Single-molecule recognition force spectroscopy of transmembrane transporters on living cells

Atomic force microscopy (AFM) has proven to be a powerful tool in biological sciences. Its particular advantage over other high-resolution methods commonly used is that biomolecules can be investigated not only under physiological conditions but also while they perform their biological functions. Si...

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Veröffentlicht in:	Nature protocols 2011-09, Vol.6 (9), p.1443-1452
Hauptverfasser:	Puntheeranurak, Theeraporn, Neundlinger, Isabel, Kinne, Rolf K H, Hinterdorfer, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/328/1262 631/1647/527 631/92/577 Analytical Chemistry Animals Atomic force microscopy Binding Sites Biological Techniques Biomolecules Biophysics Cell Culture Techniques Cell Line Computational Biology/Bioinformatics Cricetinae Cricetulus Gene expression Life Sciences Ligands Medical research Membrane Transport Proteins - chemistry Microarrays Microscopy Microscopy, Atomic Force - methods Organic Chemistry Physiological aspects Physiology Proteins protocol Sample preparation Sodium-Glucose Transporter 1 - chemistry Spectroscopy
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