EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin
Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout Oncorhynchus mykiss peritoneal cavity and...
Gespeichert in:
| Veröffentlicht in: | Gene 2011-10, Vol.485 (1), p.40-45 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 45 |
|---|---|
| container_issue | 1 |
| container_start_page | 40 |
| container_title | Gene |
| container_volume | 485 |
| creator | Kondo, Hidehiro Suga, Ryota Suda, Satomitsu Nozaki, Reiko Hirono, Ikuo Nagasaka, Reiko Kaneko, Gen Ushio, Hideki Watabe, Shugo |
| description | Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout
Oncorhynchus mykiss peritoneal cavity and searched for genes possibly related to lipid metabolism. Large numbers of ESTs encoded digestive enzymes and hormones usually found in the pancreas in higher vertebrates, consistent with the fact that pancreatic cells are dispersed in the adipose tissue. Many ESTs encoded apolipoprotein C-I, fatty acid-binding proteins and lymphocyte G0/G1 switch protein 2, which function in lipid transport, fatty acid accumulation and adipocyte differentiation, respectively. None of the ESTs encoded adipokines. We therefore obtained a cDNA encoding adiponectin, an adipokine that regulates oxidation of glucose and lipids in peripheral tissues, using rainbow trout ESTs in the public database. Real-time RT-PCR analyses revealed that its transcript levels were high in muscle and quite low in adipose tissue. These results strongly suggest that adipocytes of rainbow trout and possibly other fish species, unlike those of mammals, are not involved in the production of adipokines. |
| doi_str_mv | 10.1016/j.gene.2011.05.035 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_888098034</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378111911002733</els_id><sourcerecordid>883847602</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-133fbe4a1a3586fa888b58264b66fb7b7af912f4a51a075efb4b0290aad5cc4a3</originalsourceid><addsrcrecordid>eNqNkUFvFCEYhonR2LX6Bzzo3DzN-AHDwCReTNOqSZMe2p7JBwMt6y6sMFOz_76s23o0cvkuz_uEvC8h7yl0FOjwed3dueg6BpR2IDrg4gVZUSXHFoCrl2QFXKqWUjqekDelrKE-IdhrcsKopAykXJH1-fVNgxE3-xJKk2KDU9il4po5lLK4JvkmY4gm_W7mnJa5uYo25ft9tPdLabb7nxWr-emZn0KZczDLHKqrhv_oorNziG_JK4-b4t493VNye3F-c_a9vbz69uPs62VrBaNzSzn3xvVIkQs1eFRKGaHY0Jth8EYaiX6kzPcoKIIUzpveABsBcRLW9shPyaejd5fTr8WVWW9DsW6zwejSUnQVwqiA9_9BctXLAVgl2ZG0OZWSnde7HLaY95qCPoyh1_owhj6MoUHoOkYNfXjSL2brpr-R5_Yr8PEIeEwa73Io-va6GkQdisue00p8ORKuFvYQXNbFBhetm0KupeophX_94BFvtaXe</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>883847602</pqid></control><display><type>article</type><title>EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Kondo, Hidehiro ; Suga, Ryota ; Suda, Satomitsu ; Nozaki, Reiko ; Hirono, Ikuo ; Nagasaka, Reiko ; Kaneko, Gen ; Ushio, Hideki ; Watabe, Shugo</creator><creatorcontrib>Kondo, Hidehiro ; Suga, Ryota ; Suda, Satomitsu ; Nozaki, Reiko ; Hirono, Ikuo ; Nagasaka, Reiko ; Kaneko, Gen ; Ushio, Hideki ; Watabe, Shugo</creatorcontrib><description>Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout
Oncorhynchus mykiss peritoneal cavity and searched for genes possibly related to lipid metabolism. Large numbers of ESTs encoded digestive enzymes and hormones usually found in the pancreas in higher vertebrates, consistent with the fact that pancreatic cells are dispersed in the adipose tissue. Many ESTs encoded apolipoprotein C-I, fatty acid-binding proteins and lymphocyte G0/G1 switch protein 2, which function in lipid transport, fatty acid accumulation and adipocyte differentiation, respectively. None of the ESTs encoded adipokines. We therefore obtained a cDNA encoding adiponectin, an adipokine that regulates oxidation of glucose and lipids in peripheral tissues, using rainbow trout ESTs in the public database. Real-time RT-PCR analyses revealed that its transcript levels were high in muscle and quite low in adipose tissue. These results strongly suggest that adipocytes of rainbow trout and possibly other fish species, unlike those of mammals, are not involved in the production of adipokines.