Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system
The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis‐regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large‐scale analysis of enhancer elements, site‐specifically integrated into the Drosophila genome. This s...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2010-07, Vol.48 (7), p.452-456 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Boy, Aurelia L. Zhai, Zongzhao Habring-Müller, Anette Kussler-Schneider, Yvonne Kaspar, Petra Lohmann, Ingrid |
| description | The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis‐regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large‐scale analysis of enhancer elements, site‐specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high‐throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site‐specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452–456, 2010. © 2010 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dvg.20637 |
| format | Article |
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Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452–456, 2010. © 2010 Wiley‐Liss, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>ISSN: 1526-968X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/dvg.20637</identifier><identifier>PMID: 20506180</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Animals, Genetically Modified ; Binding Sites ; Cloning, Molecular - methods ; Drosophila ; Drosophila melanogaster - genetics ; eGFP ; Enhancer Elements, Genetic - genetics ; Gateway system ; Gene Expression Regulation - genetics ; Gene Transfer Techniques ; Genes, Reporter ; Genetic Vectors ; High-Throughput Screening Assays - methods ; Integrases - genetics ; mCherry ; PhiC31 integrase ; Plasmids ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Recombination, Genetic ; reporter lines ; transgenesis ; Venus</subject><ispartof>Genesis (New York, N.Y. : 2000), 2010-07, Vol.48 (7), p.452-456</ispartof><rights>Copyright © 2010 Wiley‐Liss, Inc.</rights><rights>(c) 2010 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4607-68b6b246794dcf1a3c3d829dd6e498fa5b2a39d0add5d037ca70e3b9ff25e7db3</citedby><cites>FETCH-LOGICAL-c4607-68b6b246794dcf1a3c3d829dd6e498fa5b2a39d0add5d037ca70e3b9ff25e7db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvg.20637$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvg.20637$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20506180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boy, Aurelia L.</creatorcontrib><creatorcontrib>Zhai, Zongzhao</creatorcontrib><creatorcontrib>Habring-Müller, Anette</creatorcontrib><creatorcontrib>Kussler-Schneider, Yvonne</creatorcontrib><creatorcontrib>Kaspar, Petra</creatorcontrib><creatorcontrib>Lohmann, Ingrid</creatorcontrib><title>Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis‐regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large‐scale analysis of enhancer elements, site‐specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high‐throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site‐specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452–456, 2010. © 2010 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Binding Sites</subject><subject>Cloning, Molecular - methods</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - genetics</subject><subject>eGFP</subject><subject>Enhancer Elements, Genetic - genetics</subject><subject>Gateway system</subject><subject>Gene Expression Regulation - genetics</subject><subject>Gene Transfer Techniques</subject><subject>Genes, Reporter</subject><subject>Genetic Vectors</subject><subject>High-Throughput Screening Assays - methods</subject><subject>Integrases - genetics</subject><subject>mCherry</subject><subject>PhiC31 integrase</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Recombination, Genetic</subject><subject>reporter lines</subject><subject>transgenesis</subject><subject>Venus</subject><issn>1526-954X</issn><issn>1526-968X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EoqVw4AWQb4hDWsdOHOeIFtgiraBIsPRmOfY4MWTjYDvAvgMPjdvt9oY4zRy--UYzP0LPS3JeEkIvzM_-nBLOmgfotKwpL1ourh8e-7q6PkFPYvxGCKkFpY_RCSU14aUgp-jPFnTyIWLrAwZrnXYwJawmgwfXD0Uagl_6YV4S1n6KKSw6OT9hb7EdFx8g6hveBB_9PLhR4QCzDwmycolu6nEaAF8NbsVKHF2CIs6gXd6D3ZSgD-pWF_cxwe4pemTVGOHZXT1DX969_by6LDYf1-9XrzeFrjhpCi463tGKN21ltC0V08wI2hrDoWqFVXVHFWsNUcbUhrBGq4YA61praQ2N6dgZennwzsH_WCAmuXP5jnFUE_glSiEEaTln5X_JhrFWtISITL46kDq_Igawcg5up8JelkTepCRzSvI2pcy-uLMu3Q7MPXmMJQMXB-CXG2H_b5N8s10flcVhwuVH_r6fUOG75A1ravn1w1puPl1WV_kwuWV_AbVmrz4</recordid><startdate>201007</startdate><enddate>201007</enddate><creator>Boy, Aurelia L.</creator><creator>Zhai, Zongzhao</creator><creator>Habring-Müller, Anette</creator><creator>Kussler-Schneider, Yvonne</creator><creator>Kaspar, Petra</creator><creator>Lohmann, Ingrid</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201007</creationdate><title>Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system</title><author>Boy, Aurelia L. ; Zhai, Zongzhao ; Habring-Müller, Anette ; Kussler-Schneider, Yvonne ; Kaspar, Petra ; Lohmann, Ingrid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4607-68b6b246794dcf1a3c3d829dd6e498fa5b2a39d0add5d037ca70e3b9ff25e7db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Binding Sites</topic><topic>Cloning, Molecular - methods</topic><topic>Drosophila</topic><topic>Drosophila melanogaster - genetics</topic><topic>eGFP</topic><topic>Enhancer Elements, Genetic - genetics</topic><topic>Gateway system</topic><topic>Gene Expression Regulation - genetics</topic><topic>Gene Transfer Techniques</topic><topic>Genes, Reporter</topic><topic>Genetic Vectors</topic><topic>High-Throughput Screening Assays - methods</topic><topic>Integrases - genetics</topic><topic>mCherry</topic><topic>PhiC31 integrase</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Recombination, Genetic</topic><topic>reporter lines</topic><topic>transgenesis</topic><topic>Venus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boy, Aurelia L.</creatorcontrib><creatorcontrib>Zhai, Zongzhao</creatorcontrib><creatorcontrib>Habring-Müller, Anette</creatorcontrib><creatorcontrib>Kussler-Schneider, Yvonne</creatorcontrib><creatorcontrib>Kaspar, Petra</creatorcontrib><creatorcontrib>Lohmann, Ingrid</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boy, Aurelia L.</au><au>Zhai, Zongzhao</au><au>Habring-Müller, Anette</au><au>Kussler-Schneider, Yvonne</au><au>Kaspar, Petra</au><au>Lohmann, Ingrid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2010-07</date><risdate>2010</risdate><volume>48</volume><issue>7</issue><spage>452</spage><epage>456</epage><pages>452-456</pages><issn>1526-954X</issn><issn>1526-968X</issn><eissn>1526-968X</eissn><abstract>The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis‐regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large‐scale analysis of enhancer elements, site‐specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high‐throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site‐specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452–456, 2010. © 2010 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20506180</pmid><doi>10.1002/dvg.20637</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Animals, Genetically Modified Binding Sites Cloning, Molecular - methods Drosophila Drosophila melanogaster - genetics eGFP Enhancer Elements, Genetic - genetics Gateway system Gene Expression Regulation - genetics Gene Transfer Techniques Genes, Reporter Genetic Vectors High-Throughput Screening Assays - methods Integrases - genetics mCherry PhiC31 integrase Plasmids Promoter Regions, Genetic Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombination, Genetic reporter lines transgenesis Venus |
| title | Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system |
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