Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene
The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic anal...
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| Veröffentlicht in: | Zoological Science 2011-09, Vol.28 (9), p.671-675 |
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| container_title | Zoological Science |
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| creator | Bae, Jin-Han Ahn, Kung Nam, Gyu-Hwi Lee, Chong-Eon Park, Kyung-Do Lee, Hak-Kyo Cho, Byung-Wook Kim, Heui-Soo |
| description | The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney. |
| doi_str_mv | 10.2108/zsj.28.671 |
| format | Article |
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The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney.</description><identifier>ISSN: 0289-0003</identifier><identifier>DOI: 10.2108/zsj.28.671</identifier><identifier>PMID: 21882956</identifier><language>eng</language><publisher>Japan: UniBio Press</publisher><subject>Alternative Splicing ; alternative variants ; Animals ; ARNTL Transcription Factors - genetics ; ARNTL Transcription Factors - metabolism ; Base Sequence ; Bioinformatics ; BMAL1 ; BMAL1 protein ; Brain ; cassette exon ; Cerebellum ; Circadian rhythms ; DNA, Complementary - genetics ; exonization ; Exons ; Heart ; Horses - genetics ; Horses - metabolism ; Kidney ; Molecular Sequence Annotation ; Muscles ; ORIGINAL ARTICLES ; Polymerase chain reaction ; quantitative real-time RT-PCR</subject><ispartof>Zoological Science, 2011-09, Vol.28 (9), p.671-675</ispartof><rights>2011 Zoological Society of Japan</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b539t-1b4085ee26f8175afac8160e4762e155387a3fb1a8d9fecbf11f21c9662feda63</citedby><cites>FETCH-LOGICAL-b539t-1b4085ee26f8175afac8160e4762e155387a3fb1a8d9fecbf11f21c9662feda63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.2108/zsj.28.671$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21882956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bae, Jin-Han</creatorcontrib><creatorcontrib>Ahn, Kung</creatorcontrib><creatorcontrib>Nam, Gyu-Hwi</creatorcontrib><creatorcontrib>Lee, Chong-Eon</creatorcontrib><creatorcontrib>Park, Kyung-Do</creatorcontrib><creatorcontrib>Lee, Hak-Kyo</creatorcontrib><creatorcontrib>Cho, Byung-Wook</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><title>Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene</title><title>Zoological Science</title><addtitle>Zoolog Sci</addtitle><description>The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney.</description><subject>Alternative Splicing</subject><subject>alternative variants</subject><subject>Animals</subject><subject>ARNTL Transcription Factors - genetics</subject><subject>ARNTL Transcription Factors - metabolism</subject><subject>Base Sequence</subject><subject>Bioinformatics</subject><subject>BMAL1</subject><subject>BMAL1 protein</subject><subject>Brain</subject><subject>cassette exon</subject><subject>Cerebellum</subject><subject>Circadian rhythms</subject><subject>DNA, Complementary - genetics</subject><subject>exonization</subject><subject>Exons</subject><subject>Heart</subject><subject>Horses - genetics</subject><subject>Horses - metabolism</subject><subject>Kidney</subject><subject>Molecular Sequence Annotation</subject><subject>Muscles</subject><subject>ORIGINAL ARTICLES</subject><subject>Polymerase chain reaction</subject><subject>quantitative real-time