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2011.05.035</identifier><identifier>PMID: 21712077</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adipocytes ; Adiponectin ; Adiponectin - metabolism ; Adipose ; adipose tissue ; Adipose Tissue - metabolism ; Animals ; apolipoprotein C-I ; complementary DNA ; digestive enzymes ; energy metabolism ; Expressed sequence tag ; Expressed Sequence Tags ; fatty acid-binding proteins ; fatty acids ; fish ; genes ; glucose ; lipid metabolism ; Lipid Metabolism - genetics ; mammals ; muscles ; New generation sequencer ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; oxidation ; pancreas ; Rainbow trout ; reverse transcriptase polymerase chain reaction ; Tissue Distribution</subject><ispartof>Gene, 2011-10, Vol.485 (1), p.40-45</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-133fbe4a1a3586fa888b58264b66fb7b7af912f4a51a075efb4b0290aad5cc4a3</citedby><cites>FETCH-LOGICAL-c521t-133fbe4a1a3586fa888b58264b66fb7b7af912f4a51a075efb4b0290aad5cc4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2011.05.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21712077$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kondo, Hidehiro</creatorcontrib><creatorcontrib>Suga, Ryota</creatorcontrib><creatorcontrib>Suda, Satomitsu</creatorcontrib><creatorcontrib>Nozaki, Reiko</creatorcontrib><creatorcontrib>Hirono, Ikuo</creatorcontrib><creatorcontrib>Nagasaka, Reiko</creatorcontrib><creatorcontrib>Kaneko, Gen</creatorcontrib><creatorcontrib>Ushio, Hideki</creatorcontrib><creatorcontrib>Watabe, Shugo</creatorcontrib><title>EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin</title><title>Gene</title><addtitle>Gene</addtitle><description>Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout
Oncorhynchus mykiss peritoneal cavity and searched for genes possibly related to lipid metabolism. Large numbers of ESTs encoded digestive enzymes and hormones usually found in the pancreas in higher vertebrates, consistent with the fact that pancreatic cells are dispersed in the adipose tissue. Many ESTs encoded apolipoprotein C-I, fatty acid-binding proteins and lymphocyte G0/G1 switch protein 2, which function in lipid transport, fatty acid accumulation and adipocyte differentiation, respectively. None of the ESTs encoded adipokines. We therefore obtained a cDNA encoding adiponectin, an adipokine that regulates oxidation of glucose and lipids in peripheral tissues, using rainbow trout ESTs in the public database. Real-time RT-PCR analyses revealed that its transcript levels were high in muscle and quite low in adipose tissue. These results strongly suggest that adipocytes of rainbow trout and possibly other fish species, unlike those of mammals, are not involved in the production of adipokines.</description><subject>adipocytes</subject><subject>Adiponectin</subject><subject>Adiponectin - metabolism</subject><subject>Adipose</subject><subject>adipose tissue</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>apolipoprotein C-I</subject><subject>complementary DNA</subject><subject>digestive enzymes</subject><subject>energy metabolism</subject><subject>Expressed sequence tag</subject><subject>Expressed Sequence Tags</subject><subject>fatty acid-binding proteins</subject><subject>fatty acids</subject><subject>fish</subject><subject>genes</subject><subject>glucose</subject><subject>lipid metabolism</subject><subject>Lipid Metabolism - genetics</subject><subject>mammals</subject><subject>muscles</subject><subject>New generation sequencer</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>oxidation</subject><subject>pancreas</subject><subject>Rainbow trout</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Tissue Distribution</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFvFCEYhonR2LX6Bzzo3DzN-AHDwCReTNOqSZMe2p7JBwMt6y6sMFOz_76s23o0cvkuz_uEvC8h7yl0FOjwed3dueg6BpR2IDrg4gVZUSXHFoCrl2QFXKqWUjqekDelrKE-IdhrcsKopAykXJH1-fVNgxE3-xJKk2KDU9il4po5lLK4JvkmY4gm_W7mnJa5uYo25ft9tPdLabb7nxWr-emZn0KZczDLHKqrhv_oorNziG_JK4-b4t493VNye3F-c_a9vbz69uPs62VrBaNzSzn3xvVIkQs1eFRKGaHY0Jth8EYaiX6kzPcoKIIUzpveABsBcRLW9shPyaejd5fTr8WVWW9DsW6zwejSUnQVwqiA9_9BctXLAVgl2ZG0OZWSnde7HLaY95qCPoyh1_owhj6MoUHoOkYNfXjSL2brpr-R5_Yr8PEIeEwa73Io-va6GkQdisue00p8ORKuFvYQXNbFBhetm0KupeophX_94BFvtaXe</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Kondo, Hidehiro</creator><creator>Suga, Ryota</creator><creator>Suda, Satomitsu</creator><creator>Nozaki, Reiko</creator><creator>Hirono, Ikuo</creator><creator>Nagasaka, Reiko</creator><creator>Kaneko, Gen</creator><creator>Ushio, Hideki</creator><creator>Watabe, Shugo</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20111001</creationdate><title>EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin</title><author>Kondo, Hidehiro ; Suga, Ryota ; Suda, Satomitsu ; Nozaki, Reiko ; Hirono, Ikuo ; Nagasaka, Reiko ; Kaneko, Gen ; Ushio, Hideki ; Watabe, Shugo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-133fbe4a1a3586fa888b58264b66fb7b7af912f4a51a075efb4b0290aad5cc4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adipocytes</topic><topic>Adiponectin</topic><topic>Adiponectin - metabolism</topic><topic>Adipose</topic><topic>adipose tissue</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>apolipoprotein C-I</topic><topic>complementary DNA</topic><topic>digestive enzymes</topic><topic>energy metabolism</topic><topic>Expressed sequence tag</topic><topic>Expressed Sequence Tags</topic><topic>fatty acid-binding proteins</topic><topic>fatty acids</topic><topic>fish</topic><topic>genes</topic><topic>glucose</topic><topic>lipid metabolism</topic><topic>Lipid Metabolism - genetics</topic><topic>mammals</topic><topic>muscles</topic><topic>New generation sequencer</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>oxidation</topic><topic>pancreas</topic><topic>Rainbow trout</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kondo, Hidehiro</creatorcontrib><creatorcontrib>Suga, Ryota</creatorcontrib><creatorcontrib>Suda, Satomitsu</creatorcontrib><creatorcontrib>Nozaki, Reiko</creatorcontrib><creatorcontrib>Hirono, Ikuo</creatorcontrib><creatorcontrib>Nagasaka, Reiko</creatorcontrib><creatorcontrib>Kaneko, Gen</creatorcontrib><creatorcontrib>Ushio, Hideki</creatorcontrib><creatorcontrib>Watabe, Shugo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kondo, Hidehiro</au><au>Suga, Ryota</au><au>Suda, Satomitsu</au><au>Nozaki, Reiko</au><au>Hirono, Ikuo</au><au>Nagasaka, Reiko</au><au>Kaneko, Gen</au><au>Ushio, Hideki</au><au>Watabe, Shugo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>485</volume><issue>1</issue><spage>40</spage><epage>45</epage><pages>40-45</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout
Oncorhynchus mykiss peritoneal cavity and searched for genes possibly related to lipid metabolism. Large numbers of ESTs encoded digestive enzymes and hormones usually found in the pancreas in higher vertebrates, consistent with the fact that pancreatic cells are dispersed in the adipose tissue. Many ESTs encoded apolipoprotein C-I, fatty acid-binding proteins and lymphocyte G0/G1 switch protein 2, which function in lipid transport, fatty acid accumulation and adipocyte differentiation, respectively. None of the ESTs encoded adipokines. We therefore obtained a cDNA encoding adiponectin, an adipokine that regulates oxidation of glucose and lipids in peripheral tissues, using rainbow trout ESTs in the public database. Real-time RT-PCR analyses revealed that its transcript levels were high in muscle and quite low in adipose tissue. These results strongly suggest that adipocytes of rainbow trout and possibly other fish species, unlike those of mammals, are not involved in the production of adipokines.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21712077</pmid><doi>10.1016/j.gene.2011.05.035</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 2011-10, Vol.485 (1), p.40-45 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_888098034 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | adipocytes Adiponectin Adiponectin - metabolism Adipose adipose tissue Adipose Tissue - metabolism Animals apolipoprotein C-I complementary DNA digestive enzymes energy metabolism Expressed sequence tag Expressed Sequence Tags fatty acid-binding proteins fatty acids fish genes glucose lipid metabolism Lipid Metabolism - genetics mammals muscles New generation sequencer Oncorhynchus mykiss Oncorhynchus mykiss - genetics oxidation pancreas Rainbow trout reverse transcriptase polymerase chain reaction Tissue Distribution |
| title | EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T01%3A56%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=EST%20analysis%20on%20adipose%20tissue%20of%20rainbow%20trout%20Oncorhynchus%20mykiss%20and%20tissue%20distribution%20of%20adiponectin&rft.jtitle=Gene&rft.au=Kondo,%20Hidehiro&rft.date=2011-10-01&rft.volume=485&rft.issue=1&rft.spage=40&rft.epage=45&rft.pages=40-45&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/j.gene.2011.05.035&rft_dat=%3Cproquest_cross%3E883847602%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=883847602&rft_id=info:pmid/21712077&rft_els_id=S0378111911002733&rfr_iscdi=true |