Design of Aptamer-Based Sensing Platform Using Triple-Helix Molecular Switch
For successful assay development of an aptamer-based biosensor, various design principles and strategies, including a highly selective molecular recognition element and a novel signal transduction mechanism, have to be engineered together. Herein, we report a new type of aptamer-based sensing platfo...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2011-09, Vol.83 (17), p.6586-6592 |
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| creator | Zheng, Jing Li, Jishan Jiang, Ying Jin, Jianyu Wang, Kemin Yang, Ronghua Tan, Weihong |
| description | For successful assay development of an aptamer-based biosensor, various design principles and strategies, including a highly selective molecular recognition element and a novel signal transduction mechanism, have to be engineered together. Herein, we report a new type of aptamer-based sensing platform which is based on a triple-helix molecular switch (THMS). The THMS consists of a central, target specific aptamer sequence flanked by two arm segments and a dual-labeled oligonucleotide serving as a signal transduction probe (STP). The STP is doubly labeled with pyrene at the 5′- and 3′-end, respectively, and initially designed as a hairpin-shaped structure, thus, bringing the two pyrenes into spacer proximity. Bindings of two arm segments of the aptamer with the loop sequence of STP enforce the STP to form an “open” configuration. Formation of aptamer/target complex releases the STP, leading to new signal readout. To demonstrate the feasibility and universality of our design, three aptamers which bind to human α-thrombin (Tmb), adenosine triphosphate (ATP), and l-argininamide (L-Arm), respectively, were selected as models. The universality of the approach is achieved by virtue of altering the aptamer sequence without change of the triple-helix structure. |
| doi_str_mv | 10.1021/ac201314y |
| format | Article |
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Herein, we report a new type of aptamer-based sensing platform which is based on a triple-helix molecular switch (THMS). The THMS consists of a central, target specific aptamer sequence flanked by two arm segments and a dual-labeled oligonucleotide serving as a signal transduction probe (STP). The STP is doubly labeled with pyrene at the 5′- and 3′-end, respectively, and initially designed as a hairpin-shaped structure, thus, bringing the two pyrenes into spacer proximity. Bindings of two arm segments of the aptamer with the loop sequence of STP enforce the STP to form an “open” configuration. Formation of aptamer/target complex releases the STP, leading to new signal readout. To demonstrate the feasibility and universality of our design, three aptamers which bind to human α-thrombin (Tmb), adenosine triphosphate (ATP), and l-argininamide (L-Arm), respectively, were selected as models. The universality of the approach is achieved by virtue of altering the aptamer sequence without change of the triple-helix structure.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac201314y</identifier><identifier>PMID: 21793587</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Adenosine Triphosphate - chemistry ; Analytical chemistry ; Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - metabolism ; Arginine - analogs & derivatives ; Arginine - chemistry ; Binding sites ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Cell physiology ; Chemical compounds ; Chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Humans ; Kinetics ; Methods. Procedures. Technologies ; Molecular and cellular biology ; Molecular structure ; Oligonucleotides - chemistry ; Protein Binding ; Pyrenes - chemistry ; Signal transduction ; Spectrometry, Fluorescence ; Thermodynamics ; Thrombin - chemistry ; Thrombin - metabolism ; Various methods and equipments</subject><ispartof>Analytical chemistry (Washington), 2011-09, Vol.83 (17), p.6586-6592</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Sep 1, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a371t-c400cb68f02f28bf66132f066f920aef84d967d707e7fc5699b000450e8f258c3</citedby><cites>FETCH-LOGICAL-a371t-c400cb68f02f28bf66132f066f920aef84d967d707e7fc5699b000450e8f258c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac201314y$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac201314y$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24483709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21793587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Jing</creatorcontrib><creatorcontrib>Li, Jishan</creatorcontrib><creatorcontrib>Jiang, Ying</creatorcontrib><creatorcontrib>Jin, Jianyu</creatorcontrib><creatorcontrib>Wang, Kemin</creatorcontrib><creatorcontrib>Yang, Ronghua</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><title>Design of Aptamer-Based Sensing Platform Using Triple-Helix Molecular Switch</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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To demonstrate the feasibility and universality of our design, three aptamers which bind to human α-thrombin (Tmb), adenosine triphosphate (ATP), and l-argininamide (L-Arm), respectively, were selected as models. The universality of the approach is achieved by virtue of altering the aptamer sequence without change of the triple-helix structure.