Regulation of plant cytosolic aldolase functions by redox-modifications

From the five genes which code for cytosolic fructose 1,6-bisphosphate aldolases in Arabidopsis thaliana L., the cDNA clone of cAld2 (At2g36460), was heterologously expressed in E. coli and incubated under various oxidizing and reducing conditions. Covalent binding of a GSH moiety to the enzyme was...

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Veröffentlicht in:	Plant physiology and biochemistry 2011-09, Vol.49 (9), p.946-957
Hauptverfasser:	van der Linde, Karina, Gutsche, Nora, Leffers, Hans-Martin, Lindermayr, Christian, Müller, Bernd, Holtgrefe, Simone, Scheibe, Renate
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins - chemistry Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biological and medical sciences Biotin-switch assay Cell Nucleus - enzymology Cell Nucleus - genetics Cytosol - enzymology Cytosolic aldolase Disulfide bridge Escherichia coli - enzymology Escherichia coli - genetics Fructose-Bisphosphate Aldolase - chemistry Fructose-Bisphosphate Aldolase - genetics Fructose-Bisphosphate Aldolase - metabolism Fructosediphosphates - genetics Fructosediphosphates - metabolism Fundamental and applied biological sciences. Psychology Glutathionylation GSNO GSSG Mutation, Missense Nitrosylation Nuclear localization Oxidation-Reduction Plant physiology and development Protein Processing, Post-Translational - physiology Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism ROS Split-YFP Thioredoxin h
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