Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil
The mullets of the family Mugilidae, and in particular the species of the genus Mugil, are characterized by highly conserved morphological features, which hinder taxonomic classification considerably. Given this, the present study aimed to evaluate the efficiency of the 5S rDNA gene – a rapid and lo...
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| Veröffentlicht in: | Aquaculture research 2011-07, Vol.42 (8), p.1117-1122 |
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| creator | Rodrigues-Filho, Luis Fernando S da Cunha, Divino Bruno Vallinoto, Marcelo Schneider, Horacio Sampaio, Iracilda Fraga, Elmary |
| description | The mullets of the family Mugilidae, and in particular the species of the genus Mugil, are characterized by highly conserved morphological features, which hinder taxonomic classification considerably. Given this, the present study aimed to evaluate the efficiency of the 5S rDNA gene – a rapid and low cost genotyping marker – for the discrimination of Mugil species. Variations in the banding pattern permitted the reliable differentiation of four species –Mugil curema, Mugil incilis, Mugil sp. and Mugil hospes– although those of three others (Mugil liza, Mugil platanus and Mugil cephalus) were identical. This suggests that these three forms have diverged very recently, on a time scale that has limited changes in the length of the fragments of the 5S rDNA gene. The results of the study confirm the efficiency of this marker as a diagnostic tool for the identification of Mugil species, and its usefulness for population management and pisciculture, an important economic activity involving this group. |
| doi_str_mv | 10.1111/j.1365-2109.2010.02698.x |
| format | Article |
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Given this, the present study aimed to evaluate the efficiency of the 5S rDNA gene – a rapid and low cost genotyping marker – for the discrimination of Mugil species. Variations in the banding pattern permitted the reliable differentiation of four species –Mugil curema, Mugil incilis, Mugil sp. and Mugil hospes– although those of three others (Mugil liza, Mugil platanus and Mugil cephalus) were identical. This suggests that these three forms have diverged very recently, on a time scale that has limited changes in the length of the fragments of the 5S rDNA gene. 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The results of the study confirm the efficiency of this marker as a diagnostic tool for the identification of Mugil species, and its usefulness for population management and pisciculture, an important economic activity involving this group.</description><subject>Marine</subject><subject>Mugil</subject><subject>Mugil cephalus</subject><subject>Mugil incilis</subject><subject>Mugil liza</subject><subject>Mugil platanus</subject><subject>Mugilidae</subject><subject>Mullets</subject><subject>PCR bands</subject><subject>rDNA 5S</subject><subject>South America</subject><subject>species identification</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNo9Uctu2zAQFIoWaJr2H_bWk1w-RIk69GCkSVogTR9JH-iFoKmVTVcmHZJC7S_J74ayC_PCxXJmdjlTFEDJjObzbj2jvBYlo6SdMZK7hNWtnO2eFWenh-dTLUQpRPP7ZfEqxjUhtCKcnhWPX_2w32DQEcGstHUQUJtkvYOFdp11S9jqlDC4CL6HtEIQdxA-3M5hiQ5BR9DQWb10PiZrIHk_QO_DAdnZaILdWKcPgpl_58e0gnkeaI12sBmHAdNJOSuOET6PSzu8Ll70eoj45v99Xvy4ury_-FjefLn-dDG_KQ2XlSwXSOu6rxmavudEa1Hpppaya5FxJB2vqGGUsrqperJgi64zfdMRiryR1Ahi-Hnx9qi7Df5hxJjUJi-Nw6Ad-jEqKSVpRUN4Rr4_Iv_ZAfdqmz-mw15RoqYc1FpNdqvJbjXloA45qJ2af7-cqswvj3wbE-5OfB3-qrrhjVC_bq8Vu5c_r9o_XH3jT75Nj3w</recordid><startdate>201107</startdate><enddate>201107</enddate><creator>Rodrigues-Filho, Luis Fernando S</creator><creator>da Cunha, Divino Bruno</creator><creator>Vallinoto, Marcelo</creator><creator>Schneider, Horacio</creator><creator>Sampaio, Iracilda</creator><creator>Fraga, Elmary</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>7ST</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>201107</creationdate><title>Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil</title><author>Rodrigues-Filho, Luis Fernando S ; 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| subjects | Marine Mugil Mugil cephalus Mugil incilis Mugil liza Mugil platanus Mugilidae Mullets PCR bands rDNA 5S South America species identification |
| title | Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil |
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