RT-PCR</subject><issn>0289-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v2zAMhnVY0c9d9gMG3zYMSCrKkSwfs2BrByTdpTsLskIhChzJk5QG7a-fjHo99kSQfPj1kpBPQOcMqLx9Sfs5k3PRwAdySZlsZ5TS-oJcpbSnFCRwOCcXDKRkLReXZLMJPZpjr2O12umoTcboXnR2wVfBVsu--L64T1g9Ru2TiW7IaUzlHVb3ISasvm-Wa6ju0OMNObO6T_hxstfkz88fj6v72fr33a_Vcj3reN3mGXQLKjkiE1ZCw7XVRoKguGgEQ-C8lo2ubQdabluLprMAloFphWAWt1rU1-TLa98hhr9HTFkdXDLY99pjOCYlZcPpgou2kF_fJYFC0_JFA7Kg315RE0NKEa0aojvo-FwgNYqririKSVXELfDnqe-xO-D2Df2vbAEeJsBhfEvvch7U6XRSYzT43nlU-0GZ4DP6rHTMzvSoxsep8XFlHG3pNHG6pHMhlLJ3lvsHC3mgBg</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Bae, Jin-Han</creator><creator>Ahn, Kung</creator><creator>Nam, Gyu-Hwi</creator><creator>Lee, Chong-Eon</creator><creator>Park, Kyung-Do</creator><creator>Lee, Hak-Kyo</creator><creator>Cho, Byung-Wook</creator><creator>Kim, Heui-Soo</creator><general>UniBio Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene</title><author>Bae, Jin-Han ; Ahn, Kung ; Nam, Gyu-Hwi ; Lee, Chong-Eon ; Park, Kyung-Do ; Lee, Hak-Kyo ; Cho, Byung-Wook ; Kim, Heui-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b539t-1b4085ee26f8175afac8160e4762e155387a3fb1a8d9fecbf11f21c9662feda63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alternative Splicing</topic><topic>alternative variants</topic><topic>Animals</topic><topic>ARNTL Transcription Factors - genetics</topic><topic>ARNTL Transcription Factors - metabolism</topic><topic>Base Sequence</topic><topic>Bioinformatics</topic><topic>BMAL1</topic><topic>BMAL1 protein</topic><topic>Brain</topic><topic>cassette exon</topic><topic>Cerebellum</topic><topic>Circadian rhythms</topic><topic>DNA, Complementary - genetics</topic><topic>exonization</topic><topic>Exons</topic><topic>Heart</topic><topic>Horses - genetics</topic><topic>Horses - metabolism</topic><topic>Kidney</topic><topic>Molecular Sequence Annotation</topic><topic>Muscles</topic><topic>ORIGINAL ARTICLES</topic><topic>Polymerase chain reaction</topic><topic>quantitative real-time RT-PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bae, Jin-Han</creatorcontrib><creatorcontrib>Ahn, Kung</creatorcontrib><creatorcontrib>Nam, Gyu-Hwi</creatorcontrib><creatorcontrib>Lee, Chong-Eon</creatorcontrib><creatorcontrib>Park, Kyung-Do</creatorcontrib><creatorcontrib>Lee, Hak-Kyo</creatorcontrib><creatorcontrib>Cho, Byung-Wook</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Zoological Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bae, Jin-Han</au><au>Ahn, Kung</au><au>Nam, Gyu-Hwi</au><au>Lee, Chong-Eon</au><au>Park, Kyung-Do</au><au>Lee, Hak-Kyo</au><au>Cho, Byung-Wook</au><au>Kim, Heui-Soo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene</atitle><jtitle>Zoological Science</jtitle><addtitle>Zoolog Sci</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>28</volume><issue>9</issue><spage>671</spage><epage>675</epage><pages>671-675</pages><issn>0289-0003</issn><abstract>The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney.</abstract><cop>Japan</cop><pub>UniBio Press</pub><pmid>21882956</pmid><doi>10.2108/zsj.28.671</doi><tpages>5</tpages></addata></record> |
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| subjects | Alternative Splicing alternative variants Animals ARNTL Transcription Factors - genetics ARNTL Transcription Factors - metabolism Base Sequence Bioinformatics BMAL1 BMAL1 protein Brain cassette exon Cerebellum Circadian rhythms DNA, Complementary - genetics exonization Exons Heart Horses - genetics Horses - metabolism Kidney Molecular Sequence Annotation Muscles ORIGINAL ARTICLES Polymerase chain reaction quantitative real-time RT-PCR |
| title | Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene |
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