Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut
The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a tran...
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| Veröffentlicht in: | Plant biotechnology journal 2011-10, Vol.9 (8), p.857-864 |
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| creator | Allen, Aron Islamovic, Emir Kaur, Jagdeep Gold, Scott Shah, Dilip Smith, Thomas J |
| description | The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins. |
| doi_str_mv | 10.1111/j.1467-7652.2011.00590.x |
| format | Article |
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Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.</description><identifier>ISSN: 1467-7652</identifier><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/j.1467-7652.2011.00590.x</identifier><identifier>PMID: 21303448</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Anthocyanins - metabolism ; antifungal proteins ; Biological and medical sciences ; Biotechnology ; breeding ; Cloning, Molecular ; corn ; Disease Resistance ; ears ; Fundamental and applied biological sciences. Psychology ; fungi ; gene expression ; genes ; Genes, Plant ; Genetic Vectors ; Germination ; greenhouses ; Inbreeding ; KP4 ; Microbial Sensitivity Tests - methods ; Molecular Sequence Data ; pathogens ; plant development ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Leaves - metabolism ; Plant Leaves - microbiology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - immunology ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; Promoter Regions, Genetic ; Protein Sorting Signals ; smut diseases ; stems ; Totivirus ; transgenic ; transgenic plants ; Ustilago - genetics ; Ustilago - immunology ; Ustilago - pathogenicity ; Ustilago maydis ; Ustilago zeae ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Zea mays - genetics ; Zea mays - immunology ; Zea mays - metabolism ; Zea mays - microbiology</subject><ispartof>Plant biotechnology journal, 2011-10, Vol.9 (8), p.857-864</ispartof><rights>2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2011 The Authors. 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Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.</description><subject>Amino Acid Sequence</subject><subject>Anthocyanins - metabolism</subject><subject>antifungal proteins</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>breeding</subject><subject>Cloning, Molecular</subject><subject>corn</subject><subject>Disease Resistance</subject><subject>ears</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungi</subject><subject>gene expression</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Genetic Vectors</subject><subject>Germination</subject><subject>greenhouses</subject><subject>Inbreeding</subject><subject>KP4</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Molecular Sequence Data</subject><subject>pathogens</subject><subject>plant development</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - microbiology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - immunology</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Sorting Signals</subject><subject>smut diseases</subject><subject>stems</subject><subject>Totivirus</subject><subject>transgenic</subject><subject>transgenic plants</subject><subject>Ustilago - genetics</subject><subject>Ustilago - immunology</subject><subject>Ustilago - pathogenicity</subject><subject>Ustilago maydis</subject><subject>Ustilago zeae</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Zea mays - genetics</subject><subject>Zea mays - immunology</subject><subject>Zea mays - metabolism</subject><subject>Zea mays - microbiology</subject><issn>1467-7652</issn><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1v0zAUhi3ExMbgL4BvEDdLsOPYSSRutomVfQgqrRMSN5br2qm7fBQ72Vp-_U6WUrjENz7SeV77nAchTElM4XxaxTQVWZQJnsQJoTQmhBck3rxAR_vGy3_qQ_Q6hBUhCRVcvEKHCWWEpWl-hO5nXjWhNI3TuFbut8HrSjVdwGaz9iYE15S4Wxo8azv34HwfMHSd7ZtSVXjt28645gRfT9MTrLzBS1cuqy2GpAsdkLhrsW59g0Pdd2_QgVVVMG939zG6u_gyO_8a3XyfXJ6f3kSak4JElNp8bo1KGFnMM8IzxZSgOcmpnVsrYAuSZ5RoZizjySKj0CuE1lyYQieJZsfo4_guzPerN6GTtQvaVLCYafsg8xwCYCwFMh9J7dsQvLFy7V2t_FZSIgfTciUHiXKQKAfT8tm03ED03e6Tfl6bxT74Ry0AH3aAClpVFjxrF_5yKYdt2TDD55F7dJXZ_vcAcnp2CQXEozEOws1mH1f-XoqMZVz--DaRF2dXP6fXt0JOgH8_8la1UpUeRrq7hYcZoQUvUiieAD7ysfM</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Allen, Aron</creator><creator>Islamovic, Emir</creator><creator>Kaur, Jagdeep</creator><creator>Gold, Scott</creator><creator>Shah, Dilip</creator><creator>Smith, Thomas J</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut</title><author>Allen, Aron ; Islamovic, Emir ; Kaur, Jagdeep ; Gold, Scott ; Shah, Dilip ; Smith, Thomas J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5090-11f8bfea230db7057a3a618081fbff600208710c3ef352d7118096cc56e9c22c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Sequence</topic><topic>Anthocyanins - metabolism</topic><topic>antifungal proteins</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>breeding</topic><topic>Cloning, Molecular</topic><topic>corn</topic><topic>Disease Resistance</topic><topic>ears</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungi</topic><topic>gene expression</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Genetic Vectors</topic><topic>Germination</topic><topic>greenhouses</topic><topic>Inbreeding</topic><topic>KP4</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Molecular Sequence Data</topic><topic>pathogens</topic><topic>plant development</topic><topic>Plant Diseases - immunology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - microbiology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - immunology</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Sorting Signals</topic><topic>smut diseases</topic><topic>stems</topic><topic>Totivirus</topic><topic>transgenic</topic><topic>transgenic plants</topic><topic>Ustilago - genetics</topic><topic>Ustilago - immunology</topic><topic>Ustilago - pathogenicity</topic><topic>Ustilago maydis</topic><topic>Ustilago zeae</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Zea mays - genetics</topic><topic>Zea mays - immunology</topic><topic>Zea mays - metabolism</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allen, Aron</creatorcontrib><creatorcontrib>Islamovic, Emir</creatorcontrib><creatorcontrib>Kaur, Jagdeep</creatorcontrib><creatorcontrib>Gold, Scott</creatorcontrib><creatorcontrib>Shah, Dilip</creatorcontrib><creatorcontrib>Smith, Thomas J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Allen, Aron</au><au>Islamovic, Emir</au><au>Kaur, Jagdeep</au><au>Gold, Scott</au><au>Shah, Dilip</au><au>Smith, Thomas J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2011-10</date><risdate>2011</risdate><volume>9</volume><issue>8</issue><spage>857</spage><epage>864</epage><pages>857-864</pages><issn>1467-7652</issn><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21303448</pmid><doi>10.1111/j.1467-7652.2011.00590.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Anthocyanins - metabolism antifungal proteins Biological and medical sciences Biotechnology breeding Cloning, Molecular corn Disease Resistance ears Fundamental and applied biological sciences. Psychology fungi gene expression genes Genes, Plant Genetic Vectors Germination greenhouses Inbreeding KP4 Microbial Sensitivity Tests - methods Molecular Sequence Data pathogens plant development Plant Diseases - immunology Plant Diseases - microbiology Plant Leaves - metabolism Plant Leaves - microbiology Plants, Genetically Modified - genetics Plants, Genetically Modified - immunology Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology Promoter Regions, Genetic Protein Sorting Signals smut diseases stems Totivirus transgenic transgenic plants Ustilago - genetics Ustilago - immunology Ustilago - pathogenicity Ustilago maydis Ustilago zeae Viral Proteins - genetics Viral Proteins - metabolism Zea mays - genetics Zea mays - immunology Zea mays - metabolism Zea mays - microbiology |
| title | Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut |
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