Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3
Abstract In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo . For determination of stem cell differentiation, RT-PC...
Gespeichert in:
| Veröffentlicht in: | Biomaterials 2011-11, Vol.32 (32), p.8139-8149 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8149 |
|---|---|
| container_issue | 32 |
| container_start_page | 8139 |
| container_title | Biomaterials |
| container_volume | 32 |
| creator | Park, Ji Sun Shim, Myung-Sun Shim, Sung Han Yang, Han Na Jeon, Su Yeon Woo, Dae Gyun Lee, Dong Ryul Yoon, Tae Ki Park, Keun-Hong |
| description | Abstract In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo . For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chondrogenesis. Using these analysis methods, several of the cultured hMSCS were found to highly express genes and proteins specific to cartilage forming tissues. Additionally, similar trends in expression were found in tissue recovered from nude mice transplanted with several types of hMSCs encapsulated in a fibrin hydrogel containing TGF-β3. The results of both in vitro and in vivo analyses showed that cultured or transplanted hMSCs mixed with TGF-β3 in a fibrin hydrogel differentiated into chondrocytes, suggesting that these cells would be suitable for reconstruction of hyaline articular cartilage. |
| doi_str_mv | 10.1016/j.biomaterials.2011.07.043 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_885903345</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S014296121100826X</els_id><sourcerecordid>885903345</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-d4819aedd3420962df580e0a8a2fafd5ca61f5a6cc4ad50c9c7b8e0d875d4ce53</originalsourceid><addsrcrecordid>eNqNUsFu1DAUtBCILoVfQBYXLk2wHTtxOCBVCy1IlThQJG6WY78sXhJ7sZ2ifgX_wofwTTjaghAnTk-WZuZ5Zh5CzyipKaHti309uDDrDNHpKdWMUFqTria8uYc2VHayEj0R99GGUM6qvqXsBD1KaU_Km3D2EJ0wKjkRst-g79vPwdsYduCdwYeQweeiisOIU4YZG5imhG1ZdQMWjzHMWM_ehVzQ47Q4e4a1dYeQAGeX0gJnOEQ8BA941jGGbxi80Ye0TOW_FjuPRzfEMnZQdE3wWTvv_A5fX15UP380j9GDsZiCJ3fzFH28eHO9fVtdvb98tz2_qgynIleWS9prsLbhjPQts6OQBIiWmo16tMLolo5Ct8ZwbQUxvekGCcTKTlhuQDSn6PlR9xDD1wVSVrNLq1ntISxJSVkybBq-Il8ekSaGlCKM6hBd8XarKFFrH2qv_u5DrX0o0qnSRyE_vVuzDDPYP9TfBRTA6yOgxAE3DqJKxpXIwLoIJisb3P_tefWPjJlKrkZPX-AW0j4s0a8cqhJTRH1YL2M9DEoJkaz91PwCTUi8Lg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>885903345</pqid></control><display><type>article</type><title>Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Park, Ji Sun ; Shim, Myung-Sun ; Shim, Sung Han ; Yang, Han Na ; Jeon, Su Yeon ; Woo, Dae Gyun ; Lee, Dong Ryul ; Yoon, Tae Ki ; Park, Keun-Hong</creator><creatorcontrib>Park, Ji Sun ; Shim, Myung-Sun ; Shim, Sung Han ; Yang, Han Na ; Jeon, Su Yeon ; Woo, Dae Gyun ; Lee, Dong Ryul ; Yoon, Tae Ki ; Park, Keun-Hong</creatorcontrib><description>Abstract In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo . For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chondrogenesis. Using these analysis methods, several of the cultured hMSCS were found to highly express genes and proteins specific to cartilage forming tissues. Additionally, similar trends in expression were found in tissue recovered from nude mice transplanted with several types of hMSCs encapsulated in a fibrin hydrogel containing TGF-β3. The results of both in vitro and in vivo analyses showed that cultured or transplanted hMSCs mixed with TGF-β3 in a fibrin hydrogel differentiated into chondrocytes, suggesting that these cells would be suitable for reconstruction of hyaline articular cartilage.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2011.07.043</identifier><identifier>PMID: 21840589</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adipose Tissue - cytology ; Advanced Basic Science ; Amniotic Fluid - cytology ; Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Cartilage ; Cartilage - drug effects ; Cartilage - metabolism ; Cells, Cultured ; Cells, Immobilized - cytology ; Chondrogenesis ; Chondrogenesis - drug effects ; Collagen Type I - metabolism ; Collagen Type II - metabolism ; Dentistry ; Fibrin ; Fibrin - pharmacology ; Gels ; Glycosaminoglycans - metabolism ; hMSC ; Humans ; Immunohistochemistry ; Immunophenotyping ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - cytology ; Mice ; Mice, Nude ; Organ Specificity - drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; TGF-β3 ; Transforming Growth Factor beta3 - pharmacology</subject><ispartof>Biomaterials, 2011-11, Vol.32 (32), p.8139-8149</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-d4819aedd3420962df580e0a8a2fafd5ca61f5a6cc4ad50c9c7b8e0d875d4ce53</citedby><cites>FETCH-LOGICAL-c415t-d4819aedd3420962df580e0a8a2fafd5ca61f5a6cc4ad50c9c7b8e0d875d4ce53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014296121100826X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21840589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Ji Sun</creatorcontrib><creatorcontrib>Shim, Myung-Sun</creatorcontrib><creatorcontrib>Shim, Sung Han</creatorcontrib><creatorcontrib>Yang, Han Na</creatorcontrib><creatorcontrib>Jeon, Su Yeon</creatorcontrib><creatorcontrib>Woo, Dae Gyun</creatorcontrib><creatorcontrib>Lee, Dong Ryul</creatorcontrib><creatorcontrib>Yoon, Tae Ki</creatorcontrib><creatorcontrib>Park, Keun-Hong</creatorcontrib><title>Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo . For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chondrogenesis. Using these analysis methods, several of the cultured hMSCS were found to highly express genes and proteins specific to cartilage forming tissues. Additionally, similar trends in expression were found in tissue recovered from nude mice transplanted with several types of hMSCs encapsulated in a fibrin hydrogel containing TGF-β3. The results of both in vitro and in vivo analyses showed that cultured or transplanted hMSCs mixed with TGF-β3 in a fibrin hydrogel differentiated into chondrocytes, suggesting that these cells would be suitable for reconstruction of hyaline articular cartilage.</description><subject>Adipose Tissue - cytology</subject><subject>Advanced Basic Science</subject><subject>Amniotic Fluid - cytology</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Cartilage</subject><subject>Cartilage - drug effects</subject><subject>Cartilage - metabolism</subject><subject>Cells, Cultured</subject><subject>Cells, Immobilized - cytology</subject><subject>Chondrogenesis</subject><subject>Chondrogenesis - drug effects</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type II - metabolism</subject><subject>Dentistry</subject><subject>Fibrin</subject><subject>Fibrin - pharmacology</subject><subject>Gels</subject><subject>Glycosaminoglycans - metabolism</subject><subject>hMSC</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunophenotyping</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Organ Specificity - drug effects</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>TGF-β3</subject><subject>Transforming Growth Factor beta3 - pharmacology</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUsFu1DAUtBCILoVfQBYXLk2wHTtxOCBVCy1IlThQJG6WY78sXhJ7sZ2ifgX_wofwTTjaghAnTk-WZuZ5Zh5CzyipKaHti309uDDrDNHpKdWMUFqTria8uYc2VHayEj0R99GGUM6qvqXsBD1KaU_Km3D2EJ0wKjkRst-g79vPwdsYduCdwYeQweeiisOIU4YZG5imhG1ZdQMWjzHMWM_ehVzQ47Q4e4a1dYeQAGeX0gJnOEQ8BA941jGGbxi80Ye0TOW_FjuPRzfEMnZQdE3wWTvv_A5fX15UP380j9GDsZiCJ3fzFH28eHO9fVtdvb98tz2_qgynIleWS9prsLbhjPQts6OQBIiWmo16tMLolo5Ct8ZwbQUxvekGCcTKTlhuQDSn6PlR9xDD1wVSVrNLq1ntISxJSVkybBq-Il8ekSaGlCKM6hBd8XarKFFrH2qv_u5DrX0o0qnSRyE_vVuzDDPYP9TfBRTA6yOgxAE3DqJKxpXIwLoIJisb3P_tefWPjJlKrkZPX-AW0j4s0a8cqhJTRH1YL2M9DEoJkaz91PwCTUi8Lg</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Park, Ji Sun</creator><creator>Shim, Myung-Sun</creator><creator>Shim, Sung Han</creator><creator>Yang, Han Na</creator><creator>Jeon, Su Yeon</creator><creator>Woo, Dae Gyun</creator><creator>Lee, Dong Ryul</creator><creator>Yoon, Tae Ki</creator><creator>Park, Keun-Hong</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3</title><author>Park, Ji Sun ; Shim, Myung-Sun ; Shim, Sung Han ; Yang, Han Na ; Jeon, Su Yeon ; Woo, Dae Gyun ; Lee, Dong Ryul ; Yoon, Tae Ki ; Park, Keun-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-d4819aedd3420962df580e0a8a2fafd5ca61f5a6cc4ad50c9c7b8e0d875d4ce53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipose Tissue - cytology</topic><topic>Advanced Basic