Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation
The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patient...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2011-02, Vol.30 (2), p.181-191 |
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| creator | Afanas'ev, M. V Ikryannikova, L. N Il'ina, E. N Kuz'min, A. V Larionova, E. E Smirnova, T. G Chernousova, L. N Govorun, V. M |
| description | The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. Comparison of the 24-VNTR locus typing and spoligotyping data with revealed resistance-associated mutation allows us to make a suggestion that the active transmission of MDR strains and the independent appearance of drug resistance during chemotherapy occurred in the studied population simultaneously. |
| doi_str_mv | 10.1007/s10096-010-1067-z |
| format | Article |
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V ; Ikryannikova, L. N ; Il'ina, E. N ; Kuz'min, A. V ; Larionova, E. E ; Smirnova, T. G ; Chernousova, L. N ; Govorun, V. M</creator><creatorcontrib>Afanas'ev, M. V ; Ikryannikova, L. N ; Il'ina, E. N ; Kuz'min, A. V ; Larionova, E. E ; Smirnova, T. G ; Chernousova, L. N ; Govorun, V. M</creatorcontrib><description>The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. Comparison of the 24-VNTR locus typing and spoligotyping data with revealed resistance-associated mutation allows us to make a suggestion that the active transmission of MDR strains and the independent appearance of drug resistance during chemotherapy occurred in the studied population simultaneously.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-010-1067-z</identifier><identifier>PMID: 20941520</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Adolescent ; Adult ; Aged ; Amino Acid Substitution ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Biomedical and Life Sciences ; Biomedicine ; Catalase - genetics ; DNA-Directed RNA Polymerases ; Drug resistance ; Female ; Gene loci ; Genetic Variation ; Genotype ; Heterogeneity ; Humans ; Internal Medicine ; Male ; Medical Microbiology ; Middle Aged ; Molecular Epidemiology ; Molecular Typing ; Moscow - epidemiology ; Mutation ; Mutation, Missense ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - classification ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Population ; Sputum - microbiology ; Tuberculosis ; Tuberculosis - epidemiology ; Tuberculosis - microbiology ; Young Adult</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2011-02, Vol.30 (2), p.181-191</ispartof><rights>Springer-Verlag 2010</rights><rights>Springer-Verlag 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-3a802a25a44f6111ed5e57cdeeed667b7971ae826c63157a02e4b34f49662ad73</citedby><cites>FETCH-LOGICAL-c426t-3a802a25a44f6111ed5e57cdeeed667b7971ae826c63157a02e4b34f49662ad73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-010-1067-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-010-1067-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20941520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Afanas'ev, M. V</creatorcontrib><creatorcontrib>Ikryannikova, L. N</creatorcontrib><creatorcontrib>Il'ina, E. N</creatorcontrib><creatorcontrib>Kuz'min, A. V</creatorcontrib><creatorcontrib>Larionova, E. E</creatorcontrib><creatorcontrib>Smirnova, T. G</creatorcontrib><creatorcontrib>Chernousova, L. N</creatorcontrib><creatorcontrib>Govorun, V. M</creatorcontrib><title>Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. 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V</au><au>Ikryannikova, L. N</au><au>Il'ina, E. N</au><au>Kuz'min, A. V</au><au>Larionova, E. E</au><au>Smirnova, T. G</au><au>Chernousova, L. N</au><au>Govorun, V. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>30</volume><issue>2</issue><spage>181</spage><epage>191</epage><pages>181-191</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. Comparison of the 24-VNTR locus typing and spoligotyping data with revealed resistance-associated mutation allows us to make a suggestion that the active transmission of MDR strains and the independent appearance of drug resistance during chemotherapy occurred in the studied population simultaneously.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20941520</pmid><doi>10.1007/s10096-010-1067-z</doi><tpages>11</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Amino Acid Substitution Bacterial Proteins - genetics Bacterial Typing Techniques Biomedical and Life Sciences Biomedicine Catalase - genetics DNA-Directed RNA Polymerases Drug resistance Female Gene loci Genetic Variation Genotype Heterogeneity Humans Internal Medicine Male Medical Microbiology Middle Aged Molecular Epidemiology Molecular Typing Moscow - epidemiology Mutation Mutation, Missense Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Population Sputum - microbiology Tuberculosis Tuberculosis - epidemiology Tuberculosis - microbiology Young Adult |
| title | Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation |
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