Development of new positive-selection RIVET tools: Detection of induced promoters by the excision-based transcriptional activation of an aacCI (Gm R)– gfp fusion

RIVET ( Recombination Based in vivo Expression Technology) is a powerful genetic tool originally conceived for the identification of genes induced in complex biological niches where conventional transcriptomics is difficult to use. With a broader application, genetic recombination-based technologies...

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Veröffentlicht in:	Journal of biotechnology 2011-09, Vol.155 (2), p.147-155
Hauptverfasser:	Lozano, M.J., Salas, M.E., Giusti, M.A., Draghi, W.O., Torres Tejerizo, G.A., Martini, M.C., Del Papa, M.F., Pistorio, M., Lagares, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	antibiotic resistance Bacteria–host interaction Biological and medical sciences Biosensing Techniques - methods Biosensor Biosensors Biotechnology clones Drug Resistance, Bacterial - genetics Escherichia coli Fundamental and applied biological sciences. Psychology Gene Expression Profiling - methods Gene Expression Regulation, Bacterial - genetics Gene Library genes Gram-negative bacteria Green Fluorescent Proteins hosts IVET Methods. Procedures. Technologies niches phenotype plasmids Plasmids - genetics Positive-selection Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - metabolism Recombination, Genetic - genetics regulatory proteins RIVET salt stress Sinorhizobium meliloti Sinorhizobium meliloti - genetics Sinorhizobium meliloti - metabolism soil bacteria transcription (genetics) transcription factors transcriptional activation Transcriptional Activation - genetics transcriptomics Transposon Resolvases - metabolism Various methods and equipments
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