolony Microscopy, Phase-Contrast - methods Morphology Salmonella Salmonella enterica Salmonella enterica - classification Salmonella enterica - growth & development |
| title | Label-free identification of bacterial microcolonies via elastic scattering |
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