Science</topic><topic>Amniotic Fluid - cytology</topic><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Cartilage</topic><topic>Cartilage - drug effects</topic><topic>Cartilage - metabolism</topic><topic>Cells, Cultured</topic><topic>Cells, Immobilized - cytology</topic><topic>Chondrogenesis</topic><topic>Chondrogenesis - drug effects</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type II - metabolism</topic><topic>Dentistry</topic><topic>Fibrin</topic><topic>Fibrin - pharmacology</topic><topic>Gels</topic><topic>Glycosaminoglycans - metabolism</topic><topic>hMSC</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunophenotyping</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Organ Specificity - drug effects</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>TGF-β3</topic><topic>Transforming Growth Factor beta3 - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Ji Sun</creatorcontrib><creatorcontrib>Shim, Myung-Sun</creatorcontrib><creatorcontrib>Shim, Sung Han</creatorcontrib><creatorcontrib>Yang, Han Na</creatorcontrib><creatorcontrib>Jeon, Su Yeon</creatorcontrib><creatorcontrib>Woo, Dae Gyun</creatorcontrib><creatorcontrib>Lee, Dong Ryul</creatorcontrib><creatorcontrib>Yoon, Tae Ki</creatorcontrib><creatorcontrib>Park, Keun-Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Ji Sun</au><au>Shim, Myung-Sun</au><au>Shim, Sung Han</au><au>Yang, Han Na</au><au>Jeon, Su Yeon</au><au>Woo, Dae Gyun</au><au>Lee, Dong Ryul</au><au>Yoon, Tae Ki</au><au>Park, Keun-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2011-11</date><risdate>2011</risdate><volume>32</volume><issue>32</issue><spage>8139</spage><epage>8149</epage><pages>8139-8149</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-β3 and then evaluated for their capacity for differentiation in vitro and in vivo . For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chondrogenesis. Using these analysis methods, several of the cultured hMSCS were found to highly express genes and proteins specific to cartilage forming tissues. Additionally, similar trends in expression were found in tissue recovered from nude mice transplanted with several types of hMSCs encapsulated in a fibrin hydrogel containing TGF-β3. The results of both in vitro and in vivo analyses showed that cultured or transplanted hMSCs mixed with TGF-β3 in a fibrin hydrogel differentiated into chondrocytes, suggesting that these cells would be suitable for reconstruction of hyaline articular cartilage.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>21840589</pmid><doi>10.1016/j.biomaterials.2011.07.043</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2011-11, Vol.32 (32), p.8139-8149 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_885903345 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adipose Tissue - cytology Advanced Basic Science Amniotic Fluid - cytology Animals Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Cartilage Cartilage - drug effects Cartilage - metabolism Cells, Cultured Cells, Immobilized - cytology Chondrogenesis Chondrogenesis - drug effects Collagen Type I - metabolism Collagen Type II - metabolism Dentistry Fibrin Fibrin - pharmacology Gels Glycosaminoglycans - metabolism hMSC Humans Immunohistochemistry Immunophenotyping Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - cytology Mice Mice, Nude Organ Specificity - drug effects Reverse Transcriptase Polymerase Chain Reaction TGF-β3 Transforming Growth Factor beta3 - pharmacology |
| title | Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T01%3A29%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chondrogenic%20potential%20of%20stem%20cells%20derived%20from%20amniotic%20fluid,%20adipose%20tissue,%20or%20bone%20marrow%20encapsulated%20in%20fibrin%20gels%20containing%20TGF-%CE%B23&rft.jtitle=Biomaterials&rft.au=Park,%20Ji%20Sun&rft.date=2011-11&rft.volume=32&rft.issue=32&rft.spage=8139&rft.epage=8149&rft.pages=8139-8149&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/j.biomaterials.2011.07.043&rft_dat=%3Cproquest_cross%3E885903345%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=885903345&rft_id=info:pmid/21840589&rft_els_id=S014296121100826X&rfr_iscdi=true |