</description><subject>Adenosine Triphosphate - chemistry</subject><subject>Analytical chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Aptamers, Nucleotide - metabolism</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - chemistry</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cell physiology</subject><subject>Chemical compounds</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Molecular structure</subject><subject>Oligonucleotides - chemistry</subject><subject>Protein Binding</subject><subject>Pyrenes - chemistry</subject><subject>Signal transduction</subject><subject>Spectrometry, Fluorescence</subject><subject>Thermodynamics</subject><subject>Thrombin - chemistry</subject><subject>Thrombin - metabolism</subject><subject>Various methods and equipments</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0FtLwzAUB_AgipvTB7-AFEHEh-pJ2ibp45yXCROFbc8lTZPZkV5MWnTf3urmBvp0OPDjXP4InWK4xkDwjZAEcIDD1R7q44iATzkn-6gPAIFPGEAPHTm3BMAYMD1EPYJZHESc9dHkTrl8UXqV9oZ1Iwpl_VvhVOZNVenycuG9GtHoyhbe_Ked2bw2yh8rk396z5VRsjXCetOPvJFvx-hAC-PUyaYO0PzhfjYa-5OXx6fRcOKLgOHGlyGATCnXQDThqaYUB0QDpTomIJTmYRZTljFgimkZ0ThOu0_CCBTXJOIyGKDL9dzaVu-tck1S5E4qY0SpqtYlnLMIgFHo5PkfuaxaW3bHdSiOMQGOO3S1RtJWzlmlk9rmhbCrBEPyHXCyDbizZ5uBbVqobCt_E-3AxQYIJ4XRVpQydzsXhjxgEO-ckG531P-FX6l6jCM</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Zheng, Jing</creator><creator>Li, Jishan</creator><creator>Jiang, Ying</creator><creator>Jin, Jianyu</creator><creator>Wang, Kemin</creator><creator>Yang, Ronghua</creator><creator>Tan, Weihong</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>Design of Aptamer-Based Sensing Platform Using Triple-Helix Molecular Switch</title><author>Zheng, Jing ; Li, Jishan ; Jiang, Ying ; Jin, Jianyu ; Wang, Kemin ; Yang, Ronghua ; Tan, Weihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a371t-c400cb68f02f28bf66132f066f920aef84d967d707e7fc5699b000450e8f258c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenosine Triphosphate - chemistry</topic><topic>Analytical chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Aptamers, Nucleotide - metabolism</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - chemistry</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cell physiology</topic><topic>Chemical compounds</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Molecular structure</topic><topic>Oligonucleotides - chemistry</topic><topic>Protein Binding</topic><topic>Pyrenes - chemistry</topic><topic>Signal transduction</topic><topic>Spectrometry, Fluorescence</topic><topic>Thermodynamics</topic><topic>Thrombin - chemistry</topic><topic>Thrombin - metabolism</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Jing</creatorcontrib><creatorcontrib>Li, Jishan</creatorcontrib><creatorcontrib>Jiang, Ying</creatorcontrib><creatorcontrib>Jin, Jianyu</creatorcontrib><creatorcontrib>Wang, Kemin</creatorcontrib><creatorcontrib>Yang, Ronghua</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Jing</au><au>Li, Jishan</au><au>Jiang, Ying</au><au>Jin, Jianyu</au><au>Wang, Kemin</au><au>Yang, Ronghua</au><au>Tan, Weihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Design of Aptamer-Based Sensing Platform Using Triple-Helix Molecular Switch</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>83</volume><issue>17</issue><spage>6586</spage><epage>6592</epage><pages>6586-6592</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>For successful assay development of an aptamer-based biosensor, various design principles and strategies, including a highly selective molecular recognition element and a novel signal transduction mechanism, have to be engineered together. Herein, we report a new type of aptamer-based sensing platform which is based on a triple-helix molecular switch (THMS). The THMS consists of a central, target specific aptamer sequence flanked by two arm segments and a dual-labeled oligonucleotide serving as a signal transduction probe (STP). The STP is doubly labeled with pyrene at the 5′- and 3′-end, respectively, and initially designed as a hairpin-shaped structure, thus, bringing the two pyrenes into spacer proximity. Bindings of two arm segments of the aptamer with the loop sequence of STP enforce the STP to form an “open” configuration. Formation of aptamer/target complex releases the STP, leading to new signal readout. To demonstrate the feasibility and universality of our design, three aptamers which bind to human α-thrombin (Tmb), adenosine triphosphate (ATP), and l-argininamide (L-Arm), respectively, were selected as models. The universality of the approach is achieved by virtue of altering the aptamer sequence without change of the triple-helix structure.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21793587</pmid><doi>10.1021/ac201314y</doi><tpages>7</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - chemistry Analytical chemistry Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Arginine - analogs & derivatives Arginine - chemistry Binding sites Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Cell physiology Chemical compounds Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Humans Kinetics Methods. Procedures. Technologies Molecular and cellular biology Molecular structure Oligonucleotides - chemistry Protein Binding Pyrenes - chemistry Signal transduction Spectrometry, Fluorescence Thermodynamics Thrombin - chemistry Thrombin - metabolism Various methods and equipments |
| title | Design of Aptamer-Based Sensing Platform Using Triple-Helix Molecular Switch |
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